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EC number: 204-438-5 | CAS number: 120-94-5
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data

Genetic toxicity: in vitro
Administrative data
- Endpoint:
- in vitro gene mutation study in bacteria
- Remarks:
- Type of genotoxicity: gene mutation
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- other: GLP- and guideline study
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 1 998
- Report date:
- 1998
Materials and methods
Test guidelineopen allclose all
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 471 (Bacterial Reverse Mutation Assay)
- Version / remarks:
- 1983
- Deviations:
- no
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 472 (Genetic Toxicology: Escherichia coli, Reverse Mutation Assay)
- Version / remarks:
- 1996
- Deviations:
- no
- Qualifier:
- according to guideline
- Guideline:
- EU Method B.13/14 (Mutagenicity - Reverse Mutation Test Using Bacteria)
- Version / remarks:
- 1992
- Deviations:
- no
- GLP compliance:
- yes (incl. QA statement)
- Remarks:
- testing lab.
- Type of assay:
- bacterial reverse mutation assay
Test material
- Reference substance name:
- 1-methylpyrrolidine
- EC Number:
- 204-438-5
- EC Name:
- 1-methylpyrrolidine
- Cas Number:
- 120-94-5
- Molecular formula:
- C5H11N
- IUPAC Name:
- 1-methylpyrrolidine
Constituent 1
Method
Species / strainopen allclose all
- Species / strain / cell type:
- S. typhimurium TA 1535, TA 1537, TA 98 and TA 100
- Species / strain / cell type:
- E. coli WP2 uvr A
- Metabolic activation:
- with and without
- Metabolic activation system:
- Aroclor-induced rat liver S-9 mix
- Test concentrations with justification for top dose:
- 20, 100, 500, 2500, 5000 µg/plate (standard plate test)
125, 250, 500, 1000, 1500 µg/plate (preincubation test) - Vehicle / solvent:
- water
Controls
- Untreated negative controls:
- yes
- Negative solvent / vehicle controls:
- yes
- True negative controls:
- no
- Positive controls:
- yes
- Positive control substance:
- other: with S9-mix: 2-aminoanthracene (all strains); without S9-mix: 2-aminoanthracene (all strains), N-methyl-N'-nitro-N-nitrosoguanidine (TA1535, 100), 4-nitro-o-phenylenediamine (TA98), 9-aminoacridine (TA1537) and N-ethyl-N'-nitro-N-nitrosoguanidine (E.coli)
- Details on test system and experimental conditions:
- METHOD OF APPLICATION: in agar (plate incorporation); preincubation
DURATION
- Preincubation period: 20 min
- Expression time (cells in growth medium): 48-72 h
NUMBER OF REPLICATIONS: 3
DETERMINATION OF CYTOTOXICITY
- Method: relative total growth - Evaluation criteria:
- The test chemical is considered positive in this assay if the following criteria are met:
A dose-related and reproducable increase in the number of revertant colonies, i.e. about doubling of the spontaneous mutation rate in at least one tester strain either without S-9 mix or after adding a metabolizing system.
A test substance is generally considered nonmutagenic in this test if:
The number of revertants for all tester strains were within the historical negative control range under all experimental conditions in two experiments carried out independently of each other. - Statistics:
- The arithmetic mean of the counted colonies per concentration was calculated.
Results and discussion
Test resultsopen allclose all
- Species / strain:
- S. typhimurium TA 1535, TA 1537, TA 98 and TA 100
- Metabolic activation:
- with and without
- Genotoxicity:
- negative
- Cytotoxicity / choice of top concentrations:
- cytotoxicity
- Remarks:
- A bacteriotoxic effect was observed in the preincubation test depending an the strain and test conditions at about > 1000 µg/plate.
- Vehicle controls validity:
- valid
- Untreated negative controls validity:
- valid
- Positive controls validity:
- valid
- Species / strain:
- E. coli WP2 uvr A
- Metabolic activation:
- with and without
- Genotoxicity:
- negative
- Cytotoxicity / choice of top concentrations:
- other: A bacteriotoxic effect was observed in the preincubation test depending an the strain and test conditions at about > 1000 ug/plate.
- Vehicle controls validity:
- valid
- Untreated negative controls validity:
- valid
- Positive controls validity:
- valid
- Additional information on results:
- An increase in the number of his+ or trp+ revertants was not observed in the standard plate test or in the preincubation test either without S-9 mix or after the addition of a metabolizing system.
- Remarks on result:
- other: all strains/cell types tested
- Remarks:
- Migrated from field 'Test system'.
Any other information on results incl. tables
Table 1: Number of revertants per plate (mean of three plates), test 1
strain TA98 |
strain TA100 |
strain TA1535 |
strain TA1537 |
|||||
conc. [µg/mL] |
-MA |
+MA |
-MA |
+MA |
-MA |
+MA |
-MA |
+MA |
0 (water) |
27 |
46 |
159 |
158 |
20 |
20 |
12 |
|
20 |
27 |
46 |
157 |
158 |
20 |
22 |
10 |
|
100 |
26 |
37 |
157 |
159 |
19 |
20 |
11 |
|
500 |
22 |
29 |
127 |
157 |
20 |
16 |
10 |
|
2500 |
|
8 |
|
83 |
|
8 |
|
|
5000 |
|
|
|
|
|
|
|
|
4-nitro-o-phenyleridiamine 10 µg |
1266 |
|
|
|
|
|
|
|
2-Aminoanthracene 2.5 µg |
|
1215 |
|
1536 |
|
135 |
|
147 |
N-methyl-N' -nitro-N-nitrosoguanidine 5 µg |
|
|
2239 |
|
1424 |
|
|
|
9-aminoacridine 100 µg |
|
|
|
|
|
|
472 |
|
Table 2: Number of revertants per plate (mean of three plates), test 2
strain TA98 |
strain TA100 |
strain TA1535 |
strain TA1537 |
E. coli WP2 uvrA |
||||||
conc. [µg/mL] |
-MA |
+MA |
-MA |
+MA |
-MA |
+MA |
-MA |
+MA |
-MA |
+MA |
0 (water) |
30 |
48 |
137 |
151 |
13 |
12 |
10 |
11 |
33 |
38 |
125 |
28 |
39 |
141 |
154 |
13 |
15 |
13 |
11 |
32 |
39 |
250 |
35 |
46 |
136 |
152 |
16 |
12 |
9 |
10 |
33 |
35 |
500 |
35 |
46 |
158 |
139 |
12 |
15 |
9 |
12 |
35 |
41 |
1000 |
28 |
46 |
104 |
119 |
10 |
15 |
6 |
9 |
22 |
37 |
1500 |
34 |
38 |
108 |
135 |
- |
12 |
1 |
12 |
17 |
27 |
4-nitro-o-phenyleridiamine 10 µg |
1113 |
|
|
|
|
|
|
|
|
|
2-Aminoanthracene 2.5 µg* |
|
786 |
|
662 |
|
88 |
|
129 |
|
112 |
N-methyl-N' -nitro-N-nitrosoguanidine 5 µg |
|
|
1297 |
|
1083 |
|
|
|
|
|
9-aminoacridine 100 µg |
|
|
|
|
|
|
346 |
|
|
|
N-ethylN-nitro-N-nitrosoguanidine 10 µg |
|
|
|
|
|
|
|
|
772 |
|
*except for E.coli: 60 µg
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