2-[(2-[2-(dimethylamino)ethoxy]ethyl)methylamino]ethanol

Biodegradation in water: screening tests

Link to relevant study record(s)

Reference

Reference 1

Endpoint:

biodegradation in water: ready biodegradability

Type of information:

experimental study

Adequacy of study:

key study

Study period:

1990-06-15 to 1990-07-13

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

guideline study

Qualifier:

according to guideline

Guideline:

OECD Guideline 301 D (Ready Biodegradability: Closed Bottle Test)

Qualifier:

according to guideline

Guideline:

EU Method C.6 (Degradation: Chemical Oxygen Demand)

GLP compliance:

yes (incl. QA statement)

Specific details on test material used for the study:

- Name of test material (as cited in study report): Texacat ZF-10, 2-[[2-[2-(dimethylamino)ethoxy]ethyl]methylamino]ethanol
- Physical state: Clear colorless liquid
- Analytical purity: 97%
- Lot/batch No.: 90/329
- Expiration date of the lot/batch: approximately two years from receipt (received on 30 March 1990)
- Storage condition of test material: dark at room temperature

Oxygen conditions:

aerobic

Inoculum or test system:

activated sludge, domestic (adaptation not specified)

Details on inoculum:

- Source of inoculum/activated sludge (e.g. location, sampling depth, contamination history, procedure): The aeration stage of the HRC Limited sewage treatment plant treating predominantly domestic sewage collected 15 June 1990.
- Pretreatment: The sample was allowed to settle and the supernatant filtered through Whatman GFA filter paper (first 250 ml discarded)

Duration of test (contact time):

28 d

Initial conc.:

2 mg/L

Based on:

test mat.

Parameter followed for biodegradation estimation:

O2 consumption

Details on study design:

TEST CONDITIONS
- Composition of medium:
(a) Non-inoculated dilution water
(b) Inoculated dilution water
(c) 2 mg/l test substance
(d) 3 mg/l sodium benzoate

Test media b, c & d were inoculated with activated sludge filtrate at the rate of 1 drop of inoculum per litre.


TEST SYSTEM
- Culturing apparatus: 280 ml BOD bottles (darkened glass) with ground glass stoppers.
- Measuring equipment: Yellow Springs BOD Probe

SAMPLING
- Sampling frequency: 0, 5, 15 and 28 days (duplicate bottles at each sampling time).
- Sampling method: Sufficient bottles were prepared to allow a single oxygen determination per bottle

Bottles were incubated in a water bath at 20 ± 1 degree C for 0, 5, 15 or 28 days as appropriate.

Dissolved oxygen concentrations for each test medium were determined in duplicate.

Reference substance:

benzoic acid, sodium salt

Remarks:

Sodium benzoate

Test performance:

Sodium benzoate attained 86% biodegradation within 28 days.
Oxygen depletions in the inoculated and non-inoculated control series were within the prescribed limits.

Key result

Parameter:

% degradation (O2 consumption)

Value:

1

Sampling time:

28 d

Details on results:

1 % degradation after 5 d
1 % degradation after 15 d
1 % degradation after 28 d

Results with reference substance:

76 % degradation after 5 d
83 % degradation after 15 d
86 % degradation after 28 d

Validity criteria fulfilled:

yes

Interpretation of results:

not readily biodegradable

Conclusions:

The test substance attained only 1% biodegradation after 28 days and cannot, therefore, be termed as readily biodegradable.

Reference 2

Endpoint:

biodegradation in water: ready biodegradability

Type of information:

experimental study

Adequacy of study:

supporting study

Study period:

1998-03-23 to 1998-04-20

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

guideline study

Remarks:

Study conducted according to Testing guidelines for Toxicology Studies from Korea and Guidelines of National Institute of Environmental Research and following Korean GLP.

Qualifier:

according to guideline

Guideline:

other: Testing guidelines for Toxicology Studies

Qualifier:

according to guideline

Guideline:

other: Guidelines of National Institute of Environmental Research (Notification No. 1997-9, issued on December 22, 1997)

GLP compliance:

yes (incl. QA statement)

Remarks:

Korean GLP

Specific details on test material used for the study:

- Name of test material (as cited in study report): code No. K-877, 2-((2-(dimethylamino)ethoxyl)methylamino)ethanol, abbreviation JEFFCAT ZF-10
- Appearance: bright liquid
- Purity: 100%
- Lot/batch No.: 186-1-0897

Oxygen conditions:

not specified

Inoculum or test system:

other: collected from 15 sites : river, domestic sewage treatment and industrial wastewater treatment plants, etc

Details on inoculum:

These samples selected from river, domestic sewage treatment and industrial wastewater treatment plants, and etc were poooled then incubated for 1 month to be used in biodegradation test.

Duration of test (contact time):

28 d

Initial conc.:

100 other: ppm

Based on:

not specified

Parameter followed for biodegradation estimation:

O2 consumption

Details on study design:

TEST CONDITIONS
- Concentration of test substance : 100 ppm (w/v)
- Concentration of activated sludge : 30 ppm (w/v)
- Test temperature: 25 +/-2 °C
- Perio : 28 days
- Performed in dark condition

TEST SYSTEM
- bottle 1 and 2 : deionized water 150ml + test substance 100ppm (15mg)
- bottle 3 and 4 : basal culture medium 150 ml + activated sludge 30ppm (dry weight basis) + test substance 100 ppm (15mg)
- bottle 5 : basal culture medium 150 ml + activated sludge 30ppm (dry weight basis) + aniline 100ppm (dry weight basis)
- bottle 6 : basal culture medium 150 ml + activated sludge 30ppm (dry weight basis)

Reference substance:

aniline

Test performance:

The change of BOD concentration of test substance was measured by BOD meter. This result indicated that biodegradation rate of aniline was 67.3% after 7 days, 71.4% after 14 days, which agrees with the acceptable range specified by the OECD Guidelines and the National Institute of Environmental research guidelines.
The test substance was not biodegraded at all after 14 days and 28 days since the BOD concentration was lower than that of control.
The biodegradation rate of aniline by HPLC analysis was 100% after 28 days and the degradation rate of the test substance could not be established.

Results with reference substance:

Biodegradation rate of aniline observed by BOD meter was 67.3% after 7 days and 71.4% after 14 day, which agrees with the acceptable range specified by the OECD guidelines. Biodegradation rate by HPLC analysis was 100% after 28 days.

Validity criteria fulfilled:

not specified

Interpretation of results:

not readily biodegradable

Conclusions:

It is concluded that the test substance may not be readily degraded by microorganism in the aquatic environment judging from the results of the biodegradability test using the BOD meter.

Description of key information

Assessment of Ready Biodegradability of test substance was conducted according to OECD guideline 301D and following GLP requirements.

Under readily biodegradability test, the test substance attained only 1% biodegradation after 28 days and cannot, therefore, be termed as readily biodegradable.

Key value for chemical safety assessment

Biodegradation in water:

under test conditions no biodegradation observed