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Environmental fate & pathways

Biodegradation in water and sediment: simulation tests

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The continuous activated sludge (CAS) test was performed according to ISO Guidelines, and in compliance with the OECD principles of Good Laboratory Practice. Tall oil, reaction products with N-(2-aminoethyl)piperazine were exposed to micro-organisms maintained by addition of domestic wastewater in the CAS test. Tall oil, reaction products with N-(2-aminoethyl)piperazine were spiked at a nominal influent concentration of 50 mg/L (36.5 mg/L carbon; calculated) for a period of 48 days and included a control fed with domestic wastewater only. The immediate high removal percentages can be attributed to adsorption and probably biodegradation. The mean removal percentage of tall oil, reaction products with N-(2-aminoethyl)piperazine calculated over 15 measurements obtained from day 34 to 48 of the test was 89 +/-1% (95% confidence interval). These organic carbon removal percentages in excess of the pass level of 80% indicate that the test substance is completely removed. An accurate assessment of the removal of tall oil, reaction products with N-(2- aminoethyl)piperazine was established with specific analyses. The method (LCMS/ MS) for the determination of tall oil, reaction products with N-(2- aminoethyl)piperazine was satisfactory with regard to the linearity, repeatability of the injections, limit of quantification (LOQ), recovery and specificity. The mean removal percentage of tall oil, reaction products with N-(2-aminoethyl)piperazine in the test unit was assessed with the specific analysis from day 44 to 48 was >99.999% using octadecenyl amide of N-(2-aminoethyl)piperazine as representative component. These analyses demonstrate that the removal of tall oil, reaction products with N-(2-aminoethyl)piperazine is complete. Tall oil, reaction products with N-(2-aminoethyl)piperazine concentrations in the sludge of the reactor sampled on days 47 and 48 were 0.88 and 0.80 mg/L. The mean removal percentage of tall oil, reaction products with N-(2-aminoethyl)piperazine from the influent through adsorption onto sludge assessed in two samples was therefore 0.04 -0.044%. This percentage demonstrates that tall oil, reaction products with N-(2-aminoethyl)piperazine are almost completely removed by biodegradation. The CAS test demonstrates that tall oil, reaction products with N- (2-aminoethyl)piperazine almost completely removed from the wastewater by biodegradation in conventional biological wastewater treatment plants.