Dioctyltin oxide

Long-term toxicity to aquatic invertebrates

Link to relevant study record(s)

Reference

Endpoint:

long-term toxicity to aquatic invertebrates

Type of information:

experimental study

Adequacy of study:

key study

Study period:

10 July 2020 - 07 October 2020

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

guideline study

Qualifier:

according to guideline

Guideline:

OECD Guideline 211 (Daphnia magna Reproduction Test)

Version / remarks:

OECD Guideline 211 for Testing of Chemicals (2012)

Deviations:

no

GLP compliance:

yes (incl. QA statement)

Specific details on test material used for the study:

batch number: SCDD161215

Analytical monitoring:

yes

Vehicle:

yes

Remarks:

The test item was administered to the test system via one part of the food (fish food), which was spiked with the test item

Details on test solutions:

Since the test item is insoluble in water, a dietary exposure was carried out. The test item was administered to the test system via one part of the food (fish food), which was spiked with the test item. The loading rate of the test item in the spiked fish food was 200 μg/g, which results in a total loading rate of 4.00 μg/L in the test vessels in aqueous solution. The nominal loading rate of the test item Dioctyltinoxide (DOTO) in the test vessels of 4.00 μg/L was used for the evaluation of the endpoints.

Test organisms (species):

Daphnia magna

Details on test organisms:

Daphnia magna STRAUS

Test type:

semi-static

Water media type:

freshwater

Limit test:

yes

Total exposure duration:

21 d

Hardness:

Total Hardness [mg/L] as CaCO3: between 236 and 281

Test temperature:

Temperature [°C]: between 19.7 and 21.1

pH:

pH between 7.40 and 8.24

Dissolved oxygen:

Dissolved Oxygen Concentration [mg/L]: between 6.74 and 8.92

Reference substance (positive control):

yes

Remarks:

Solvent control: Since octane was used as a solvent to spike the food for the test group, the solvent control group food was treated in the same way but with the absence of test item.

Key result

Duration:

21 d

Dose descriptor:

LOELR

Effect conc.:

> 4 µg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

reproduction

Key result

Duration:

21 d

Dose descriptor:

LOELR

Effect conc.:

>= 4 µg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

reproduction

Key result

Duration:

21 d

Dose descriptor:

other: LOEL Adult mortality

Effect conc.:

> 4 µg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality

Key result

Duration:

21 d

Dose descriptor:

other: NOEL Adult mortality

Effect conc.:

>= 4 µg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality

Details on results:

The overall effect threshold for effects of the test item under the test conditions was ≥ 4.00 μg/L (NOEL) and > 4.00 μg/L (LOEL) based on the nominal loading rate of the test item Dioctyltinoxide (DOTO).

Description of key information

see key study (Daphnia magna Reproduction Test, Semi-static, 21 days, acc. to OECD-Guideline 211 (2012):

The overall effect threshold for effects of the test item under the test conditions was ≥ 4.00 μg/L (NOEL) and > 4.00 μg/L (LOEL) based on the nominal loading rate of the test item Dioctyltinoxide (DOTO)