Difluoroacetic acid

Endpoint summary

Description of key information

Short-term toxicity to fish

The lethal concentration (LC50) value of difluoroacetic acid in fish (Pimephales promelas) in a 96 hr study on the basis of mortality effect was estimated to be 165.50 mg/L. Based on this prediction it can be inferred that the target chemical is nontoxic to aquatic environment. The BCF value of dodecan-1-ol, ethoxylated reported was not >= 500 as well as the substance was found to be rapidly degradable

 

No. of studies reviewed for short – term toxicity to fish from reliable sources having Klimisch rating 2 and 4.

 

The summary of the results are presented below

  

Sr. No.	Endpoint	Effect values	Basis for effects	Duration	Species	Sources
1	LC50	165.50 mg/L	Mortality	96 hrs	Pimephales promelas	Predicted data from QSAR
2	LC50	121.88 mg/L	Mortality	96 hrs	Lepomis macrochirus	Predicted data from QSAR
3	LC50	52695.99 mg/L	Mortality	96 hrs	Fish	Predicted data from ECOSAR
4	LC50	85971.10 mg/L	Mortality	96 hrs	Fish	Predicted data from ECOSAR

  

By applying weight of evidence approach to the test substance difluoroacetic acid, the endpoint value was found to vary between LC50 = 121.88 mg/L to 165.50 mg/L in a 96 hrs. also found 52695.99 mg/L & 52695.99 mg/L at 96 hrs. study on the basis of mortality effect. The BCF value of difluoroacetic acid reported was not >= 500 as well as the substance was found to be rapidly degradable. Since the additional criteria mentioned in the CLP regulation related to BCF, biodegradability and Log Kow is not satisfied thus the substance has not been considered for aquatic toxicity classification.

 

Long term toxicity to fish:

Based on the prediction done using ECOSAR version 1.1, the long term toxicity on fish was predicted for test substance . On the basis of effects observed in a static freshwater system, the NOEC value for the test substance is estimated to be4767.749mg/l for fish for 28 days of exposure duration.Since, the test substance is readily biodegradable , it can be concluded that the test chemicalcan be considered as non-toxic to fish at environmentally relevant concentrations and can be considered not-classified as per the CLP classification criteria. 

Short-term toxicity to aquatic invertebrates

 

 

No. of studies reviewed for short – term toxicity to fish from reliable sources having Klimisch rating 2 and 4.

The summary of the results are presented below

 

Sr. No.	Endpoint	Effect values	Basis for effects	Duration	Species	Sources
1	LC50	235.79 mg/L	Mortality	48 hrs	Daphnia magna	Predicted data from QSAR
2	LC50	21767.93 mg/L	Mortality	48 hrs	Daphnia magna	Predicted data from ECOSAR

 

By applying weight of evidence approach to the test substance difluoroacetic acid, the endpoint value was found to LC50 = 235.79 mg/L & 21767.93 mg/L in a 48 hrs. to 48 hrs. study on the basis of mortality effect. The BCF value of difluoroacetic acid reported was not >= 500 as well as the substance was found to be rapidly degradable

Long term toxicity to aquatic invertebrate:

Based on the prediction done using ECOSAR version 1.1, the long term toxicity on aquatic invertebrate was predicted for test substance . On the basis of effects observed in a static freshwater system, the NOEC value for the test substance is estimated to be1628.246mg/l for aquatic invertebrate for 21 days of exposure duration.Since, the test substance is readily biodegradable , it can be concluded that the test chemical can be considered as non-toxic to aquatic invertebrate at environmentally relevant concentrations and can be considered not-classified as per the CLP classification criteria.

Toxicity to aquatic algae and cyanobacteria

No. of studies reviewed for short – term toxicity to aquatic algae and cyanobacteria from reliable sources having Klimisch rating 2 and 4.

The summary of the results are presented below

  

Sr. No.	Endpoint	Effect values	Basis for effects	Duration	Species	Sources
1	NOEC	106.73 mg/L	Growth	72 hrs	Pseudokirchneriella subcapitata	Predicted data from QSAR
2	LC50	4141.50 mg/L	---	96 hrs	Green Algae	Predicted data from ECOSAR

 

By applying weight of evidence approach to the test substance difluoroacetic acid, the endpoint value was found to NOEC = 106.73 mg/L at 72 Hrs & LC50 at 4141.50 mg/L in a 96 hrs. at 72 hrs. study on the basis of growth effect. The BCF value of difluoroacetic acid reported was not >= 500 as well as the substance was found to be rapidly degradable

 

Toxicity to microorganisms

The effective concentration (EC50) value of difluoroacetic acid in microorganism (Tetrahymena pyriformis) in a 48 hr study on the basis of growth effect was estimated to be 101.2192 mg/L.

Plant Toxicity:

The Nominal concentration of Dichloro acetic acid were 0, 10, 30 & 100 mg/L on Myriophyllum spicatum, M. sibiricum, and Lemna gibba. On which the NOEC & LOEC value was found to be on M. spicatum at Day 14 NOEC was 30 & LOEC 100mg/L., for M. sibiricum day 14 the NOEC 10 & LOEC on 30 mg/L and for L. gibba the NOEC on 14 days at 30 mg/L & LOEC was found to be at 100 mg/Lon Frond number.

