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EC number: 240-815-0 | CAS number: 16752-77-5
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
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- Flash point
- Auto flammability
- Flammability
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- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
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- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
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- Endpoint summary
- Stability
- Biodegradation
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- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
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- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data

Toxicity to other aquatic organisms
Administrative data
- Endpoint:
- toxicity to other aquatic vertebrates
- Remarks:
- amphibian Xenopus laevis, larvae: (sub)lethal effects
- Type of information:
- experimental study
- Adequacy of study:
- supporting study
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 2 015
- Report date:
- 2015
Materials and methods
Test guideline
- Qualifier:
- equivalent or similar to guideline
- Guideline:
- other: U.S. EPA, Endocrine Disruptor Screening Program Test Guidelines, OPPTS 890.1100 Amphibian Metamorphosis (Frog) (October 2009)
- Deviations:
- no
- GLP compliance:
- yes
Test material
- Reference substance name:
- Methomyl
- EC Number:
- 240-815-0
- EC Name:
- Methomyl
- Cas Number:
- 16752-77-5
- Molecular formula:
- C5H10N2O2S
- IUPAC Name:
- (E)-[1-(methylsulfanyl)ethylidene]amino N-methylcarbamate
- Test material form:
- solid: crystalline
Constituent 1
- Specific details on test material used for the study:
- Methomyl technical
DPX-X1179-571
Purity: 99.4%
Sampling and analysis
- Analytical monitoring:
- yes
Test solutions
- Vehicle:
- yes
- Remarks:
- water
Test organisms
- Aquatic vertebrate type (other than fish):
- frog
- Test organisms (species):
- Xenopus laevis
Study design
- Test type:
- flow-through
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 21 d
Test conditions
- Hardness:
- 134-152 mg/L (as CaCO3)
- Test temperature:
- 21.9 to 22.4°C
- pH:
- 6.9 to 7.2
- Dissolved oxygen:
- 3.6 - 6.8 mg/L
- Nominal and measured concentrations:
- Nominal: 0, 18.3, 56.0, 168.0 μg/L
Measured: 0, 17.0, 52.2, 186 μg/L - Details on test conditions:
- The general experimental design entailed exposing tadpoles to 3 different concentrations of the test chemical (n = 4 replicates per concentration) and a dilution water control (n = 4 replicates). The primary endpoints evaluated were hind limb length, body length (snout to vent [SVL]), developmental stage, wet weight, thyroid histology, and daily mortality.
- Reference substance (positive control):
- no
Results and discussion
Effect concentrationsopen allclose all
- Key result
- Duration:
- 21 d
- Dose descriptor:
- NOEC
- Effect conc.:
- 186 µg/L
- Nominal / measured:
- meas. (TWA)
- Remarks on result:
- other: no effects at the highest dose tested
- Key result
- Duration:
- 21 d
- Dose descriptor:
- LOEC
- Effect conc.:
- > 186 µg/L
- Nominal / measured:
- meas. (TWA)
- Details on results:
- No statistically significant effects associated with any of the Amphibian Metamorphosis Assay endpoints were found during the present study. Mild histopathology of the thyroid glands was found in both the control and the test substance treatments and was determined by the certified pathologist to be unrelated to methomyl exposure. More specifically, the incidence rate of hypertrophy and hyperplasia was the highest in stage 61 (50% and 25%), lower in stage 62 and above (25% and 0%, 15% and 0% , and 10% and 0% at stages 62, 63, and 64, respectively), and the lowest in
Applicant's summary and conclusion
- Validity criteria fulfilled:
- yes
- Conclusions:
- 21d NOEC = 186 μg/L (the highest concentration tested)
21d LOEC > 186 μg/L - Executive summary:
The effect of the test substance on the metamorphosis of the South African clawed frog, (Xenopus laevis), was assessed in a flow-through, 21-day test initiated with Nieuwkoop and Faber (3) (NF) stage 51 larvae in accordance with test guidelines (U.S. EPA, Endocrine Disruptor Screening Program Test Guidelines, OPPTS 890.1100 Amphibian Metamorphosis (Frog) (October 2009)). Nominal concentrations tested were 0, 18.3, 56.0, 168.0 μg/L. Measured concentrations were 0, 17.0, 52.2, 186 μg/L. The general experimental design entailed exposing tadpoles to 3 different concentrations of the test chemical (n = 4 replicates per concentration) and a dilution water control (n = 4 replicates). The primary endpoints evaluated were hind limb length, body length (snout to vent [SVL]), developmental stage, wet weight, thyroid histology, and daily mortality.
The test substance did not significantly alter 7-day hind-limb length (HLL) or 21-day HLL. No effect on median (NF) developmental stage was observed at 7 days or 21 days. Asynchronous development was not observed in any of the treatments. Alterations, including follicular hypertrophy, were observed in thyroid gland histopathology in the control and each test concentration, but were not considered to be test substance-related. No clinical signs of sub-lethal toxicity were observed in the definitive test. The NOEC (No Observable Effect Concentration) for the test substance in a 21-day amphibian metamorphosis assay study using South African clawed frog, Xenopus laevis, was 186 μg/L based on time-weighted, mean measured methomyl concentrations and AMA endpoints. The LOEC (lowest observed effect concentration, for the same endpoint(s) was >186 μg/L.
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