Registration Dossier
Registration Dossier
Use of this information is subject to copyright laws and may require the permission of the owner of the information, as described in the ECHA Legal Notice.
EC number: 202-430-6 | CAS number: 95-54-5
Toxicity to terrestrial plants
Administrative data
- Endpoint:
- toxicity to terrestrial plants: short-term
- Type of information:
- experimental study
- Adequacy of study:
- supporting study
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- study well documented, meets generally accepted scientific principles, acceptable for assessment
Data source
Reference
- Reference Type:
- publication
- Title:
- Induction of Somatic Mutations in Tradescantia clone 4430 by Three Phenylenediamine Isomers and the Antimutagenic Mechanisms of Diethyldithiocarbamate and Ammonium Meta-Vanadate
- Author:
- Gichner T, Cabrera Lopez G, Wagner ED, and Plewa MJ
- Year:
- 1 994
- Bibliographic source:
- Mutation Research, 306: 165-172
Materials and methods
Test guideline
- Qualifier:
- no guideline followed
- Principles of method if other than guideline:
- Mutagenicity assay in plants was reported.
- GLP compliance:
- no
Test material
- Reference substance name:
- o-phenylenediamine
- EC Number:
- 202-430-6
- EC Name:
- o-phenylenediamine
- