Cyclohex-3-ene-1-carbaldehyde

Short-term toxicity to fish

Link to relevant study record(s)

Reference

Reference 1

Endpoint:

short-term toxicity to fish

Type of information:

experimental study

Adequacy of study:

weight of evidence

Reliability:

3 (not reliable)

Rationale for reliability incl. deficiencies:

significant methodological deficiencies

Remarks:

Test procedure in accordance with national standard methods with acceptable restrictions. The described test is only used for weight of evidence, since the oxygen content of the test medium during the test period was too low.

Qualifier:

equivalent or similar to guideline

Guideline:

OECD Guideline 204 (Fish, Prolonged Toxicity Test: 14-day Study)

Deviations:

no

GLP compliance:

not specified

Details on sampling:

Oxygen content, pH and concentrations of the test compound were determined four times immediately before, and four times immediately after renewal of the solution, for two concentrations of each compound.

Vehicle:

yes

Details on test solutions:

Carrier 72 µg/L acetone

Test organisms (species):

Poecilia reticulata

Details on test organisms:

Fish (P. reticulata) were laboratory-reared, and acclimated to the watet used in the tests (Alabaster and Abrams, 1964) for at least 12 days prior to the experiments. They were 2-3 months old at the start of the experiment. Ten fish were exposed to each concentration. Control fish were exposed to 72 µL/L acetone, which was used as a carrier solvent for most aldehydes. The room in which the experiments were carried out was illuminated for 12 hours per day, and kept at a temperature of 21-23 °C. Fish were fed a commercial food (TetraPhyll, Tetra Werke, Germany) daily.

Test type:

semi-static

Water media type:

freshwater

Total exposure duration:

14 d

Test temperature:

21 - 23 °C

pH:

range 6.5 - 7.5

Dissolved oxygen:

Oxygen content was sometimes found to be very low, approx. 3 mg/L, 24 h after preparation of the solutions.

Duration:

14 d

Dose descriptor:

LC50

Effect conc.:

10.2 µmol/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Duration:

14 d

Dose descriptor:

LC50

Effect conc.:

1.1 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Reference 2

Endpoint:

short-term toxicity to fish

Type of information:

experimental study

Adequacy of study:

weight of evidence

Study period:

1983-01-03 till 1983-01-06

Reliability:

4 (not assignable)

Rationale for reliability incl. deficiencies:

documentation insufficient for assessment

Qualifier:

no guideline followed

Principles of method if other than guideline:

no data on guideline

GLP compliance:

no

Analytical monitoring:

not specified

Vehicle:

yes

Details on test solutions:

5 g substance with 5 g Ethanol pre-dissolved and filled up to 500 mL

Test organisms (species):

Danio rerio (previous name: Brachydanio rerio)

Details on test organisms:

no data

Test type:

static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

96 h

Test temperature:

16.5 - 20.8 °C

pH:

4.9 - 7.1

Dissolved oxygen:

8.8 - 10.4 mg/L O2

Nominal and measured concentrations:

Nominal concentration: 10 -100 mg/L

Reference substance (positive control):

not required

Duration:

96 h

Dose descriptor:

LC0

Effect conc.:

30 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Key result

Duration:

96 h

Dose descriptor:

LC50

Effect conc.:

34 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Duration:

96 h

Dose descriptor:

LC100

Effect conc.:

38 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Conclusions:

The acute toxicity of cyclohex-3 -ene-1 -carbaldehyde to fish (Danio rerio) was observed during 96 hours exposure at LC50 of 34 mg/L.

Executive summary:

The acute toxicity of cyclohex-3 -ene-1 -carbaldehyde to fish (Danio rerio) was observed during 96 hours exposure at LC50 of 34 mg/L.

Description of key information

The acute toxicity of cyclohex-3 -ene-1 -carbaldehyde to fish was investigated in two acute toxicity studies (Bayer 1983, Deneer 1988).

 

Bayer 1983:

The study is expected to have been conducted according standard procedures at that time, but the documentation is lacking details. In this study the fish (Danio rerio) were observed during 96 hours exposure at different concentrations, which were determined in pre tests. Temperature, oxygen content and pH were monitored and noted every 24h.

The LC₅₀ was determined via probit analysis to be 34 mg/L.

 

Deneer 1988:

The study was performed according OECD 204 (Fish: prolonged toxicity test: 14d study). However, the study was determined to be unreliable, as the oxygen content was sometimes found to be very low, approx. 3 mg/L, 24 h after preparation of the solutions.

The effects of cyclohex-3-ene-1-carbaldehyde on the fish (P. reticulata) were nonetheless recorded for test vessels, which were not affected from low oxygen content. A 14d-LC₅₀ of 1.1 mg/L was determined.

 

The available studies for cyclohex-3-ene-1-carbaldehyde provide an approximation of the aquatic toxicty of the substance. The LC₅₀ to the investigated fish lies between 1.1 mg/L (Deneer 1988) and 34 mg/L (Bayer 1983) and the lower value is chosen as key value as a worst case. However, the substance is registered in the tonnageband art. 18 1 - 1000 t/a and therefore no further assessment is stipulated.

Key value for chemical safety assessment

Fresh water fish

Fresh water fish

Dose descriptor:

LC50

Effect concentration:

1.1 mg/L