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Ecotoxicological information

Short-term toxicity to fish

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Administrative data

Endpoint:
short-term toxicity to fish
Type of information:
migrated information: read-across from supporting substance (structural analogue or surrogate)
Adequacy of study:
supporting study
Study period:
1986
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
other: 2e. Study well documented, meets generally accepted scientific principles

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
1986
Report date:
1986

Materials and methods

Test guideline
Qualifier:
no guideline followed
Principles of method if other than guideline:
Test method according to Könemann H., 1981. Fish toxicity tests with mixtures of more than two chemicals: A proposal for a quantitative approach and experimental results. Toxicology Volume 19, Issue 3, 1981, Pages 229-238.
GLP compliance:
no

Test material

Constituent 1
Chemical structure
Reference substance name:
Aniline
EC Number:
200-539-3
EC Name:
Aniline
Cas Number:
62-53-3
Molecular formula:
C6H7N
IUPAC Name:
aniline
Constituent 2
Reference substance name:
2-methylaniline
IUPAC Name:
2-methylaniline
Constituent 3
Reference substance name:
3-methylaniline
IUPAC Name:
3-methylaniline
Constituent 4
Reference substance name:
4-methylaniline
IUPAC Name:
4-methylaniline
Details on test material:
Aniline: purity = pro analysis; LogPoct=1.03 (0.90); Mol weight= 93.13;Chs= 0.00
2-methylaniline: purity >= 99.5%; LogPoct=1.54; Mol weight= 107.16;Chs= 0.10
3-methylaniline: purity >= 99%; LogPoct=1.54; Mol weight= 107.16;Chs= - 0.07
4-methylaniline: purity > 97%; LogPoct=1.54; Mol weight= 107.16;Chs= -0.17
Chs: Hammett sigma constant, which is dependent on the position of the substituent.

Sampling and analysis

Analytical monitoring:
no

Test solutions

Vehicle:
yes

Test organisms

Test organisms (species):
Poecilia reticulata

Study design

Test type:
semi-static
Water media type:
freshwater
Limit test:
no
Total exposure duration:
14 d

Results and discussion

Effect concentrationsopen allclose all
Duration:
14 d
Dose descriptor:
LC50
Effect conc.:
125.6 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mortality (fish)
Remarks on result:
other: Aniline
Duration:
14 d
Dose descriptor:
LC50
Effect conc.:
81.3 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mortality (fish)
Remarks on result:
other: 2-Methylaniline
Duration:
14 d
Dose descriptor:
LC50
Effect conc.:
36.3 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mortality (fish)
Remarks on result:
other: 3-Methylaniline
Duration:
14 d
Dose descriptor:
LC50
Effect conc.:
10.7 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mortality (fish)
Remarks on result:
other: 4-Methylaniline

Applicant's summary and conclusion

Validity criteria fulfilled:
yes
Remarks:
No mortality in the controls.
Conclusions:
Aniline appeared to be slightly toxic to fish (LC50> 100 mg/L), while the methylated anilines were more toxic with LC50 values between 10 and 100 mg/L.