1-methylpyrrolidine

Acute Toxicity: inhalation

Administrative data

Endpoint:

acute toxicity: inhalation

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

other: scientifically acceptable study report

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1980

Report date:

1980

Materials and methods

Test guideline

Qualifier:

equivalent or similar to guideline

Guideline:

OECD Guideline 403 (Acute Inhalation Toxicity)

Deviations:

yes

Remarks:

exposure time only 1 hour

Principles of method if other than guideline:

Dynamic inhalation method; whole-body - head/nose exposure with analytical and nominal concentration determination.

GLP compliance:

no

Test type:

standard acute method

Limit test:

no

Test material

Test material information

Constituent 1

Reference substance name:

1-methylpyrrolidine

EC Number:

204-438-5

EC Name:

1-methylpyrrolidine

Cas Number:

120-94-5

Molecular formula:

C5H11N

IUPAC Name:

1-methylpyrrolidine

Test material form:

other: liquid

Test animals

Species:

rat

Strain:

Sprague-Dawley

Sex:

male/female

Details on test animals or test system and environmental conditions:

Mean body weight: 200 - 300 g
The animals were held in air-conditioned rooms with a room temperature of 22 +/- 2°C and a relative humidity of 55 +/- 10%.
Air changing: 10- to 15-fold
Day/night rhythm: 12 h dark, 12 h light
Type of cage: Makrolon type 3 (23 x 39cm2)
Animals per cage: 2
Animal identification: via ear marks
The animals were offered a standard animal laboratory diet as well as drinking water ad libitum.
TEST ANIMALS
- Weight at study initiation: 200-300 g
- Housing: 2 animals per cage, Makrolon type 3 (23 x 39cm2)
- Diet: standard animal laboratory diet, ad libitum
- Water: ad libitum

ENVIRONMENTAL CONDITIONS
- Temperature (°C): of 22 +/- 2°C
- Humidity (%): 55 +/- 10%.
- Air changes (per hr): 10-15
- Photoperiod (hrs dark / hrs light): 12 h dark, 12 h light

Administration / exposure

Route of administration:

inhalation: aerosol

Type of inhalation exposure:

whole body

Vehicle:

air

Details on inhalation exposure:

GENERATION OF TEST ATMOSPHERE / CHAMBER DESCRIPTION
- Exposure apparatus: Makrolon animal tubes
- Exposure chamber volume: 28.5 L
- Source and rate of air: continuous airation, 600 L/h
- System of generating particulates/aerosols: diffuser nozzle

TEST ATMOSPHERE
- Samples taken from breathing zone: yes

Analytical verification of test atmosphere concentrations:

yes

Duration of exposure:

1 h

Concentrations:

4.5 and 10.1 mg/L (nominal)

No. of animals per sex per dose:

10

Details on study design:

- Duration of observation period following administration: 14 days
- Necropsy of survivors performed: yes
- Other examinations performed: clinical signs

Results and discussion

Effect levels

Sex:

male/female

Dose descriptor:

LC50

Effect level:

> 4.5 - < 10.1 mg/L air

Based on:

test mat.

Exp. duration:

1 h

Remarks on result:

other: by applying Habers' law (c x t = k) for time extrapolation (1 h to 4 h exposure): LC50 > 1.1 < 2.5 mg/L air

Mortality:

4.5 mg/L: no mortality
10.1 mg/L: all animals died

Clinical signs:

other: During exposure: In the lowest concentration male and female rats showed a slight reduced spontaneous activity as well as a slight reduced rection of outer stimuli; furthermore slight gasping, slight constricted palpebral fissures and slight increased sa

Body weight:

not examined

Gross pathology:

Animals that died (high concentration): 10 of 20 rats showed the following changes of the lung: alveolar emphysema, petechiae and focal atelectasis.
Sacrificed animals: 1 of 20 rats showed isolated petechia (low concentration).