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Ecotoxicological information

Toxicity to aquatic algae and cyanobacteria

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Description of key information

The 72-h ErC50 is >100 mg/L and the 72-h NOErC = 100 mg/L in freshwater algae.

Key value for chemical safety assessment

EC50 for freshwater algae:
100 mg/L
EC10 or NOEC for freshwater algae:
100 mg/L

Additional information

The toxicity of the test substance towards aquatic algae was investigated in a GLP-compliant test according to OECD 201 (RCC, 1996). In this study, freshwater alga (Scenedesmus subspicatus) were exposed for 72 hours to nominal concentrations of 0 (control), 1.0, 3.2, 10.0, 32.0, and 100 mg test substance/L under static conditions. In addition, as all test media were slightly to strongly coloured depending on the test substance concentration, alga were exposed to medium without test substance under varying reduced light conditions. This was done to differentiate between a direct toxic effect on algal growth, as induced by the test substance, and an indirect effect on growth caused by light absorption of the coloured test substance in solution. The effects on algal growth were similar in both experimental set-ups which demonstrates that the observed effect on algal growth was attributable only to light absorption caused by colouring of the test solutions. Based on these findings, the 72-h EbC50 and ErC50 values are determined at >100 mg/L. The NOEC is 100 mg/L for both endpoints.