Registration Dossier
Registration Dossier
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EC number: 202-870-9 | CAS number: 100-61-8
Toxicity to microorganisms
Administrative data
- Endpoint:
- activated sludge nitrification inhibition testing
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Remarks:
- Data originate from ISO Standard 8192 and are based on an interlaboratory trial performed in 2004.
Data source
Reference
- Reference Type:
- publication
- Title:
- Unnamed
- Year:
- 2 007
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- ISO 8192 (Water quality - Test for inhibition of oxygen consumption by activated sludge for carbonaceous and ammonium oxidation)
- Deviations:
- no
- GLP compliance:
- yes
Test material
- Reference substance name:
- N-methylaniline
- EC Number:
- 202-870-9
- EC Name:
- N-methylaniline
- Cas Number:
- 100-61-8
- Molecular formula:
- C7H9N
- IUPAC Name:
- N-methylaniline
Constituent 1
Sampling and analysis
- Analytical monitoring:
- not specified
Test solutions
- Vehicle:
- not specified
- Details on test solutions:
- Not specified.
Test organisms
- Test organisms (species):
- activated sludge
- Details on inoculum:
- Based on tests performed by various laboratories.
Study design
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 30 min
- Remarks on exposure duration:
- Additional: 3 h
Test conditions
- Reference substance (positive control):
- not required
Results and discussion
Effect concentrationsopen allclose all
- Duration:
- 30 min
- Dose descriptor:
- EC50
- Effect conc.:
- 1.5 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- inhibition of nitrification rate
- Remarks on result:
- other: 95% CL: 1.13 - 1.87 mg/L
- Duration:
- 180 min
- Dose descriptor:
- EC50
- Effect conc.:
- 3.74 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- inhibition of nitrification rate
- Remarks on result:
- other: 95% CL: 1.87 - 5.61 mg/L
Applicant's summary and conclusion
- Conclusions:
- The test substance inhibits nitrification processes in activated sludge at concentrations between 1 and 6 mg/L. According to the ISO Standard 8192, a range of 0.1 to 5 mg/L is considered to be the valid range for the EC50 of the test substance as a reference substance for inhibition of nitrification.
- Executive summary:
In an interlaboratory trial carried out in July 2004 using activated sludge from domestic sewage, data for EC50 values were collected on the inhibition of nitrification during exposure to the test substance for 30 and 180 min. The results were used to determine precision data on the EC50 values and the valid ranges for the use of the test substance as a reference substance. The average EC50 for 30 min. was 1.5 ( ± 0.44) mg/L and the average EC50 for 180 min. (3 h) was 3.74 (± 2.24) mg/L.
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