Registration Dossier

Diss Factsheets

Ecotoxicological information

Short-term toxicity to aquatic invertebrates

Currently viewing:

Administrative data

Link to relevant study record(s)

Reference
Endpoint:
short-term toxicity to aquatic invertebrates
Type of information:
experimental study
Adequacy of study:
key study
Study period:
22 August 1990 - 3 September 1991
Reliability:
1 (reliable without restriction)
Rationale for reliability incl. deficiencies:
guideline study
Qualifier:
according to guideline
Guideline:
OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)
Qualifier:
according to guideline
Guideline:
EPA OPP 72-2 (Aquatic Invertebrate Acute Toxicity Test)
GLP compliance:
yes
Analytical monitoring:
yes
Details on sampling:
- The sample bottles were shaken and aliquots transferred by bulb pipette to 40 mL vials. The total volume in each vial was made up to 20 mL with dechlorinated water (carbon filtered) followed by the addition of 8.0 mL reducing solution (3% stannous chloride dihydrate in 10 M hydrochloric acid). The vials were sealed and heated in a water bath at 80°C for one hour. The vials were then shaken for 30 seconds and allowed to stand at room temperature for one hour to stabilise.
Details on test solutions:
PREPARATION AND APPLICATION OF TEST SOLUTION
- Method: direct dispersion in water. A stock solution of 50 mg/L was prepared by dissolving the test sample in test water with the aid of ultrasonic disruption. The test series were prepared by further dilution of this stock with test water.
- Controls: yes
- Evidence of undissolved material: no precipitate was observed during the preparation of the stock or test solutions
Test organisms (species):
Daphnia magna
Details on test organisms:
TEST ORGANISM
- Common name: water flea
- Strain: Straus
- Source: laboratory culture originating from a strain supplied by IRCHA, France
- Age at study initiation: < 24 h
- Method of breeding: cultures were kept at 20° +/- 2°C in glass vessels containing 2 L of dechlorinated and aged tap water, and fed daily with a mixture of fry fish food and a suspension of mixed algae

Test type:
static
Water media type:
freshwater
Limit test:
no
Total exposure duration:
48 h
Hardness:
350 mgCaCO3/L
Test temperature:
21°C
pH:
8.0
Dissolved oxygen:
8.6-8.9 mgO2/L
Nominal and measured concentrations:
Nominal concentrations: 0.00032, 0.00056, 0.001, 0.0018, 0.0032, 0.0056, 0.01, 0.018, 0.032, 0.056 and 0.1 mg a.i./L
Measured concentrations: 0.0012, 0.0012, 0.0026, 0.0017, 0.0072, 0.0055, 0.0094, 0.015, 0.032, 0.048 and 0.096 mg a.i./L
Details on test conditions:
TEST SYSTEM
- Test vessel: glass jars containing 200 mL test solution
- Aeration: no
- No. of organisms per vessel: 10
- No. of vessels per concentration (replicates): 1
- No. of vessels per control (replicates): 1
- Biomass loading rate: 1daphnid/20mL solution

TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: dechlorinated (with sodium thiosulphate) and aged laboratory tap water.

OTHER TEST CONDITIONS
- Photoperiod: 16h light; 8 h darkness

EFFECT PARAMETERS MEASURED : immobilisation

TEST CONCENTRATIONS
- Spacing factor for test concentrations: 1.7
Duration:
48 h
Dose descriptor:
EC50
Effect conc.:
0.048 mg/L
Nominal / measured:
meas. (arithm. mean)
Conc. based on:
act. ingr.
Basis for effect:
other: immobilisation
Remarks on result:
other: 0.034-0.068
Duration:
48 h
Dose descriptor:
NOEC
Effect conc.:
0.003 mg/L
Nominal / measured:
estimated
Conc. based on:
act. ingr.
Validity criteria fulfilled:
yes

Description of key information

EC50 (48h) = 0.048 mg a.i./L (Daphnia magna)

Key value for chemical safety assessment

Fresh water invertebrates

Fresh water invertebrates
Effect concentration:
0.048 mg/L

Additional information

The acute toxicity of zinc bis dimethyldithiocarbamate (CAS No. 137-30-4) to Daphnia magna was investigated by Douglas et al. (1991). This test was conducted according to OECD Guideline 202,Daphnia sp. Acute Immobilisation Test (1984) and EPA OPP 72-2 (Aquatic Invertebrate Acute Toxicity Test), under GLP conditions. After 48 hours of exposure to the test substance within a semi-static water regime, daphnids immobilisation was reported, and a EC50 (48 h) of 0.048 mg a.i./L was determined.