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Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.

The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.

Diss Factsheets

Administrative data

biodegradation in soil: simulation testing
Type of information:
experimental study
Adequacy of study:
supporting study
3 (not reliable)
Rationale for reliability incl. deficiencies:
other: Despite no guideline being available in 1966, methods are reproducible and straightforward, and results are reasonable.

Data source

Reference Type:
Report date:

Materials and methods

Principles of method if other than guideline:
Test solution contained substituted benzenes as the sole carbon source. Inoculum was Niagara silt loam. Absorbancy of the centrifuged supernatant aliquot was monitored up to 64 days to monitor cleavage of benzene ring (cleavage results in loss of UV absorbancy).
GLP compliance:
Test type:

Test material

Constituent 1
Chemical structure
Reference substance name:
EC Number:
EC Name:
Cas Number:
Molecular formula:
Details on test material:
No details provided in the publication.

Study design

Oxygen conditions:
Soil classification:
other: Niagara silt loam

Results and discussion

Half-life / dissipation time of parent compound
Remarks on result:
other: Neither of the phenylenediamine isomers (ortho and meta) exhibited ring cleavage within 64 days.

Any other information on results incl. tables

Neither of the phenylenediamine isomers (ortho and meta) exhibited ring cleavage within 64 days.

Most of the substituted anilines were not suitable substrates under the test conditions. The resistance of the anilines was rather surprising because amino compounds are universal cellular constituents, by contrast with nitro, chloro, and sulfonate compounds. Seven of the 15 anisoles were also largely inert when exposed to the mixed soil population, but six of the seven contained either a nitro or an amino substituent.

Applicant's summary and conclusion

Neither of the phenylenediamine isomers (ortho and meta) exhibited ring cleavage within 64 days.
Executive summary:

The rate of degradation of mono- and disubstituted benzenes (approx. 80 substances) by soil microorganisms was determined by a spectrophotometric technique. Chloro, sulfonate, and nitro groups retarded the rate of biodegradation whereas carboxyl and phenolic hydroxyl groups favored decomposition of the substituted benzenes. The meta isomer was commonly the most resistant to attack by soil microorganisms, but the ortho isomer was the most resistant for certain classes of compounds.


The loss of UV absorbancy was not observed over the 64 day period for ortho and meta phenylenediamine indicating slow decomposition under test conditions.