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EC number: 240-815-0 | CAS number: 16752-77-5
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
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- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
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- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
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- Endpoint summary
- Stability
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- Environmental data
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- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data

Eye irritation
Administrative data
- Endpoint:
- eye irritation: in vivo
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 2 010
- Report date:
- 2010
Materials and methods
Test guidelineopen allclose all
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 405 (Acute Eye Irritation / Corrosion)
- Deviations:
- no
- Qualifier:
- according to guideline
- Guideline:
- EPA OPPTS 870.2400 (Acute Eye Irritation)
- Deviations:
- no
- Qualifier:
- according to guideline
- Guideline:
- other: Ministry of Agriculture, Forestry and Fisheries of Japan 12-Nouan-8147
- Deviations:
- no
- GLP compliance:
- yes
Test material
- Reference substance name:
- Methomyl
- EC Number:
- 240-815-0
- EC Name:
- Methomyl
- Cas Number:
- 16752-77-5
- Molecular formula:
- C5H10N2O2S
- IUPAC Name:
- (E)-[1-(methylsulfanyl)ethylidene]amino N-methylcarbamate
- Test material form:
- solid: crystalline
Constituent 1
- Specific details on test material used for the study:
- - Substance name: Methomyl Technical
- Substance ID: DPX-X1179
- Lot#: X1179-599
- Purity: 99.6%
Test animals / tissue source
- Species:
- rabbit
- Strain:
- other: New Zealand albino
- Details on test animals or tissues and environmental conditions:
- TEST ANIMALS
- Source: Robinson Services, Inc. Clemmons, North Carolina
- Age at study initiation: Young adult
- Weight at study initiation: 2930-3150 g
- Housing: One animal per cage
- Diet: ad libitum
- Water: ad libitum
- Acclimation period: 6-19 days
ENVIRONMENTAL CONDITIONS
- Temperature: 18-21°C
- Humidity: 26-59%
- Photoperiod: 12 hour light/dark cycle
Test system
- Vehicle:
- unchanged (no vehicle)
- Controls:
- yes, concurrent no treatment
- Amount / concentration applied:
- Initial: 0.08 g
Amount/concentration reduced to 0.009 g - Duration of treatment / exposure:
- The test substance was instilled into the conjunctival sac of the right eye of each rabbit by pulling the lower lid away from the eyeball. The upper and lower lids were then gently held together for about one second before releasing to minimize loss of the test substance.
- Observation period (in vivo):
- 1, 24, 48, and 72 hours
- Number of animals or in vitro replicates:
- 4 males and 1 female
- Details on study design:
- REMOVAL OF TEST SUBSTANCE
- Washing: Unwashed
SCORING SYSTEM: Draize method
TOOL USED TO ASSESS SCORE: High-intensity white light (Mag Lite)
Results and discussion
In vivo
Resultsopen allclose all
- Irritation parameter:
- cornea opacity score
- Basis:
- animal #1
- Time point:
- 24/48/72 h
- Score:
- 0
- Max. score:
- 4
- Remarks on result:
- no indication of irritation
- Irritation parameter:
- cornea opacity score
- Basis:
- animal #2
- Time point:
- 24/48/72 h
- Score:
- 0
- Max. score:
- 4
- Remarks on result:
- no indication of irritation
- Irritation parameter:
- cornea opacity score
- Basis:
- animal #3
- Time point:
- 24/48/72 h
- Score:
- 0
- Max. score:
- 4
- Remarks on result:
- no indication of irritation
- Irritation parameter:
- iris score
- Basis:
- animal #1
- Time point:
- 24/48/72 h
- Score:
- 0
- Max. score:
- 2
- Remarks on result:
- no indication of irritation
- Irritation parameter:
- iris score
- Basis:
- animal #2
- Time point:
- 24/48/72 h
- Score:
- 0
- Max. score:
- 2
- Remarks on result:
- no indication of irritation
- Irritation parameter:
- iris score
- Basis:
- animal #3
- Time point:
- 24/48/72 h
- Score:
- 0
- Max. score:
- 2
- Remarks on result:
- no indication of irritation
- Irritation parameter:
- conjunctivae score
- Basis:
- animal #1
- Time point:
- 24/48/72 h
- Score:
- 0.33
- Max. score:
- 3
- Reversibility:
- fully reversible within: 48 hours
- Irritation parameter:
- conjunctivae score
- Basis:
- animal #2
- Time point:
- 24/48/72 h
- Score:
- 0.67
- Max. score:
- 3
- Reversibility:
- fully reversible within: 72 hours
- Irritation parameter:
- conjunctivae score
- Basis:
- animal #3
- Time point:
- 24/48/72 h
