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EC number: 202-940-9 | CAS number: 101-41-7
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data

Genetic toxicity: in vitro
Administrative data
- Endpoint:
- in vitro gene mutation study in bacteria
- Remarks:
- Type of genotoxicity: gene mutation
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 2000-11-10 to 2001-02-08
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 2 001
- Report date:
- 2001
Materials and methods
Test guidelineopen allclose all
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 471 (Bacterial Reverse Mutation Assay)
- Version / remarks:
- 1997
- Deviations:
- no
- Qualifier:
- according to guideline
- Guideline:
- EU Method B.13/14 (Mutagenicity - Reverse Mutation Test Using Bacteria)
- Deviations:
- no
- GLP compliance:
- yes (incl. QA statement)
- Type of assay:
- bacterial reverse mutation assay
Test material
- Reference substance name:
- Methyl phenylacetate
- EC Number:
- 202-940-9
- EC Name:
- Methyl phenylacetate
- Cas Number:
- 101-41-7
- Molecular formula:
- C9H10O2
- IUPAC Name:
- methyl phenylacetate
Constituent 1
Method
Species / strainopen allclose all
- Species / strain / cell type:
- S. typhimurium TA 1535
- Species / strain / cell type:
- S. typhimurium TA 98
- Species / strain / cell type:
- S. typhimurium, other: TA97a
- Species / strain / cell type:
- S. typhimurium TA 100
- Species / strain / cell type:
- S. typhimurium TA 102
- Metabolic activation:
- with and without
- Metabolic activation system:
- Induced with Phenobarbital intraperitoneally and 13-Naphtoflavone orally
- Test concentrations with justification for top dose:
- 0.05 - 0.16 - 0.5 - 1.6 - 5 mg/plate
- Vehicle / solvent:
- DMSO
Controlsopen allclose all
- Untreated negative controls:
- no
- Negative solvent / vehicle controls:
- yes
- Remarks:
- DMSO
- True negative controls:
- no
- Positive controls:
- yes
- Positive control substance:
- other: ICR 191 acridine mutagen dihydrochloride
- Remarks:
- TA97a (without metabolic activation)
- Untreated negative controls:
- no
- Negative solvent / vehicle controls:
- yes
- Remarks:
- DMSO
- True negative controls:
- no
- Positive controls:
- yes
- Positive control substance:
- other: 4-Nitro-1,2-phenylenediamine
- Remarks:
- TA98 (without metabolic activation)
- Untreated negative controls:
- no
- Negative solvent / vehicle controls:
- yes
- Remarks:
- DMSO
- True negative controls:
- no
- Positive controls:
- yes
- Positive control substance:
- other: Nitrofurantoine
- Remarks:
- TA100 (without metabolic activation)
- Untreated negative controls:
- no
- Negative solvent / vehicle controls:
- yes
- True negative controls:
- no
- Positive controls:
- yes
- Positive control substance:
- sodium azide
- Remarks:
- TA1535 (wihtout metabolic activation)
- Untreated negative controls:
- no
- Negative solvent / vehicle controls:
- yes
- True negative controls:
- no
- Positive controls:
- yes
- Positive control substance:
- other: 2-Aminoanthraceen
- Remarks:
- TA 97a, TA 98, TA 100, TA 1535 (with metabolic activation)
- Untreated negative controls:
- no
- Negative solvent / vehicle controls:
- yes
- True negative controls:
- no
- Positive controls:
- yes
- Positive control substance:
- other: Cumene Hydroperoxide
- Remarks:
- TA102 (without metabolic activation)
- Untreated negative controls:
- no
- Negative solvent / vehicle controls:
- yes
- True negative controls:
- no
- Positive controls:
- yes
- Positive control substance:
- other: Danthron
- Remarks:
- TA102 (with metabolic activation)
- Details on test system and experimental conditions:
- METHOD OF APPLICATION: in agar (plate incorporation)
DURATION
- Exposure duration: 48 h at 37 °C (Plates of the mutagenicity test)
24 h (Plates for confirmation of the genotypes)
SELECTION AGENT: L-Histidin
NUMBER OF REPLICATIONS: 3
Titer of the overnight culture: > 1*10^8 cells/mL.
DETERMINATION OF CYTOTOXICITY
- Method: Background lawn
OTHER EXAMINATIONS:
- Other: Genotypes were evaluated for each study. - Evaluation criteria:
- Since a reduced background lawn is regarded to be a cytotoxic effect, plates with reduced background lawn were not included into evaluation procedures.
Arithmetic mean values and standard deviations were calculated from colonies per plate of three replicates. For evaluation of the results the induction rate of the mean values was calculated :
Induction rate: revertant colonies of test item/revertant colonies of the corresponding control
The test item is to be interpretated mutagenic if there is a concentration effect relationship and the induction rate is >=2. The data presented in the tables are computer generated and rounded for presentation. Thus manual calculation of results based an the data in this report may yield minor deviations from these figures.
