N,N'-ethylenebis[N-acetylacetamide]

Toxicity to microorganisms

Administrative data

Endpoint:

activated sludge respiration inhibition testing

Type of information:

experimental study

Adequacy of study:

key study

Study period:

1992-11-06

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

other: The study was performed according to OECD guideline 209. It followed the principles of GLP, but some parts of the study were not fully conducted under GLP.

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1992

Report date:

1992

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

OECD Guideline 209 (Activated Sludge, Respiration Inhibition Test

Deviations:

not specified

GLP compliance:

no

Remarks:

but GLP conform

Test material

Test material information

Constituent 1

Reference substance name:

N,N'-ethylenebis[N-acetylacetamide]

EC Number:

234-123-8

EC Name:

N,N'-ethylenebis[N-acetylacetamide]

Cas Number:

10543-57-4

Molecular formula:

C10H16N2O4

IUPAC Name:

N,N'-ethylenebis[N-acetylacetamide]

Details on test material:

- Name of test material (as cited in study report): T A E D
- Analytical purity: 99 +/- 0.5 %
- Purity test date: 24.08.1992
- Lot/batch No.: 24081

Sampling and analysis

Analytical monitoring:

no

Test solutions

Vehicle:

no

Details on test solutions:

No test solution was prepared. The test material was given directly to the test vessels.

Test organisms

Test organisms (species):

activated sludge of a predominantly domestic sewage

Details on inoculum:

Origin: obtained from a local sewage processing plant (handles with predominantly domestic sewage) of Frankfurt-Sprendlingen, Germany
Maintenance before test start: according to corresponding Guideline
Acclimatization: not acclimatized to test item or reference substance

Study design

Test type:

static

Water media type:

not specified

Limit test:

no

Total exposure duration:

3 h

Test conditions

Hardness:

no data

Test temperature:

20 +- 2°C

pH:

7.2 +- 0.2

Dissolved oxygen:

no data

Salinity:

no data

Nominal and measured concentrations:

500 mg TAED/L to 1000 mg TAED/L (nominal)

Details on test conditions:

TEST SYSTEM
- Test vessel:
- normal laboratory equipment,
- Aeration: yes, 0.5 to 1 litre per minute and test vessel.
- No. of vessels per concentration (replicates): 1
- No. of vessels per control (replicates): 2
- Biomass loading rate: 1600 mg/L dry weight of microbial innoculum


EFFECT PARAMETERS MEASURED: respiration rate after 3 hours


TEST CONCENTRATIONS
- Spacing factor for test concentrations: < 3.2
- test item concentrations were between 500 and 100 mg/L

Reference substance (positive control):

yes

Remarks:

3,5-Dichlorophenol

Results and discussion

Effect concentrations

Effect concentrations 1

Duration:

3 h

Dose descriptor:

EC50

Effect conc.:

> 1 000 mg/L

Effect concentrations 2

Duration:

3 h

Dose descriptor:

other: EC20

Effect conc.:

> 1 000 mg/L

Effect concentrations 3

Duration:

3 h

Dose descriptor:

other: EC80

Effect conc.:

> 1 000 mg/L

Results with reference substance (positive control):

The respiration rates of the controls were within the accepted range.

Reported statistics and error estimates:

no data

Applicant's summary and conclusion

Validity criteria fulfilled:

yes

Conclusions:

TAED, at concentrations up to 1000 mg/L, exhibited no acute toxic effects towards the microbiological inoculum.

Executive summary:

This study was designed to determine the effects o f TAED on sewage sludge micro-organisms by measuring the respiration rate after exposure to the test substance at concentrations of 500 to 1000 mg/L after a 3 h contact time.

TAED, at concentrations up to 1000 mg/L, exhibited no acute toxic effects towards the microbiological inoculum.