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EC number: 201-964-7 | CAS number: 90-05-1
Acute Toxicity: inhalation
Administrative data
- Endpoint:
- acute toxicity: inhalation
- Type of information:
- experimental study
- Adequacy of study:
- other information
- Reliability:
- 4 (not assignable)
- Rationale for reliability incl. deficiencies:
- other: see 'Remark'
- Remarks:
- The paper was published in Russian but an English translation was presented by RIFM. The purity of the test substance is unknown, the system of vapour generation was not described in details and control animals were not mentioned. Furthermore, this study is performed on mice and only after 2 hours of exposure. For these reasons, a reliability 4 was assigned. The GLP were not mentioned but the study was performed before the GLP standard was established.
Data source
Referenceopen allclose all
- Reference Type:
- publication
- Title:
- Monographs on fragrance raw materials
- Author:
- Opdyke DL, Letizia C.
- Year:
- 1 982
- Bibliographic source:
- Food Chem Toxicol, 20, 697-701.
- Reference Type:
- publication
- Title:
- Toxicology of guaiacol vapors and of its resin
- Author:
- Ostrovsky M.M.
- Year:
- 1 964
- Bibliographic source:
- Journal Gigiena i Sanitariya, 29, 85-88. (Russian)
- Reference Type:
- review article or handbook
- Title:
- Gaiacol
- Author:
- RIFM ([url]: http://rifm.org/nd/mono.cfm?CASNumber=90-05-1)
- Year:
- 1 982
- Bibliographic source:
- Monograph: 9 (Published 1982: FCT,v20,p697, Special Issue VI)
Materials and methods
Test guideline
- Qualifier:
- no guideline followed
- Principles of method if other than guideline:
- Acute toxicity inhalation
- GLP compliance:
- not specified
- Test type:
- other: acute toxicity inhalation
- Limit test:
- no
Test material
- Reference substance name:
- Guaiacol
- EC Number:
- 201-964-7
- EC Name:
- Guaiacol
- Cas Number:
- 90-05-1
- Molecular formula:
- C7H8O2
- IUPAC Name:
- Phenol
- Details on test material:
- The origin of guaiacol was not reported and its purity was unknown.
Constituent 1
Test animals
- Species:
- mouse
- Strain:
- not specified
- Sex:
- not specified
Administration / exposure
- Route of administration:
- inhalation: vapour
- Type of inhalation exposure:
- not specified
- Vehicle:
- other:
- Analytical verification of test atmosphere concentrations:
- not specified
- Duration of exposure:
- 2 h
- Concentrations:
- 2.03, 3.6, 4, 6.13, 6.42, 7.57, 7.74, 7.76, 9.53, 12.64, 17.19 mg/L
- No. of animals per sex per dose:
- 70 mice in the experiment
- Control animals:
- not specified
Results and discussion
Effect levelsopen allclose all
- Sex:
- not specified
- Dose descriptor:
- LC50
- Effect level:
- 7.57 mg/L air
- Exp. duration:
- 2 h
- Remarks on result:
- other: The survival rate of the animals was the criterion for evaluating toxicity.
- Sex:
- not specified
- Dose descriptor:
- LC50
- Effect level:
- 3.78 mg/L air
- Exp. duration:
- 4 h
- Remarks on result:
- other: Calculated
- Mortality:
- From 7.76 mg/L guaiacol vapours to 12.64 mg/L, the majority of animals died. At 17.19 mg/L, all the animals died and it was considered as LC100.
- Clinical signs:
- other: In the initial period of the substance effects, all the mice showed slight inflammation of respiratory tract and eye mucous membranes. These symptoms were accompanied by short-term nervousness, followed by little activity. These clinical signs were rever
- Gross pathology:
- Guaiacol vapors showed also damage to the vascularization of the internal organs and bleeding into the membrane and brain matter. Dystrophic
changes were rarer: protein dystrophy of the heart muscle and focal fat of the parenchyma of the liver.
Applicant's summary and conclusion
- Interpretation of results:
- study cannot be used for classification
- Executive summary:
In an acute inhalation toxicity study (Ostrovsky, 1964), groups of mice were exposed by inhalation route to guaiacol for 2 hours at concentrations of 2.03, 3.6, 4, 6.13, 6.42, 7.57, 7.74, 7.76, 9.53, 12.64, 17.19 mg/L.
The LC50 = 7.57 mg/L for 2 hours (calculated as 3.78 mg/L for 4 hours) .
Based on this data, no conclusion on guaiacol classification can be drawn for this endpoint, since this study was performed in mice instead of rats and during only 2 hours instead of 4 as required in the OECD guideline.
Guaiacol was officially classified in the Regulation (EC) n°1272/2008 Annex VI table 3 and was not classified for acute toxicity by inhalation.
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