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EC number: 284-392-0 | CAS number: 84864-68-6
Biodegradation in water: screening tests
Administrative data
- Endpoint:
- biodegradation in water: inherent biodegradability
- Type of information:
- experimental study
- Adequacy of study:
- weight of evidence
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- other: Data is from peer reviewed publication
Data source
Reference
- Reference Type:
- publication
- Title:
- The Degradation Of Dyestuffs: Part II Behaviour Of Dyestuffs In Aerobic Biodegradation Tests
- Author:
- U. Pagga and O. Brown
- Year:
- 1 986
- Bibliographic source:
- Chemosphere, Vo1.15, No.4, pp 479-491, 1986
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 302 B (Inherent biodegradability: Zahn-Wellens/EMPA Test)
- Version / remarks:
- Modified method
- Principles of method if other than guideline:
- Short term aerobic biodegradation study was conducted for the test material Acid yellow 3.
- GLP compliance:
- not specified
Test material
- Reference substance name:
- 8004-92-0
- Cas Number:
- 8004-92-0
- IUPAC Name:
- 8004-92-0
- Reference substance name:
- C.I. Food Yellow 13
- IUPAC Name:
- C.I. Food Yellow 13
- Reference substance name:
- 616-849-0
- EC Number:
- 616-849-0
- IUPAC Name:
- 616-849-0
- Details on test material:
- - Name of test material (as cited in study report): Acid yellow 3
- Molecular formula (if other than submission substance): C18-H9-N-O8-S2.2Na
- Molecular weight (if other than submission substance): 477.3801 g/mol
- Smiles notation (if other than submission substance): c1ccc2c(c1)C(=O)C(C2=O)c3ccc4cc(cc(c4n3)S(=O)(=O)[O-])S(=O)(=O)[O-].[Na+].[Na+]
- InChl (if other than submission substance): 1S/C18H11NO8S2.2Na/c20-17-11-3-1-2-4-12(11)18(21)15(17)13-6-5-9-7-10(28(22,23)24)8-14(16(9)19-13)29(25,26)27;;/h1-8,15H,(H,22,23,24)(H,25,26,27);;/q;2*+1/p-2
- Structural formula attached as image file (if other than submission substance): see Fig.
- Substance type: Organic
- Physical state: No data available
- Analytical purity: No data available
- Impurities (identity and concentrations): No data available
- Composition of test material, percentage of components: No data available
- Isomers composition: No data available
- Purity test date: No data available
- Lot/batch No.: No data available
- Expiration date of the lot/batch: No data available
- Radiochemical purity (if radiolabelling): No data available
- Specific activity (if radiolabelling): No data available
- Locations of the label (if radiolabelling): No data available
- Expiration date of radiochemical substance (if radiolabelling): No data available
- Stability under test conditions: No data available
- Storage condition of test material: No data available
- Other: No data available
Constituent 1
Constituent 2
Constituent 3
Study design
- Oxygen conditions:
- aerobic
- Inoculum or test system:
- activated sludge, adapted
- Details on inoculum:
- - Source of inoculum/activated sludge (e.g. location, sampling depth, contamination history, procedure): treatment plants conveniently located to the laboratories carrying out the test. These treatment plants received communal and/or industrial wastewater.
- Laboratory culture: No data available
- Method of cultivation: No data available
- Storage conditions: No data available
- Storage length: No data available
- Preparation of inoculum for exposure: No data available
- Pretreatment: No data available
- Concentration of sludge: 0.5 g/L dry material
- Initial cell/biomass concentration: No data available
- Water filtered: No data available
- Type and size of filter used, if any: No data available - Duration of test (contact time):
- 42 d
Initial test substance concentration
- Initial conc.:
- 100 mg/L
- Based on:
- DOC
Parameter followed for biodegradation estimationopen allclose all
- Parameter followed for biodegradation estimation:
- DOC removal
- Parameter followed for biodegradation estimation:
- other: Color removal
- Details on study design:
- No data available
Results and discussion
% Degradation
- Parameter:
- % degradation (DOC removal)
- Sampling time:
- 42 d
- Remarks on result:
- other: -44 %
Any other information on results incl. tables
No color elimination was noted in 42 days study period
Applicant's summary and conclusion
- Interpretation of results:
- under test conditions no biodegradation observed
- Conclusions:
- The test compound Acid yellow 3 showed -44% DOC removal suggesting that it is not biodegradable under the mentioned test conditions.
- Executive summary:
- Short term aerobic biodegradation study
was conducted for the test material Acid yellow 3.
Readily water-soluble dyestuff acid yellow 3 was subjected to modification of the OECD Guideline 302B of the static test method. 100 mg/L dyestuff was tested with 0.5 mg/L activated sludge for inherent biodegradtion for upto 42 days. The feeding used for inoculums was 100 mg/L yeast extract every week.
Extinction at absorption maximum and DOC (dissolved organic carbon) were the parameters included in the study.
The test compound Acid yellow 3 showed -44% DOC removal suggesting that it is not biodegradable under the mentioned test conditions.
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