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Ecotoxicological information

Short-term toxicity to aquatic invertebrates

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Reference
Endpoint:
short-term toxicity to aquatic invertebrates
Type of information:
experimental study
Adequacy of study:
key study
Study period:
1990-07-02 to 1990-07-04
Reliability:
1 (reliable without restriction)
Rationale for reliability incl. deficiencies:
guideline study
Qualifier:
according to guideline
Guideline:
EU Method C.2 (Acute Toxicity for Daphnia)
Version / remarks:
EEC Directive 67/548 Annex V C.2 as published in 84/449/EEC.
Qualifier:
according to guideline
Guideline:
OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)
GLP compliance:
yes (incl. QA statement)
Specific details on test material used for the study:
- Name of test material (as cited in study report): Texacat ZF-10, 2-[[2-[2-(dimethylamino)ethoxy]ethyl]methylamino]ethanol
- Physical state: Clear colorless liquid
- Analytical purity: 97%
- Lot/batch No.: 90/329
- Expiration date of the lot/batch: approximately two years from receipt (received on 30 March 1990)
- Storage condition of test material: dark at room temperature
Analytical monitoring:
yes
Details on sampling:
- Concentrations: Each test concentration verified by chemical analysis (Control, 3.2, 10, 32, 100 and 320 mg/l)
- Sampling method: 100 ml water samples were taken from each test concentration at time 0 and 48 hours.
- Sample storage conditions before analysis: N/A
Vehicle:
no
Details on test solutions:
PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances)
- Method: Direct dispersion in water
Test organisms (species):
Daphnia magna
Details on test organisms:
TEST ORGANISM
- Common name: Water flea
- Strain: Straus
- Source: Laboratory culture originating from astrain supplied by IRCHA, France.
- Age at study initiation (mean and range, SD): Less than 24 hours old
- Method of breeding: Culture conditions ensure reproduction is by parthenogenisis. Gravid adults were isolated 24 hours prior to test initation.
- Feeding during test
- Food type: mixture of fry fish food (Liquify(R)) and a suspension of mixed algae (predominantly Chlorella spp.)
- Amount: N/A
- Frequency: daily
Test type:
static
Water media type:
freshwater
Limit test:
no
Total exposure duration:
48 h
Hardness:
CA 350 mg CaCO3/L
Test temperature:
21 ± 1 °C
pH:
7.8
Nominal and measured concentrations:
3.2, 5.6, 10, 18, 32, 56, 100, 180, and 320 mg/L (nominal)
Details on test conditions:
TEST SYSTEM
- Test vessel: Glass jars
- Material, size, headspace, fill volume: 200 ml test solution
- Aeration: None
- No. of organisms per vessel: 20
- No. of vessels per concentration (replicates): 2
- No. of vessels per control (replicates): 2


TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: Dechlorinated (with sodium thiosulphate) and aged laboratory tap water.


OTHER TEST CONDITIONS
- Photoperiod: 16 hours light : 8 hours dark
Reference substance (positive control):
no
Duration:
24 h
Dose descriptor:
EC50
Effect conc.:
180 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mobility
Remarks on result:
other: 150 - 200 mg/L
Key result
Duration:
48 h
Dose descriptor:
EC50
Effect conc.:
72 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mobility
Remarks on result:
other: 59 - 88 mg/L
Duration:
24 h
Dose descriptor:
NOEC
Effect conc.:
100 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Duration:
48 h
Dose descriptor:
NOEC
Effect conc.:
18 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Validity criteria fulfilled:
not specified
Conclusions:
The 24 and 48 hour EC50 were determined to be 180 mg/L and 72 mg/L respectively while the 24 and 48 hour NOECs were determined to be 100 mg/L and 18 mg/L, respectively.

Description of key information

The Acute Toxicity study of test substance to Daphnia magna was conducted according to OECD guidelines and followed GLP .

The 24 and 48 hour EC50 were determined to be 180 mg/L and 72 mg/L, respectively while the 24 and 48 hour NOECs were determined to be 100 mg/L and 18 mg/L, respectively.

Key value for chemical safety assessment

Fresh water invertebrates

Fresh water invertebrates
Effect concentration:
72 mg/L

Additional information