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EC number: 243-473-0 | CAS number: 20030-30-2
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- Ecotoxicological Summary
- Aquatic toxicity
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- Short-term toxicity to fish
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Genetic toxicity: in vitro
Administrative data
- Endpoint:
- in vitro gene mutation study in bacteria
- Remarks:
- Type of genotoxicity: gene mutation
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- other: Guideline study (OECD)
Data source
Reference
- Reference Type:
- other company data
- Title:
- Unnamed
- Year:
- 1 989
- Report date:
- 1989
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 471 (Bacterial Reverse Mutation Assay)
- GLP compliance:
- no
- Type of assay:
- bacterial reverse mutation assay
Test material
- Reference substance name:
- 2,5,6-trimethylcyclohex-2-en-1-one
- EC Number:
- 243-473-0
- EC Name:
- 2,5,6-trimethylcyclohex-2-en-1-one
- Cas Number:
- 20030-30-2
- Molecular formula:
- C9H14O
- IUPAC Name:
- 2,5,6-trimethylcyclohex-2-en-1-one
- Details on test material:
- - Name of test material (as cited in study report): 2,3,6-Trimethylcyclohexen-1-on
- ZST Test Substance No . : 87/818
- Analytical purity: ca. 96 %
- Storage condition of test material: room temperature
Constituent 1
Method
Species / strain
- Species / strain / cell type:
- S. typhimurium TA 1535, TA 1537, TA 98 and TA 100
- Metabolic activation:
- with and without
- Metabolic activation system:
- Aroclor 1254-induced rat liver S9 mix
- Test concentrations with justification for top dose:
- Standard plate test (SPT): 100, 500, 2500 and 5000 ug/plate
Preincubation test (PIT): 100, 500 and 2500 µg/plate - Vehicle / solvent:
- - Vehicle(s)/solvent(s) used: DMSO
Controlsopen allclose all
- Untreated negative controls:
- yes
- Negative solvent / vehicle controls:
- yes
- Positive controls:
- yes
- Positive control substance:
- other: 2-aminoanthracene, 10 µg/plate, dissolved in DMSO, for the strains TA100, TA98, TA1537 and TA1535
- Remarks:
- with metabolic activation
- Positive controls:
- yes
- Positive control substance:
- other: N-methyl-N-nitro-N-nitrosoguanidine; 5 µg/plate, dissolved in DMSO, for strains TA100 and TA1535
- Remarks:
- without metabolic activation
- Positive controls:
- yes
- Positive control substance:
- other: 4-nitro-o-phenylendiamine, 10 µg/plate, dissolved in DMSO, for strain TA98
- Remarks:
- without metabolic activation
- Positive controls:
- yes
- Positive control substance:
- 9-aminoacridine
- Remarks:
- without metabolic activation
Migrated to IUCLID6: 100 µg/palte, dissolved in DMSO, for strain TA1537
- Details on test system and experimental conditions:
- METHOD OF APPLICATION:
The experimental procedures are based on the methods described by Ames, (1973, 1975), Yahagi et al. (1977) and Matsushima et al. (1980).
1ST EXPERIMENT: in agar (plate incorporation) (SPT)
DURATION
- Exposure duration: 48 - 72 h, 37°C
2ND EXPERIMENT: preincubation (PIT)
DURATION
- Preincubation period: 20 min, 37°C
- Exposure duration: 48 - 72 h, 37°C
NUMBER OF REPLICATIONS: triplicates; 3 test plates per dose or per control
DETERMINATION OF CYTOTOXICITY
- Method: decrease in the number of revertants, clearing or diminution of the background lawn (= reduced his- or trp- background growth), reduction in the titer - Evaluation criteria:
- In general, a substance to be characterized as positive in the Ames test has to fulfill the following requirements:
- doubling of the spontaneous mutation rate (control)
- dose-response relationship
- reproducibility of the results
Results and discussion
Test results
- Species / strain:
- S. typhimurium TA 1535, TA 1537, TA 98 and TA 100
- Metabolic activation:
- with and without
- Genotoxicity:
- negative
- Cytotoxicity / choice of top concentrations:
- cytotoxicity
- Remarks:
- depending on the strain and test conditions at doses > 2500 µg/plate .
- Vehicle controls validity:
- valid
- Positive controls validity:
- valid
- Remarks on result:
- other: all strains/cell types tested
- Remarks:
- Migrated from field 'Test system'.
