Sodium 2-(2,3-dihydro-1,3-dioxo-1H-inden-2-yl)quinoline-6-sulphonate

Short-term toxicity to fish

Link to relevant study record(s)

Reference

Endpoint:

short-term toxicity to fish

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

guideline study

Justification for type of information:

Data is from UERL lab

Qualifier:

according to guideline

Guideline:

OECD Guideline 203 (Fish, Acute Toxicity Test)

Principles of method if other than guideline:

This study was designed to assess the toxic effects of the test compound on the Zebra Fish Danio rerio .Bowl aquaria containing 2 liters of potable water (passed through reverse osmosis system) were loaded with 8 fishes. A static procedure was used for the study and it was conducted in compliance with the OECD guideline 203.

GLP compliance:

yes

Analytical monitoring:

not specified

Details on sampling:

- Sample storage conditions before analysis: Ambient Temp (23 to 27 °C)

Vehicle:

yes

Test organisms (species):

Danio rerio (previous name: Brachydanio rerio)

Details on test organisms:

TEST ORGANISM
- Common name:Zebra Fish
- Strain:Danio rerio
- Source: Unique Aqua Fish Corner
- Age at study initiation (mean and range, SD):3-4 months
- Length at study initiation (length definition, mean, range and SD): average 3.42 cm
- Weight at study initiation (mean and range, SD): average 0.335 g
- Method of breeding:
- Feeding during test
- Food type:Standard brand feed
- Frequency: once daily

Test type:

static

Water media type:

freshwater

Limit test:

yes

Total exposure duration:

96 h

Hardness:

148.5

Test temperature:

25°C

pH:

7.01

Dissolved oxygen:

7.06 (mg/l)

Nominal and measured concentrations:

100 mg/L

Details on test conditions:

TEST SYSTEM
- Test vessel: all plastic aquaria having the water capacity of 5 liters
- Type (delete if not applicable): No data available
- Material, size, headspace, fill volume: No data available
- Aeration: No data available
- Type of flow-through (e.g. peristaltic or proportional diluter): No data available
- Renewal rate of test solution (frequency/flow rate): No data available
- No. of organisms per vessel: 8 fishes
- No. of vessels per concentration (replicates): No data available
- No. of vessels per control (replicates): 8 fishes
- No. of vessels per vehicle control (replicates): No data available
- Biomass loading rate: No data available

TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: No data available
- Total organic carbon: No data available
- Particulate matter: No data available
- Metals: No data available
- Pesticides: No data available
- Chlorine: No data available
- Alkalinity: No data available
- Ca/mg ratio: No data available
- Conductivity: No data available
- Culture medium different from test medium: No data available
- Intervals of water quality measurement: No data available

OTHER TEST CONDITIONS
- Adjustment of pH: No data available
- Photoperiod: 12 hours light and 12-hour darkness
- Light intensity: No data available

EFFECT PARAMETERS MEASURED (with observation intervals if applicable) : Mortality and visible symptoms

TEST CONCENTRATIONS
- Spacing factor for test concentrations: No data available
- Justification for using less concentrations than requested by guideline: No data available
- Range finding study: A Limit test was conducted with the test concentrations of 100 mg/L. Observations (mortality, visible symptoms, pH, Temperature, dissolved oxygen content) were recorded after 24, 48, 72 and 96 hours of the start of the experiment.
- Test concentrations: 100 mg/L
- Results used to determine the conditions for the definitive study:

Reference substance (positive control):

no

Key result

Duration:

96 h

Dose descriptor:

LC50

Effect conc.:

> 100 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Remarks on result:

other: Nontoxic

Details on results:

- Behavioural abnormalities: apathy, Narcotic-like state, Swimming near water surface, Slow movements compared to control, Surfacing behaviour, Loss of equilibrium, Swimming movement, Hyperactivity, Hypoactivity, Bending behavior, Fin rot, Invert Swimming, Swimming behaviour, Respiratory Function , Pigmentation
- Observations on body length and weight: No data available
- Other biological observations: No data available
- Mortality of control: No mortality was noted in the control fishes
- Other adverse effects control: No data available
- Abnormal responses: No data available
- Any observations (e.g. precipitation) that might cause a difference between measured and nominal values: No data available
- Effect concentrations exceeding solubility of substance in test medium: No data available

Validity criteria fulfilled:

yes

Conclusions:

Based on nominal concentrations, experimental median lethal Concentrations [LC-50 (96 h)] for CAS No. 29556-33-0on Zebra Fish Danio rerio was found to be >100mg/L.

Executive summary:

This study was designed to assess the toxic effects of the test compound on the Zebra Fish Danio rerio.Bowl aquaria containing 2 liters of potable water (passed through reverse osmosis system) were loaded with 8 fishes. A static procedure was used for the study and it was conducted in compliance with the OECD guideline 203. A 96 h study was conducted for CAS No.29556-33-0 on Zebra Fish Danio rerio, at a test concentrations of 100 mg/L. The fishes were moving slowly as compared to control. No mortalities were found in the control aquaria. The lethal concentrations LC50 was found to be >100mg/L.

Description of key information

A static procedure was used for the study and it was conducted in compliance with the OECD guideline 203. A 96 h study was conducted for CAS No.29556-33-0 on Zebra Fish Danio rerio, at a test concentrations of 100 mg/L. The lethal concentrations LC50 was determine to be >100mg/L.

Key value for chemical safety assessment

Fresh water fish

Fresh water fish

Effect concentration:

100 mg/L

Additional information

This study was designed to assess the toxic effects of the test compound on the Zebra FishDanio rerio.Bowl aquaria containing 2 liters of potable water (passed through reverse osmosis system) were loaded with 8 fishes. A static procedure was used for the study and it was conducted in compliance with the OECD guideline 203. A 96 h study was conducted for CAS No.29556-33-0 on Zebra Fish Danio rerio, at a test concentrations of 100 mg/L. The fishes were moving slowly as compared to control. No mortalities were found in the control aquaria. The lethal concentrations LC50 was determine to be >100mg/L.