N,N'-ethylenebis[N-acetylacetamide]

Short-term toxicity to aquatic invertebrates

Administrative data

Endpoint:

short-term toxicity to aquatic invertebrates

Type of information:

experimental study

Adequacy of study:

key study

Study period:

May 14-16, 2002

Reliability:

1 (reliable without restriction)

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

2002

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

EPA OPPTS 850.1010 (Aquatic Invertebrate Acute Toxicity Test, Freshwater Daphnids)

Deviations:

no

GLP compliance:

yes

Test material

Test material information

Constituent 1

Reference substance name:

N,N'-ethylenebis[N-acetylacetamide]

EC Number:

234-123-8

EC Name:

N,N'-ethylenebis[N-acetylacetamide]

Cas Number:

10543-57-4

Molecular formula:

C10H16N2O4

IUPAC Name:

N,N'-ethylenebis[N-acetylacetamide]

Details on test material:

- Name of test material (as cited in study report): tetraacetyl ethylene diamine
- Physical state: white to pale cream powder
- Analytical purity: 99 %
- Lot/batch No.: 13405002
- Expiration date of the lot/batch: not specified at time of receipt (18 April 2002)

Sampling and analysis

Analytical monitoring:

yes

Details on sampling:

- Concentrations: samples were taken from each test medium (start of exposure) and each replicate test vessel (end of exposure)
- Sampling method: samples were taken at the start of exposure period and at the end of exposure
- Sample storage conditions before analysis: not applicable

Test solutions

Vehicle:

no

Details on test solutions:

A 1000 mg/L aqueous test medium was prepared by the direct addition of tetraacetyl ethylene diamine to ASTM medium. The test medium was sonicated to aid dissolution. The remaining test media were produced by serial dilution of the 1000 mg/L test medium with ASTM medium.
The control treatment was prepared by the addition ofASTM medium only to the test vessels .

Test organisms

Test organisms (species):

Daphnia magna

Details on test organisms:

TEST ORGANISM
- Common name: Daphnia magna
- Source: laboratory culture derived from a clone held at Sheffield University, Sheffield, UK
- Age at study initiation: less than 24 h old


ACCLIMATION
- Acclimation period: less than 24 hours
- Acclimation conditions: same as test

Study design

Test type:

static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

48 h

Test conditions

Hardness:

Total hardness as CaCO3: 171 mg/L

Test temperature:

19.7 - 20.8 °C

pH:

7.0 - 7.6

Dissolved oxygen:

70 - 99 % (air saturated value)

Salinity:

no data

Nominal and measured concentrations:

nominal: 63, 125, 250, 500 and 1000 mg/L
measured: 63.1, 126, 259, 530 and 1046 mg/L, equivalent to 100, 101, 104, 106 and 105% respectively of the nominal exposure concentrations.

Details on test conditions:

TEST SYSTEM
- Test vessel:
- Type: 250 mL glass beakers
- Aeration: none
- No. of organisms per vessel: 10
- No. of vessels per concentration (replicates): 2
- No. of vessels per control (replicates): 2


TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: standard hard water (ASTM-medium)
- Total organic carbon: Sodium hydrogen carbonate, 192 mg/L
- Metals: Magnesium sulphate heptahydrate, 256 mg/L; Calcium sulphate dihydrate, 120 mg/L
- Pesticides: not applicable
- Chlorine: Potassium Cloride, 8 mg/L
- Alkalinity: 86 - 116 mg/L as CaCO3
- Culture medium different from test medium: no
- Intervals of water quality measurement: not applicable


OTHER TEST CONDITIONS
- Adjustment of pH: no
- Photoperiod: 16 h light : 8 h dark
- Light intensity: not applicable


EFFECT PARAMETERS MEASURED (with observation intervals if applicable) : numbers of immobilisied


TEST CONCENTRATIONS
- Spacing factor for test concentrations: 2

Reference substance (positive control):

no

Results and discussion

Effect concentrations

Effect concentrations 1

Duration:

48 h

Dose descriptor:

EC50

Effect conc.:

> 1 000 mg/L

Nominal / measured:

meas. (arithm. mean)

Conc. based on:

test mat.

Basis for effect:

mobility

Effect concentrations 2

Duration:

48 h

Dose descriptor:

LOEC

Effect conc.:

1 000 mg/L

Nominal / measured:

meas. (arithm. mean)

Conc. based on:

test mat.

Basis for effect:

mobility

Results with reference substance (positive control):

not applicable

Reported statistics and error estimates:

no statistics performed, due to results

Applicant's summary and conclusion

Validity criteria fulfilled:

yes

Conclusions:

No immobility of Daphnia magna was observed in any of the test media containing tetraacetyl ethylene diamine. Therefore, the 48 hour EC50 value of tetraacetyl ethylene diamine was observed to be >1000 mg/L (the highest nominal concentration tested) .
The lowest concentration causing 100% immobilisation could not be determined and the highest concentration causing no immobilisation was 1000 mg/L (the highest nominal concentration tested).

Executive summary:

The acute toxicity of tetraacetyl ethylene diamine to Daphnia magna was determined at approximately 20°C in a 48 hour static test without renewal of the test media. The study was conducted as a definitive test, with nominal concentrations of 63, 125, 250, 500 and 1000 mg/L.

No immobility of Daphnia magna was observed in any of the test media containing tetraacetyl ethylene diamine. Therefore, the 48 hour EC50 value of tetraacetyl ethylene diamine was observed to be >1000 mg/L (the highest nominal concentration tested). The lowest concentration causing 100% immobilisation could not be determined and the highest concentration causing no immobilisation was 1000 mg/L (the highest nominal concentration tested) .