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Diss Factsheets

Toxicological information

Genetic toxicity: in vivo

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Administrative data

in vivo mammalian cell study: DNA damage and/or repair
Type of genotoxicity: DNA damage and/or repair
Type of information:
experimental study
Adequacy of study:
disregarded due to major methodological deficiencies
Study period:
Not Reported
3 (not reliable)
Rationale for reliability incl. deficiencies:
other: Documentation insufficient for assessment
Cross-referenceopen allclose all
Reason / purpose for cross-reference:
reference to same study
Reason / purpose for cross-reference:
reference to other study

Data source

Reference Type:
Effects of nickel administration on the synthesis of nucleic acids by nuclei isolated from rat liver
Ono H, Matsui H, Ono T, Wada O
Bibliographic source:
J of Toxicol Sci. 4(3):287

Materials and methods

Test guideline
no guideline followed
Principles of method if other than guideline:
Incorporation of3H-dTTP and 3H-UTP by nuclei isolated from normal and regenerating liver of nickel administered rat was compared with those of corresponding controls. The contents of nickel in the nuclei and nucleioli were also determined in an effort to approach the mechanism of carcinogenic action by metals.
GLP compliance:
not specified
Type of assay:
other: synthesis of nucleic acids

Test material

Constituent 1
Chemical structure
Reference substance name:
Nickel dihydroxide
EC Number:
EC Name:
Nickel dihydroxide
Cas Number:
Molecular formula:
nickel(2+) dihydroxide
Details on test material:
- Name of test material (as cited in study report): Ni(OH)2

Test animals

Details on test animals or test system and environmental conditions:
- Weight at study initiation: 100 g

Administration / exposure

Route of administration:
sesame oil
Details on exposure:
40 mg of Ni(OH)2 suspended in sesame oil was injected subcutaneously
Duration of treatment / exposure:
single administration
Frequency of treatment:
Not Applicable
Post exposure period:
up to 13 weeks
Doses / concentrations
Doses / Concentrations:
40 mg
other: injection
No. of animals per sex per dose:
Not Reported
Control animals:
other: corresponding controls referenced but no information provided
Positive control(s):
Not Applicable


Tissues and cell types examined:
Incorporation of 3H-dTTP and 3H-DUTP by nuclei isolated from normal and regenerating liver of nickel administered rat was compared with those of corresponding controls. The contents of nickel in the nuclei and nucleioli were also determined, including a digestion assay to determine binding properties.
Details of tissue and slide preparation:
Not Reported
Evaluation criteria:
Comparison to concurrent controls
Not Reported

Results and discussion

Test results
other: suggestive
not specified
Vehicle controls validity:
not specified
Negative controls validity:
not specified
Positive controls validity:
not specified
Additional information on results:
The synthesis of nucleic acids of normal and regenerating liver was affected after single administration of nickel and the adverse effects continued for 10-13 weeks, when the contents of nickel in the liver returned to a control level.

The contents of nickel in the nuclei and nucleoli per g of tissue was not increased by the administration of nickel throughout the experiment. However, the content of nickel n the nucleioli expressed as per mg protein, DNA or RNA, respectively, was about two times more than the control for 3 days to 11 weeks after administration.

Digestion of nucleioli by RNase and DNase indicated that almost nickel in the nucleoli bound to sites indigestible to RNase or DNase.

Any other information on results incl. tables

Not Applicable

Applicant's summary and conclusion

Interpretation of results (migrated information): other: suggestive of genotoxicity (as stated by authors)
The authors conclude that results might suggest that nickel affects directly the structure or function of the nucleoli, or indirectly through the change of binding site of nickel in the nucleoli to alter the ability of nucleic acids synthesis in the nuclei. It may not also be ruled out the possibility that nickel in the cytoplasm affects the biological activity of the nuclei.
Executive summary:

Study rated by an independent reviewer.