N,N'-ethylenebis[N-acetylacetamide]

Endpoint summary

Additional information

Toxicokinetics after oral intubation of 14C-labelled TAED were studied in male and female rats. At one hour after administration more than 50 % of the administered radioactivity had been absorbed form the intestine. Tissue levels were at their highest at 2 hours after dosing. Subsequently levels fell rapidly except in the adrenal gland where 14C levels rose during the 7 hours after dosing and declined thereafter. When the radiolabel was in the acetyl moiety, two days after administration 67 % of the radioactivity were eliminated via urine, 3 % via feces and 18 % were expired. In the carcass remained 3 %, which can be attributed to the integration of the acetyl moiety in the C2 pool. Total recovery was 91 %. When the radiolabel was in the ethylene diamine moiety, four days after administration 98 % of the radioactivity were eliminated via urine, 3 % via feces and 0.4 % were expired. 1 % remained in the carcass. Total recovery was 102.5 %.

More than 90 % of the urinary 14C was excreted within 24 hours after dosing, mostly as triacetyl ethylene diamine and diacetyl ethylene diamine. TAED was only present in traces, monoacetyl ethylene diamine and ethylene diamine were not found in the urine.

There was no sex difference in absorption, metabolism and excretion.

Skin penetration studies with TA[14C]ED in detergent solutions and in chloroform were conducted in rats. Clipped dorsal skin was exposed to the detergent formulations for 1, 5, 10 and 20 min and then rinsed. The formulation in chloroform was left on the skin without rinsing. Skin penetration two days after exposure start depended on exposure time and ranged from 0.13 % for the 1 min exposure up to 4.5 % for the continuous exposure. Of the TA[14C]ED which penetrated skin, most was recovered in the urine while less than 3 % was recovered in feces.

Repeated dose toxicity studies in the rat indicate that TAED is partially systemically available following inhalation of TAED dust. Conservatively, it can be assumed that inhalation absorption is 100 %.

The results show that TAED is rapidly and quantitatively absorbed from the intestine, largely metabolized via deacetylation to triacetyl and diacetyl ethylene diamine and rapidly excreted predominately via urine. Thus, it can be concluded that TAED does not have a bioaccumulation potential. Rat skin penetration was between 0.13 % for a 1 min exposure and 4.3 % for a continuous two day exposure. Inspirable TAED dust is partially bioavailabe. Conservatively, inhalation absorption can be assumed to be 100 %.