Allyl 2,3-epoxypropyl ether

Genetic toxicity: in vivo

Administrative data

Endpoint:

in vivo mammalian somatic cell study: cytogenicity / erythrocyte micronucleus

Remarks:

Type of genotoxicity: chromosome aberration

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

other: Basic data given: scientifically acceptable study summary including result tables; the test protocol can be viewed online.

Data source

Reference

Reference Type:

publication

Title:

Unnamed

Year:

1989

Materials and methods

Principles of method if other than guideline:

According to the NTP standard protocols

GLP compliance:

no

Type of assay:

micronucleus assay

Test material

Test material information

Constituent 1

Reference substance name:

Allyl 2,3-epoxypropyl ether

EC Number:

203-442-4

EC Name:

Allyl 2,3-epoxypropyl ether

Cas Number:

106-92-3

Molecular formula:

C6H10O2

IUPAC Name:

2-[(prop-2-en-1-yloxy)methyl]oxirane

Details on test material:

- Name of test material (as cited in study report): Allyl glycidyl ether

Test animals

Species:

mouse

Strain:

not specified

Sex:

male

Administration / exposure

Route of administration:

intraperitoneal

Vehicle:

- Vehicle(s)/solvent(s) used: physiol. saline

Frequency of treatment:

3, administered at 24 hr intervals

Doses / concentrations

Remarks:

Doses / Concentrations:
50, 100 and 200 mg/kg bw
Basis:

No. of animals per sex per dose:

3-5 (3 males were used in trial 1 at dose level of 200 mg/kg bw; in the 2nd trial, 5, 4 and 5 mice were used at dose levels of 50, 100 and 200 mg/kg bw, respectively)

Control animals:

yes, concurrent vehicle

Positive control(s):

mitomycin C
- Doses / concentrations: 0.2 mg/kg bw

Examinations

Tissues and cell types examined:

bone marrow

Details of tissue and slide preparation:

TREATMENT AND SAMPLING TIMES: harvesting of the bone marrow done at 24 hours after the last dosing.

DETAILS OF SLIDE PREPARATION: The animals are euthanized by CO2 inhalation and the femurs are removed. The bone marrow is flushed from the femurs and spread onto slides. The slides are air-dried, fixed, and stained with a fluorescent DNA-specific stain that easily illuminates any micronuclei that may be present. Typically, 2000 polychromatic erythrocytes (PCEs, reticulocytes; immature erythrocytes) are scored per animal for frequency of micronucleated cells in each of 3-5 animals per dose group.

Results and discussion

Test results

Sex:

male

Genotoxicity:

positive

Toxicity:

not specified

Vehicle controls validity:

valid

Positive controls validity:

valid

Any other information on results incl. tables

     Trial 1:

	Dose (mg/kg)	Number of Animals Scored	Mean MN-PCE/1000 PCE ± SEM	Pairwise P
Phosphate Buffered Saline	0	3	1.67 ± 1.20
Allyl glycidyl ether	200	3	14.67 ± 2.03	< 0.0001
Mitomycin-C	0.2	2	10.50 ± 3.50	*

    * Insufficient animals scored to calculate p-value.

   

    Trial 2:

	Dose (mg/kg)	Number of Animals Scored	Mean MN-PCE/1000 PCE ± SEM	Pairwise P
Phosphate Buffered Saline	0	3	1.67 ± 1.20
Allyl glycidyl ether	50	5	4.40 ± 0.43	0.001
	100	4	5.88 ± 0.90	< 0.0001
	200	5	20.00 ± 2.01	< 0.0001
Mitomycin-C	0.2	5	6.50 ± 0.42	< 0.0001