Reaction mass of aluminium and magnesium oxide and spinel (Mg(AlO2)2)

Short-term toxicity to fish

Administrative data

Endpoint:

short-term toxicity to fish

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

other: GLP guideline study

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

2010

Report date:

2010

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

OECD Guideline 203 (Fish, Acute Toxicity Test)

GLP compliance:

yes (incl. QA statement)

Test material

Test material information

Constituent 1

Reference substance name:

dioxo-2,4-dioxa-1,5-dialumina-3-magnesapentane oxomagnesium alumane

EC Number:

701-289-2

Molecular formula:

Al, MgO, Mg(AlO2)2

IUPAC Name:

dioxo-2,4-dioxa-1,5-dialumina-3-magnesapentane oxomagnesium alumane

Details on test material:

Content: aluminium 708,000 mg/kg, magnesium 68,000 mg/kg, ammonium 22,000 mg/kg, and other ingredients
Batch number: Delivery of 21.04.2009
Expiry date: 2011-08-21

Sampling and analysis

Analytical monitoring:

yes

Details on sampling:

Examined at 0 and 96 hours.

Test solutions

Vehicle:

no

Details on test solutions:

A direct weighing was prepared to give the desired test concentration. 500.5 mg of the test item were added to 5 litres of dilution water (adjusted to pH 6), treated for one hour in an ultrasonic bath and stirred for 24 h on a magnetic stirrer. Undissolved particles of the test item were removed by filtration using a folded filter of the pore size 7-12 μm. The pH was measured to be 7.0 and was re-adjusted to 6.0.
The concentration of the test item was determined analytically by ICP-MS. Finally 7 fish were given to the test item concentration and the control.

Test organisms

Test organisms (species):

Danio rerio (previous name: Brachydanio rerio)

Details on test organisms:

Date supplied : 2009-10-13
Acclimatisation : Stock held since 2009-10-13 and acclimatised to the test conditions since then.
Feeding : Commercial fish food, daily. Discontinued 24 h prior to test.
Mortalities during acclimatisation period : < 5 %
Medication : none

Study design

Test type:

static

Water media type:

freshwater

Limit test:

yes

Total exposure duration:

96 h

Test conditions

Hardness:

dilution water: 239 mg/L CaCO3

Nominal and measured concentrations:

Nominal: 100 mg/L

Details on test conditions:

Experimental design : 1 test concentration plus 1 control, 7 fish per test concentration, no feeding during the exposure period

Reference substance (positive control):

not required

Results and discussion

Effect concentrations

Effect concentrations 1

Duration:

2 h

Dose descriptor:

LC50

Effect conc.:

> 100 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Effect concentrations 2

Duration:

24 h

Dose descriptor:

LC50

Effect conc.:

> 100 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Effect concentrations 3

Duration:

48 h

Dose descriptor:

LC50

Effect conc.:

> 100 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Effect concentrations 4

Duration:

72 h

Dose descriptor:

LC50

Effect conc.:

> 100 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Effect concentrations 5

Duration:

96 h

Dose descriptor:

LC50

Effect conc.:

> 100 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Details on results:

The LC50 (2h – 96h) are expressed in terms of actual loadings of Aluminium-Skimmings.

Any other information on results incl. tables

Sublethal observations / clinical signs:

Chemical analysis

Control	0h	96h
Aluminium determination (two replicates) [mg/L]	< 0.01 /   < 0.01	< 0.01 / <0.01
Aluminium determination (mean value) [mg/L]	< 0.01	< 0.01

Limit of quantification = 0.01 mg/L

100 mg/L	0h	96h
Aluminium determination (two replicates) [mg/L]	0.025 / 0.018	< 0.01 / <0.01
Aluminium determination (mean value) [mg/L]	0.022	< 0.01*

*value below the limit of quantification (0.01 mg/L); half the limit of quantification (0.005 mg/L) was used for further calculations.

Applicant's summary and conclusion

Executive summary:

A 96h-LC50 of >100 mg/l was determined with Danio rerio (Currenta, 2010).