Fatty acids, C8-10, oxybis(2-hydroxy-3,1-propanediyl) esters

Short-term toxicity to aquatic invertebrates

Link to relevant study record(s)

Reference

Endpoint:

short-term toxicity to aquatic invertebrates

Type of information:

experimental study

Adequacy of study:

key study

Study period:

17 Jul 2018 - 20 Jul 2018

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

guideline study

Qualifier:

according to guideline

Guideline:

OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)

Version / remarks:

adopted April 13, 2004

GLP compliance:

yes (incl. QA statement)

Remarks:

13. Mar 2017, Hess. Ministerium für Umwelt, Klimaschutz, Landwirtschaft und Verbraucherschutz, Wiesbaden, Germany

Analytical monitoring:

yes

Details on sampling:

- Concentrations: control and 100 mg/L loading rate
- Sampling method: samples of the freshly prepared test medium and at the end of the test (after 48 h) were taken.
- Sample storage conditions before analysis: stored in a refrigerator (4 ± 4 °C)

Vehicle:

no

Details on test solutions:

PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances)
- Method: A defined amount of the test item was added directly to the test water for the only loading rate of nominal 100 mg/L and was carefully stirred for 24 hours in the dark to dissolve as much of the test item as possible. The test medium was prepared by mixing 102.3 mg test item into 1023 mL test water. Then, the undissolved test item was separated by filtration (0.45 μm cellulose acetate filter) and the filtrate was used as test medium.

Test organisms (species):

Daphnia magna

Details on test organisms:

TEST ORGANISM
- Common name: water flea
- Strain/clone: clone 5
- Source: The daphnids introduced in the test were taken in-house culture.
- Age of test animals: 5.25 to 21.5 hours old

ACCLIMATION
- Acclimation period: none, breeding and test conditions were similar.
- Type and amount of food during breeding: green algae (Desmodesmus subspicatus)
- Feeding frequency during breeding: on all working days

Test type:

static

Water media type:

freshwater

Limit test:

yes

Total exposure duration:

48 h

Hardness:

250 mg/L as CaCO3

Test temperature:

Control: 20.4 - 20.7 °C
Test concentration: 21 - 20.7 °C

pH:

Control: 7.9 - 8.4
Test concentration: 7.7 - 7.8

Dissolved oxygen:

Control: 8.6 - 9.4 mg/L
Test concentration: 5.1 - 8.3 mg/L

Nominal and measured concentrations:

Nominal loading rate: 0 (Control), 100 mg/L (WAF)

Details on test conditions:

TEST SYSTEM
- Test vessel: glass beakers (100 mL)
- Type: covered with a lid
- Fill volume: 60 mL
- Aeration: none
- No. of organisms per vessel: 20
- No. of vessels per concentration (replicates): 4
- No. of vessels per control (replicates): 4

TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: Reconstituted Water (Elendt "M4")
- Alkalinity: 0.9 mmol/L

OTHER TEST CONDITIONS
- Adjustment of pH: no
- Photoperiod: 16 h light - 8 h dark
- Light intensity: 400 to 840 lux

EFFECT PARAMETERS MEASURED (with observation intervals if applicable) :
Mobility of the daphnids after 24 and 48 hours.

Reference substance (positive control):

yes

Remarks:

Potassium dichromate

Duration:

48 h

Dose descriptor:

EL50

Effect conc.:

> 100 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Remarks:

WAF (nominal loading)

Basis for effect:

mobility

Duration:

48 h

Dose descriptor:

NOELR

Effect conc.:

>= 100 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Remarks:

WAF (nominal loading)

Basis for effect:

mobility

Details on results:

The exposure concentration was considered stable over the test period based on TOC analyses (96% of initial TOC at 48 h). Therefore all reported results refer to nominal loading rates.
- Behavioural abnormalities: none
- Mortality of control: none
- Other adverse effects control: none

Results with reference substance (positive control):

Potassium dichromate: EC50 (48 h): 1.02 mg/L (Jan 2018)

Reported statistics and error estimates:

The 24-hour and 48-hour EL50, EL20 and EL10 and the 95 % confidence limits could not calculated statistically due to the absence of an effect and were determined directly from the raw data.
The NOEL and LOEL after 24 and 48 hours were determined directly from the raw data.

Table 1: Mobility of D. magna after 24 and 48 h

Nominal loading rate [mg test item/L]	No. of Daphnia magna tested	No. of immobilised Daphnia magna after		% of immobilised Daphnia magna after
		24 h	48 h	24 h	48 h
Control	20	0	0	0	0
100	20	0	0	0	0

 Table 2: Results for the Determination of the Carbon Content of the Test Item in the Test Samples

Sample description of nominal loading rate 100 mg test item/L	Corrected TOC* [mg/L]
freshly prepared	29.35
freshly prepared	29.44
After 48 h	28.06
After 48 h	28.18

*The results were corrected by the mean value of the control samples

Table 3: Validity criteria for OECD 202.

Criterion from the guideline	Outcome	Validity criterion fulfilled
In the control, including the control containing the solubilising agent, not more than 10% of the daphnids should have been immobilized.	0%	yes
The dissolved oxygen concentration at the end of the test should be ≥ 3 mg/L in control and test vessels.	5.1 - 8.3 mg/L	yes

 

Validity criteria fulfilled:

yes

Remarks:

For further details please refer to “Any other information on results incl. tables”.

Description of key information

EL50 (48 h) >100 mg/L (WAF, nominal loading rate), Daphnia magna (OECD 202)

Additional information

The acute toxicity of Fatty acids, C8-10, oxybis(2-hydroxy-3,1-propanediyl) esters (CAS 92044-91-2) to aquatic invertebrates was investigated in a static limit test according to OECD guideline 202 (GLP). The test organisms (Daphnia magna) were exposed to a water accommodated fraction (WAF) with a nominal loading rate of 100 mg/L for 48 hours and a control. The stability of the test substance in the test medium was analytically verified by TOC analysis. The recovery was 96% of initial TOC at 48 h, thus the test substance concentration was considered stable. At test termination after 48 h an EL50 (48 h) of >100 mg/L (NOELR ≥ 100 mg/L) based on the nominal loading rate (WAF) was determined.