Additional information

Short-term toxicity to fish

The lethal concentration (LC50) value of difluoroacetic acid in fish (Pimephales promelas) in a 96 hr study on the basis of mortality effect was estimated to be 165.50 mg/L. Based on this prediction it can be inferred that the target chemical is nontoxic to aquatic environment. The BCF value of dodecan-1-ol, ethoxylated reported was not >= 500 as well as the substance was found to be rapidly degradable

 

No. of studies reviewed for short – term toxicity to fish from reliable sources having Klimisch rating 2 and 4.

 

The summary of the results are presented below

  

Sr. No.	Endpoint	Effect values	Basis for effects	Duration	Species	Sources
1	LC50	165.50 mg/L	Mortality	96 hrs	Pimephales promelas	Predicted data from QSAR
2	LC50	121.88 mg/L	Mortality	96 hrs	Lepomis macrochirus	Predicted data from QSAR
3	LC50	52695.99 mg/L	Mortality	96 hrs	Fish	Predicted data from ECOSAR
4	LC50	85971.10 mg/L	Mortality	96 hrs	Fish	Predicted data from ECOSAR

  

By applying weight of evidence approach to the test substance difluoroacetic acid, the endpoint value was found to vary between LC50 = 121.88 mg/L to 165.50 mg/L in a 96 hrs. also found 52695.99 mg/L & 52695.99 mg/L at 96 hrs. study on the basis of mortality effect. The BCF value of difluoroacetic acid reported was not >= 500 as well as the substance was found to be rapidly degradable. Since the additional criteria mentioned in the CLP regulation related to BCF, biodegradability and Log Kow is not satisfied thus the substance has not been considered for aquatic toxicity classification.

 

Long term toxicity to fish:

Based on the prediction done using ECOSAR version 1.1, the long term toxicity on fish was predicted for test substance . On the basis of effects observed in a static freshwater system, the NOEC value for the test substance is estimated to be4767.749mg/l for fish for 28 days of exposure duration.Since, the test substance is readily biodegradable , it can be concluded that the test chemicalcan be considered as non-toxic to fish at environmentally relevant concentrations and can be considered not-classified as per the CLP classification criteria. 

Short-term toxicity to aquatic invertebrates

 

 

No. of studies reviewed for short – term toxicity to fish from reliable sources having Klimisch rating 2 and 4.

The summary of the results are presented below

 

Sr. No.	Endpoint	Effect values	Basis for effects	Duration	Species	Sources
1	LC50	235.79 mg/L	Mortality	48 hrs	Daphnia magna	Predicted data from QSAR
2	LC50	21767.93 mg/L	Mortality	48 hrs	Daphnia magna	Predicted data from ECOSAR

 

By applying weight of evidence approach to the test substance difluoroacetic acid, the endpoint value was found to LC50 = 235.79 mg/L & 21767.93 mg/L in a 48 hrs. to 48 hrs. study on the basis of mortality effect. The BCF value of difluoroacetic acid reported was not >= 500 as well as the substance was found to be rapidly degradable

Long term toxicity to aquatic invertebrate:

Based on the prediction done using ECOSAR version 1.1, the long term toxicity on aquatic invertebrate was predicted for test substance . On the basis of effects observed in a static freshwater system, the NOEC value for the test substance is estimated to be1628.246mg/l for aquatic invertebrate for 21 days of exposure duration.Since, the test substance is readily biodegradable , it can be concluded that the test chemical can be considered as non-toxic to aquatic invertebrate at environmentally relevant concentrations and can be considered not-classified as per the CLP classification criteria.

Toxicity to aquatic algae and cyanobacteria

No. of studies reviewed for short – term toxicity to aquatic algae and cyanobacteria from reliable sources having Klimisch rating 2 and 4.

The summary of the results are presented below

   

Sr. No.	Endpoint	Effect values	Basis for effects	Duration	Species	Sources
1	NOEC	106.73 mg/L	Growth	72 hrs	Pseudokirchneriella subcapitata	Predicted data from QSAR
2	LC50	4141.50 mg/L	---	96 hrs	Green Algae	Predicted data from ECOSAR

 

By applying weight of evidence approach to the test substance difluoroacetic acid, the endpoint value was found to NOEC = 106.73 mg/L at 72 Hrs & LC50 at 4141.50 mg/L in a 96 hrs. at 72 hrs. study on the basis of growth effect. The BCF value of difluoroacetic acid reported was not >= 500 as well as the substance was found to be rapidly degradable

 

Toxicity to microorganisms

The effective concentration (EC50) value of difluoroacetic acid in microorganism (Tetrahymena pyriformis) in a 48 hr study on the basis of growth effect was estimated to be 101.2192 mg/L.

Plant Toxicity:

The Nominal concentration of Dichloro acetic acid were 0, 10, 30 & 100 mg/L on Myriophyllum spicatum, M. sibiricum, and Lemna gibba. On which the NOEC & LOEC value was found to be on M. spicatum at Day 14 NOEC was 30 & LOEC 100mg/L., for M. sibiricum day 14 the NOEC 10 & LOEC on 30 mg/L and for L. gibba the NOEC on 14 days at 30 mg/L & LOEC was found to be at 100 mg/Lon Frond number.