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EC number: 202-870-9 | CAS number: 100-61-8
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data

Long-term toxicity to fish
Administrative data
- Endpoint:
- fish short-term toxicity test on embryo and sac-fry stages
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- other: Comparable to guideline study without detailed documentations.
Data source
Reference
- Reference Type:
- publication
- Title:
- Unnamed
- Year:
- 1 993
Materials and methods
Test guideline
- Qualifier:
- equivalent or similar to guideline
- Guideline:
- OECD Guideline 212 (Fish, Short-term Toxicity Test on Embryo and Sac-Fry Stages)
- GLP compliance:
- not specified
- Remarks:
- .
Test material
- Reference substance name:
- N-methylaniline
- EC Number:
- 202-870-9
- EC Name:
- N-methylaniline
- Cas Number:
- 100-61-8
- Molecular formula:
- C7H9N
- IUPAC Name:
- N-methylaniline
- Details on test material:
- - Name of test material (as cited in study report): N-methylaniline (100-61-8)
- Physical state: solved in reconstituted water
- Analytical purity: reagent grade
- Supplier: Merck, 6100 DarmstacE/FR Germany
Constituent 1
Sampling and analysis
- Analytical monitoring:
- no
Test solutions
- Vehicle:
- no
- Details on test solutions:
- No details specified.
Test organisms
- Test organisms (species):
- Danio rerio (previous name: Brachydanio rerio)
- Details on test organisms:
- TEST ORGANISM
- Common name: Zebrafish
- Strain: Brachydanio rerio
- Source: West Aquarium, 3/422 Bad Lauterberg/FR Germany
METHOD FOR PREPARATION AND COLLECTION OF FERTILIZED EGGS
- Numbers of parental fish to induce spawning: 4 females, 8 males
- Method of collection of fertilised eggs: the spawn was collected on the bottom of a glass box covered by a steel wire-net containing some small green glass trees
- Subsequent handling of eggs: the eggs were separated and cultured at 25°C individually using micro plates (Nunc, Roshilde/Denmark) containing 100 µL reconstituted water.
Study design
- Test type:
- static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 96 h
- Remarks on exposure duration:
- Until hatching.
- Post exposure observation period:
- Not applicable.
Test conditions
- Hardness:
- 200 mg/L
- Test temperature:
- 25°C
- pH:
- 8 ± 0.2
- Dissolved oxygen:
- No data.
- Salinity:
- 2 mM CaCI₂.2 H.O, 0.078 mM KCI, 0.5 mM MgSO₄.7 H₂O, 0.77 mM NHCO₃, in demineralized water
- Nominal and measured concentrations:
- 7 concentrations between 0.1-5 µmol/L
- Details on test conditions:
- TEST SYSTEM
- No. of fertilized eggs/embryos per vessel: 12
- No. of vessels per concentration (replicates): 1
- No. of vessels per control (replicates): 1
EFFECT PARAMETERS MEASURED: marked signs of development, e.g. abnormalities, and deaths during the 96 h test period. - Reference substance (positive control):
- not required
Results and discussion
Effect concentrationsopen allclose all
- Duration:
- 96 h
- Dose descriptor:
- NOEC
- Effect conc.:
- 0.2 µmol/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- other: Oedematous enlargement of the pericardial space with retardation of heart development and reduced blood flow , deformation of the skeleton and the muscle apparatus and general retardation of the embryo development.
- Duration:
- 96 h
- Dose descriptor:
- LC50
- Effect conc.:
- 0.71 µmol/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mortality
- Remarks on result:
- other: 95% CL: 0.69-0.72
- Duration:
- 96 h
- Dose descriptor:
- other: LC100
- Effect conc.:
- 1 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mortality
- Details on results:
- Mortality/survival at embryo and larval stages:
- no deaths or morphological changes were observed in the controls
- an obvious association excisted between malformations and death
Type of morphological abnormalities:
- oedematous enlargement of the pericardial space with retardation of heart development and reduced blood flow
- deformation of the skeleton and the muscle apparatus
- general retardation of the embryo development - Reported statistics and error estimates:
- - oedematous enlargement of the pericardial space in about 20 % of all treated embryos
- deformation of the skeleton and muscle apparatus in approximately one third of all treated embryos
- retardation of body development in about 20 % of all treated embryos
Any other information on results incl. tables
Table 1: Comparison of the toxicity of N-methylaniline to embryonal development of Danio rerio with the results for aniline and N,N-dimethylaniline:
Dimension | Concentration range | NOEL | LC50 | 95%-C.I. | LC100 | Ratio-NOEL | |
Aniline | mmol | 0.2 - 20 | 1 | 9.3 | 9.2-9.5 | 17 | 2.E-04 |
N-Methylaniline | umol | 0.1 - 5 | 0.2 | 0.71 | 0.69-0.72 | 1 | 1 |
N,N-Dimethylaniline | umol | 0.08 - 4 | 0.33 | 1.51 | 1.49-1.54 | 2 | 0.6 |
Hence, the fish-embryotoxicity of N-methylaniline is four orders of magnitude higher compared to aniline, whereas it is in the same range as N,N-dimethylaniline.
Applicant's summary and conclusion
- Validity criteria fulfilled:
- not applicable
- Remarks:
- only similar to guideline
- Conclusions:
- In this study N-methylaniline was found highly toxic to Zebrafish embryo, with a NOEC of 0.2 µmol/L or 21 ug/L and a LC50 of 0.71 µmol/L or 76 ug/L.
- Executive summary:
Zebra-fish embryos exposed for 96 h were more sensitive to methylanilines than to Nmethylamine, N,N-dimethylamine, 2-aminoethanol, and isopropylamine. N-methylanilie, like aniline and N,N-dimethylaniline, produced malformations in the fish embryos with lesions mainly of the heart or skeleton. Besides mortality, effects observed were: a general retardation of the embryo development and oedematous enlargement of the pericardial space with retardation of heart development and reduced blood flow. In addition, exposure to N-methylaniline caused a deformation of the skeleton and the muscle apparatus. The underlying cause of death could not be determined explicitly although an obvious association between malformations and death existed. The NOEL for N-methylaniline was 0.2 µmol/L, while the NOEL for aniline was 1 mmol/L. In conclusion: The embryotoxicity of aniline is enhanced considerably by its alkylation to N-methylaniline or N,N-dimethylaniline. However, NH2-substitution of alkyl compounds leads to agents with less embryotoxicity.
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