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Ecotoxicological information

Short-term toxicity to fish

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Description of key information

Based on OECD guideline studies, amides, C16-18 (even-numbered) and C18-unsatd., N,N-bis(hydroxyethyl) have shown acute 96 hour LC50 values in freshwater fish ranging from 1.2 to 4.9 mg/L.

Key value for chemical safety assessment

Fresh water fish

Fresh water fish
Effect concentration:
1.2 mg/L

Additional information

The acute 96 hour LC50values of freshwater fish range from 1.2 to 4.9 mg/L.

A study was conducted to assess the acute toxicity of the amides, C16 -18 (even-numbered) and C18 -unsatd., N,N-bis(hydroxyethyl) to rainbow trout according to the OECD guideline 203. Ten fish per vessel were exposed to the test substance at concentrations of 0.1, 0.3, 1.0, 3.0, 10.0 and 30.0 mg/L for 96 hours under static conditions. Mortality and other sub-lethal effects such as swimming at the bottom and surface, loss of equilibrium and moribundity were recorded at 3, 6, 24, 48, 72 and 96 hours. Analysis of the mortality data, by the moving average method of Thompson (1947) revealed the LC50values at 24, 48, 72 and 96 hours as 2.5, 1.7, 1.3 and 1.2 mg/L respectively. The NOEC and LC100of the test substance were determined at 0.3 and 3.0 mg/L, respectively. Further, the sub-lethal effects were observed at concentrations >1.0 mg/L. Under the test conditions, the test substance was considered to be toxic to rainbow trout (Wetton PM, 1997).

Another study was conducted to assess the acute toxicity of the amides, C16-18 (even-numbered) and C18-unsatd., N,N-bis(hydroxyethyl) in Zebra fish according to ISO guideline7346/2 (which conforms to the OECD-guideline 203). Ten fish per tank were exposed to 2.8 to16 mg/L concentrations of the test substance (87% purity) in freshly prepared standard dilution water for a period of 96 hours under semi-static conditions. Mortality rates and abnormal behaviour were recorded at 0, 6, 24, 48, 72 and 96 hours.Under the test conditions, the test substance was found to be toxic to Zebra fish with an LC50value of 5.6 mg/L (based on nominal concentration) i.e., equivalent to 4.9 mg active matter/L (Gode, 2001).