Any other information on results incl. tables
Results of SPT:
Strain | metabolic activation | Experiment | controls | µg substance / plate | Evaluation | |||||
solvent | positive | 20 | 100 | 500 | 2500 | 5000 | ||||
TA 1535 | - | 1 | 17 | 2100 | 15 | 17 | 16 | 11 | 11 | - |
- | 2 | 12 | 2150 | 22 | 18 | 23 | 13 | 8 | - | |
- | 3 | 14 | 1950 | 20 | 14 | 10 | 18 | 7 | - | |
+ | 1 | 21 | 123 | 21 | 23 | 19 | 19 | 7 | - | |
+ | 2 | 19 | 159 | 17 | 18 | 20 | 20 | 8 | - | |
+ | 3 | 19 | 154 | 22 | 18 | 23 | 16 | 13 | - | |
TA 1537 | - | 1 | 7 | 245 | 8 | 6 | 11 | 8 | B | - |
- | 2 | 8 | 372 | 5 | 7 | 7 | 6 | B | - | |
- | 3 | 8 | 367 | 8 | 10 | 8 | 9 | B | - | |
+ | 1 | 12 | 129 | 6 | 7 | 9 | 7 | 7 | - | |
+ | 2 | 9 | 117 | 9 | 14 | 11 | 9 | 8 | - | |
+ | 3 | 8 | 142 | 8 | 8 | 8 | 9 | 4 | - | |
TA 98 | - | 1 | 21 | 575 | 20 | 21 | 23 | 22 | 13B | - |
- | 2 | 22 | 647 | 19 | 23 | 26 | 21 | 10B | - | |
- | 3 | 24 | 652 | 26 | 18 | 20 | 16 | 13B | - | |
+ | 1 | 33 | 1310 | 42 | 33 | 36 | 32 | 28 | - | |
+ | 2 | 32 | 1180 | 31 | 49 | 38 | 36 | 28 | - | |
+ | 3 | 35 | 1060 | 31 | 35 | 41 | 38 | 33 | - | |
TA 100 | - | 1 | 101 | 1820 | 106 | 97 | 120 | 98 | 60B | - |
- | 2 | 97 | 1920 | 97 | 101 | 118 | 101 | 71B | - | |
- | 3 | 102 | 1950 | 120 | 98 | 101 | 91 | 70B | - | |
+ | 1 | 121 | 1890 | 135 | 122 | 111 | 102 | 97 | - | |
+ | 2 | 128 | 1830 | 105 | 133 | 103 | 101 | 84 | - | |
+ | 3 | 115 | 1870 | 119 | 133 | 128 | 114 | 88 | - |
B: reduced his-backround
Results of PIT:
mean number of revertant colonies / plate | ||||||||||
Strain | metabolic activation | Experiment | controls | µg substance / plate | Evaluation | |||||
solvent | positive | 4 | 20 | 100 | 500 | 2500 | ||||
TA 1535 | - | 1 | 19 | 1050 | 21 | 17 | 13 | 18 | 13 | - |
- | 2 | 12 | 1080 | 21 | 15 | 13 | 16 | 13 | - | |
- | 3 | 12 | 1100 | 13 | 15 | 13 | 14 | 13 | - | |
+ | 1 | 13 | 139 | 15 | 17 | 18 | 12 | 13 | - | |
+ | 2 | 16 | 132 | 19 | 18 | 20 | 12 | 13 | - | |
+ | 3 | 18 | 110 | 22 | 19 | 17 | 14 | 13 | - | |
TA 1537 | - | 1 | 8 | 423 | 7 | 11 | 10 | 5 | B | - |
- | 2 | 8 | 413 | 9 | 7 | 8 | 3 | B | - | |
- | 3 | 7 | 377 | 9 | 10 | 7 | 5 | B | - | |
+ | 1 | 13 | 129 | 9 | 8 | 7 | 8 | B | - | |
+ | 2 | 12 | 100 | 9 | 8 | 9 | 10 | B | - | |
+ | 3 | 11 | 85 | 9 | 8 | 10 | 11 | B | - | |
TA 98 | - | 1 | 19 | 985 | 24 | 26 | 26 | 23 | B | - |
- | 2 | 22 | 1050 | 26 | 23 | 21 | 13 | B | - | |
- | 3 | 20 | 1330 | 24 | 21 | 20 | 9 | B | - | |
+ | 1 | 32 | 878 | 30 | 41 | 33 | 33 | B | - | |
+ | 2 | 36 | 810 | 34 | 30 | 30 | 34 | B | - | |
+ | 3 | 34 | 700 | 37 | 34 | 40 | 36 | B | - | |
TA 100 | - | 1 | 106 | 1160 | 128 | 115 | 110 | 93 | B | - |
- | 2 | 106 | 1170 | 107 | 127 | 86 | 89 | B | - | |
- | 3 | 118 | 1100 | 106 | 107 | 103 | 112 | B | - | |
+ | 1 | 112 | 1060 | 123 | 98 | 118 | 128 | B | - | |
+ | 2 | 128 | 1150 | 104 | 106 | 107 | 134 | B | - | |
+ | 3 | 112 | 920 | 115 | 126 | 133 | 124 | B | - |
B: reduced his-backround
Applicant's summary and conclusion
- Conclusions:
- Interpretation of results (migrated information):
negative
According to the results of the present study, the test substance 2,3,6-Trimethylcyclohexen- 1-on is not mutagenic in the Ames test under the experimental conditions chosen above.
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