Results and discussion
Test resultsopen allclose all
- Key result
- Species / strain:
- S. typhimurium TA 1535
- Metabolic activation:
- with and without
- Genotoxicity:
- negative
- Cytotoxicity / choice of top concentrations:
- no cytotoxicity
- Vehicle controls validity:
- valid
- Untreated negative controls validity:
- not examined
- Positive controls validity:
- valid
- Key result
- Species / strain:
- S. typhimurium, other: TA97a
- Metabolic activation:
- with and without
- Genotoxicity:
- negative
- Cytotoxicity / choice of top concentrations:
- cytotoxicity
- Vehicle controls validity:
- valid
- Untreated negative controls validity:
- not examined
- Positive controls validity:
- valid
- Key result
- Species / strain:
- S. typhimurium TA 98
- Metabolic activation:
- with and without
- Genotoxicity:
- negative
- Cytotoxicity / choice of top concentrations:
- no cytotoxicity
- Vehicle controls validity:
- valid
- Untreated negative controls validity:
- not examined
- Positive controls validity:
- valid
- Key result
- Species / strain:
- S. typhimurium TA 100
- Metabolic activation:
- with and without
- Genotoxicity:
- negative
- Cytotoxicity / choice of top concentrations:
- no cytotoxicity
- Vehicle controls validity:
- valid
- Untreated negative controls validity:
- not examined
- Positive controls validity:
- valid
- Key result
- Species / strain:
- S. typhimurium TA 102
- Metabolic activation:
- with and without
- Genotoxicity:
- negative
- Cytotoxicity / choice of top concentrations:
- no cytotoxicity
- Vehicle controls validity:
- valid
- Untreated negative controls validity:
- not examined
- Positive controls validity:
- valid
- Additional information on results:
- ADDITIONAL INFORMATION ON CYTOTOXICITY: TA97a showed at a concentration of 5 mg/plate with and without metabolic activation cytotoxic effects. The other strains showed no cytotoxic effects.
Any other information on results incl. tables
|
Concentration |
S9 |
TA97a |
TA98 |
TA100 |
TA102 |
TA 1535 |
|
5 |
- |
n.a. |
22 |
109 |
93 |
37 |
|
1.6 |
- |
334 |
24 |
157 |
307 |
39 |
|
0.5 |
- |
311 |
33 |
162 |
294 |
32 |
|
0.16 |
- |
355 |
21 |
155 |
357 |
29 |
|
0.05 |
- |
407 |
31 |
131 |
307 |
27 |
2nd test |
5 |
- |
173 |
31 |
77 |
258 |
20 |
|
1.6 |
- |
325 |
29 |
152 |
463 |
26 |
|
0.5 |
- |
422 |
32 |
125 |
486 |
19 |
|
0.16 |
- |
388 |
32 |
142 |
484 |
19 |
|
0.05 |
- |
415 |
34 |
158 |
554 |
19 |
|
5 |
+ |
n.a. |
13 |
94 |
298 |
21 |
|
1.6 |
+ |
292 |
22 |
100 |
300 |
13 |
|
0.5 |
+ |
341 |
21 |
98 |
287 |
14 |
|
0.16 |
+ |
329 |
32 |
80 |
251 |
14 |
|
0.05 |
+ |
349 |
23 |
79 |
241 |
12 |
2nd test |
5 |
+ |
n.a. |
34 |
102 |
439 |
11 |
|
1.6 |
+ |
306 |
45 |
131 |
539 |
11 |
|
0.5 |
+ |
326 |
36 |
118 |
647 |
10 |
|
0.16 |
+ |
340 |
45 |
129 |
639 |
16 |
|
0.05 |
+ |
434 |
38 |
107 |
708 |
14 |
ICR |
- |
>3.1 (1st test), |
|
|
|
|
|
2-AA |
+ |
>3.8 (1st test), |
>45.3 (1st test), |
>11.8 (1st test), |
|
9.8 (1st test), |
|
|
4-nitro-1,2-phenylenediamine |
- |
|
6.0 (1st test), |
|
|
|
|
Nitrofurantoine |
- |
|
|
3.5 (1st test), |
|
|
|
Cumene hydroperoxide |
- |
|
|
|
>3.7 (1st test), |
|
Danthron |
+ |
|
|
|
>2.7 (1st test), |
|
|
Sodium azide |
- |
|
|
|
|
9.0 (1st test), |
Applicant's summary and conclusion
- Conclusions:
- In this study the test item was found to have no mutagenic effects an Salmonella typhimurium strains TA 97 a, TA 98, TA 100, TA 102 and TA 1535 with (+) and without (-) the metabolic activation system S9 from male Wistar rats at concentrations up to 5 mg/plate.
- Executive summary:
The mutagenic effects of the test item were determined in a reverse mutation assay according to the principles of OECD Guideline No. 471 and EEC Directive 2000/32/EEC Method B. 13/14. Test systems were the Salmonelle typhimurium strains TA 97a, TA 98, TA 100, TA 102 and TA 1535 with (+) and without (-) the metabolic activation system S9 (from male Wistar rats) each. Positive and negative controls were included in each study. Duration of each study was 48 h. The test item was dissolved in DMSO and applied once at test initiation with the concentrations 0.05 - 0.16 - 0.5 - 1.6 - 5 mg/plate. The test substance showed no mutagenic effects with and without metabolic activation. Cytotoxic effects were observed only for TA97a in the highest concentration of 5 mg/plate.
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