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Environmental fate & pathways

Biodegradation in water: screening tests

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biodegradation in water: inherent biodegradability
Type of information:
experimental study
Adequacy of study:
weight of evidence
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
other: Data is from peer reviewed publication

Data source

Reference Type:
The Degradation Of Dyestuffs: Part II Behaviour Of Dyestuffs In Aerobic Biodegradation Tests
U. Pagga and O. Brown
Bibliographic source:
Chemosphere, Vo1.15, No.4, pp 479-491, 1986

Materials and methods

Test guideline
according to guideline
OECD Guideline 302 B (Inherent biodegradability: Zahn-Wellens/EMPA Test)
Version / remarks:
Modified method
Principles of method if other than guideline:
Short term aerobic biodegradation study was conducted for the test material Acid yellow 3.
GLP compliance:
not specified

Test material

Constituent 1
Reference substance name:
Cas Number:
Constituent 2
Reference substance name:
C.I. Food Yellow 13
C.I. Food Yellow 13
Constituent 3
Reference substance name:
EC Number:
Details on test material:
- Name of test material (as cited in study report): Acid yellow 3
- Molecular formula (if other than submission substance): C18-H9-N-O8-S2.2Na
- Molecular weight (if other than submission substance): 477.3801 g/mol
- Smiles notation (if other than submission substance): c1ccc2c(c1)C(=O)C(C2=O)c3ccc4cc(cc(c4n3)S(=O)(=O)[O-])S(=O)(=O)[O-].[Na+].[Na+]
- InChl (if other than submission substance): 1S/C18H11NO8S2.2Na/c20-17-11-3-1-2-4-12(11)18(21)15(17)13-6-5-9-7-10(28(22,23)24)8-14(16(9)19-13)29(25,26)27;;/h1-8,15H,(H,22,23,24)(H,25,26,27);;/q;2*+1/p-2
- Structural formula attached as image file (if other than submission substance): see Fig.
- Substance type: Organic
- Physical state: No data available
- Analytical purity: No data available
- Impurities (identity and concentrations): No data available
- Composition of test material, percentage of components: No data available
- Isomers composition: No data available
- Purity test date: No data available
- Lot/batch No.: No data available
- Expiration date of the lot/batch: No data available
- Radiochemical purity (if radiolabelling): No data available
- Specific activity (if radiolabelling): No data available
- Locations of the label (if radiolabelling): No data available
- Expiration date of radiochemical substance (if radiolabelling): No data available
- Stability under test conditions: No data available
- Storage condition of test material: No data available
- Other: No data available

Study design

Oxygen conditions:
Inoculum or test system:
activated sludge, adapted
Details on inoculum:
- Source of inoculum/activated sludge (e.g. location, sampling depth, contamination history, procedure): treatment plants conveniently located to the laboratories carrying out the test. These treatment plants received communal and/or industrial wastewater.
- Laboratory culture: No data available
- Method of cultivation: No data available
- Storage conditions: No data available
- Storage length: No data available
- Preparation of inoculum for exposure: No data available
- Pretreatment: No data available
- Concentration of sludge: 0.5 g/L dry material
- Initial cell/biomass concentration: No data available
- Water filtered: No data available
- Type and size of filter used, if any: No data available
Duration of test (contact time):
42 d
Initial test substance concentration
Initial conc.:
100 mg/L
Based on:
Parameter followed for biodegradation estimationopen allclose all
Parameter followed for biodegradation estimation:
DOC removal
Parameter followed for biodegradation estimation:
other: Color removal
Details on study design:
No data available

Results and discussion

% Degradation
% degradation (DOC removal)
Sampling time:
42 d
Remarks on result:
other: -44 %

Any other information on results incl. tables

No color elimination was noted in 42 days study period

Applicant's summary and conclusion

Interpretation of results:
under test conditions no biodegradation observed
The test compound Acid yellow 3 showed -44% DOC removal suggesting that it is not biodegradable under the mentioned test conditions.
Executive summary:
Short term aerobic biodegradation study was conducted for the test material Acid yellow 3.


Readily water-soluble dyestuff acid yellow 3 was subjected to modification of the OECD Guideline 302B of the static test method. 100 mg/L dyestuff was tested with 0.5 mg/L activated sludge for inherent biodegradtion for upto 42 days. The feeding used for inoculums was 100 mg/L yeast extract every week.


Extinction at absorption maximum and DOC (dissolved organic carbon) were the parameters included in the study.


The test compound Acid yellow 3 showed -44% DOC removal suggesting that it is not biodegradable under the mentioned test conditions.