- Score:
- 0.33
- Max. score:
- 3
- Reversibility:
- fully reversible within: 48 hours
- Irritation parameter:
- chemosis score
- Basis:
- animal #1
- Time point:
- 24/48/72 h
- Score:
- 0
- Max. score:
- 4
- Remarks on result:
- no indication of irritation
- Irritation parameter:
- chemosis score
- Basis:
- animal #2
- Time point:
- 24/48/72 h
- Score:
- 0
- Max. score:
- 4
- Remarks on result:
- no indication of irritation
- Irritation parameter:
- chemosis score
- Basis:
- animal #3
- Time point:
- 24/48/72 h
- Score:
- 0
- Max. score:
- 4
- Remarks on result:
- no indication of irritation
- Irritant / corrosive response data:
- There was no corneal opacity or iritis noted for any treated eye during the study. At the one hour scoring interval, the pupil of the treated eye of two of the three rabbits was constricted and the pupil of the treated eye of one of these rabbits did not respond to light. At 24 hours after instillation, the constriction/non-response to light of the pupils was not observed. Conjunctival redness (score of 1) was observed in the treated eye of three rabbits and discharge (score of 1) was observed in the treated eye of two rabbits. All animals were free of ocular irritation by 72 hours.
- Other effects:
- The rabbit dosed with one-tenth of a milliliter (0.08 grams) test substance was found dead within one hour of dosing. One of the rabbits dosed with 0.009 grams test substance was found dead within 24 hours of dosing. No clinical signs of systemic toxicity were observed in the decedents. White ocular discharge and clear oral discharge were observed in both decedents at gross necropsy. All surviving animals appeared active and healthy. No biologically relevant weight loss occurred in any of the surviving rabbits.
Applicant's summary and conclusion
- Interpretation of results:
- GHS criteria not met
- Conclusions:
- There was no corneal opacity or iritis noted. Conjunctival redness (score of 1) was observed in the treated eye of three rabbits and discharge (score of 1) was observed in the treated eye of two rabbits. All animals were free of ocular irritation by 72 hours.
- Executive summary:
A primary eye irritation test was conducted with rabbits to determine the potential for the test substance to produce irritation from a single instillation via the ocular route. The study was conducted according to OECD guideline 405 and U.S. EPA OPPTS 870.2400.
The study was conducted in a stepwise fashion. Initially, one-tenth of a milliliter (0.08 grams) of the ground test substance was instilled into the conjunctival sac of the right eye of one healthy rabbit by pulling the lower lid away from the eyeball. The upper and lower lids were then gently held together for about one second before releasing to minimize loss of the test substance. The left eye remained untreated and served as a control.
This rabbit was found dead within one hour of dosing. No clinical signs of systemic toxicity were observed. White ocular discharge and clear oral discharge were observed at gross necropsy.
Due to the mortality of this animal at the 0.1 mL dose level, subsequent testing was conducted using a lower dose amount.
A quantity of 0.009 g of the ground test substance was instilled into the conjunctival sac of the right eye of one healthy rabbit using the same procedures as above. Ocular irritation was evaluated by Draize method. Since irritation cleared by 48 hours and there was no significant irritation or adverse clinical signs observed in this animal, two additional animals were treated as described above. One of these animals died prior to the 24-hour scoring interval. No clinical signs of systemic toxicity were observed in the decedent. White ocular discharge and clear oral discharge were observed at gross necropsy. The rabbit that died was replaced and the replacement animal was dosed with 0.009 g of test substance.
There was no corneal opacity or iritis noted in the treated eye of the three surviving rabbits. At the one hour scoring interval, the pupil of the treated eye of two of the three rabbits was constricted and the pupil of the treated eye of one of these rabbits did not respond to light. At 24 hours after instillation, the constriction/non-response to light of the pupils was not observed. Conjunctival redness (score of 1) was observed in the treated eye of three rabbits and discharge (score of 1) was observed in the treated eye of two rabbits. All animals were free of ocular irritation by 72 hours.
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