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Administrative data

Endpoint:
eye irritation: in vivo
Type of information:
experimental study
Adequacy of study:
key study
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
other: Comparable to guideline study with acceptable restrictions.
Cross-reference
Reason / purpose for cross-reference:
reference to same study

Data source

Reference
Reference Type:
other company data
Title:
Unnamed
Year:
1969
Report date:
1969

Materials and methods

Test guideline
Qualifier:
equivalent or similar to guideline
Guideline:
OECD Guideline 405 (Acute Eye Irritation / Corrosion)
Principles of method if other than guideline:
Study was performed acc. to internal BASF method, which was in large part equivalent to OECD 405
Deviations from OECD 405: 2 test animals; eye examination 24 hrs before testing not mentioned
GLP compliance:
no

Test material

Constituent 1
Chemical structure
Reference substance name:
2,5,6-trimethylcyclohex-2-en-1-one
EC Number:
243-473-0
EC Name:
2,5,6-trimethylcyclohex-2-en-1-one
Cas Number:
20030-30-2
Molecular formula:
C9H14O
IUPAC Name:
2,5,6-trimethylcyclohex-2-en-1-one
Details on test material:
- Name of test material (as cited in study report): 2,5,6-Trimethyl-2-cyclohexen-1-on
- Analytical purity: 98 - 99%

Test animals / tissue source

Species:
rabbit
Strain:
Vienna White

Test system

Vehicle:
unchanged (no vehicle)
Controls:
other: untreated left eye of same animal
Amount / concentration applied:
TEST MATERIAL
- Amount(s) applied (volume or weight with unit): 0.05 ml
- Concentration (if solution): 100%
Observation period (in vivo):
8 days
Number of animals or in vitro replicates:
2
Details on study design:
REMOVAL OF TEST SUBSTANCE
- Washing (if done): no washing

SCORING SYSTEM:
Conjunctival redness, chemosis and corneal opacity were scored according to the below described system.
Although the results in the report were originally not given as Draize scores the data can be transferred into the Draize scoring system (in brackets). (+) = none - negligible effect (Draize score 0)
+ = slight effect (1)
++ = moderate effect (2)
+++ = severe effect (> = 3)

Results and discussion

In vivo

Results
Irritation parameter:
overall irritation score
Basis:
mean
Time point:
other: 24h
Score:
ca. 1
Max. score:
2
Reversibility:
fully reversible within: 8 days
Remarks on result:
other: only results at 24h, not 24-72h

Any other information on results incl. tables

Results:

Readings Animal cornea Iris conjunctiva Symptons
opacity redness swelling discharge
1h 1 1 0 2 3 0  
2 1 0 2 2 0  
24 h 1 1 0 2 1 0  
2 1 2 2 1 0  
8 d 1 0 0 0 0 0  
2 0 0 0 0 0  

Applicant's summary and conclusion

Interpretation of results:
not irritating
Remarks:
Migrated information Criteria used for interpretation of results: EU
Conclusions:
Due to resulted maximum erythema scoring = 2 at 24 h and the full reversiblity of all effects, no classification is necessary.

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