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EC number: 202-430-6 | CAS number: 95-54-5
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data

Dermal absorption
Administrative data
- Endpoint:
- dermal absorption in vitro / ex vivo
- Type of information:
- experimental study
- Adequacy of study:
- supporting study
- Reliability:
- 3 (not reliable)
- Rationale for reliability incl. deficiencies:
- significant methodological deficiencies
- Remarks:
- Insufficient information to adequately evaluate data. Did not include recovery data or exposure duration; Focus was on correlating percutaneous absorption to partition coefficients
Data source
Reference
- Reference Type:
- publication
- Title:
- Percutaneous absorption of hair dyes: correlation with partition coefficients
- Author:
- Bronaugh RL, Congdon ER
- Year:
- 1 984
- Bibliographic source:
- J Invest Dermatol, 83:124-127
Materials and methods
Test guideline
- Qualifier:
- equivalent or similar to guideline
- Guideline:
- OECD Guideline 428 (Skin Absorption: In Vitro Method)
- Deviations:
- yes
- Remarks:
- No information on exposure time, preparation of test material or recovery data
- GLP compliance:
- not specified
Test material
- Reference substance name:
- o-phenylenediamine
- EC Number:
- 202-430-6
- EC Name:
- o-phenylenediamine
- Cas Number:
- 95-54-5
- Molecular formula:
- C6H8N2
- IUPAC Name:
- benzene-1,2-diamine
- Details on test material:
- - Name of test material (as cited in study report): o-phenylenediamine (PDA)
- Source: Aldrich Chemical Co.
- Analytical purity: 98%.
- Radiochemical purity (if radiolabelling): 98%. (for binding studies only)
- Specific activity (if radiolabelling): 4.75 mCi/mmol. [14C]o-phenylenediamine was from Pathfinder Laboratories
Constituent 1
- Radiolabelling:
- yes
- Remarks:
- [14C]o-phenylenediamine
Test animals
- Species:
- human
Administration / exposure
- Type of coverage:
- open
- Vehicle:
- other: Aqueous
- Duration of exposure:
- No data
- Doses:
- 0.5 mL over 1.13 cm2 of exposed skin
- No. of animals per group:
- No data
Results and discussion
Any other information on results incl. tables
The percutaneous absorption of p-PDA, 2-nitro-p-PDA, 2 -amino-4 -nitrophenol, 4 -chloro-m-phenylenediamine, and 4 -amino-2 -nitrophenol were also tested in this study. The octanol/water partition coefficients were generally in the same rank order as the permeability constants. Preliminary stratum corneum/water partition studies resulted in values that were in the reverse order of skin permeation. When binding of the compounds to components of the membrane were saturated, the partition values more closely duplicated the rank order of permeability of dyes.
Percutaneous Absorption of Hair dyes
Compound |
Octanol/water partition coefficient |
Stratum corneum/water partition coefficient |
Permeability constant cm/h |
o-phenylenediamine |
1.4 |
6.9 |
4.5 x10-4 |
Dependence of Km on Concentration of o-phenylenediamine
Initial Concentration |
Equilibrium Concentration |
Km (bound/free) |
||
(DPM/10uL) |
(M x 105) |
Bound (M x 104) |
Free (M x 105) |
|
80462 |
77.2 |
46.9 |
68.4 |
6.86 |
36685 |
35.2 |
27.5 |
29.5 |
9.32 |
11393 |
10.9 |
14.6 |
8.01 |
18.2 |
3723 |
3.57 |
6.30 |
2.33 |
27.0 |
1372 |
1.32 |
2.69 |
0.79 |
34.2 |
474 |
0.45 |
1.09 |
0.24 |
45.0 |
Applicant's summary and conclusion
- Conclusions:
- Prediction of the percutaneous absorption of a substance based on its partition coefficient may be confounded if the compound is capable of binding to the skin.
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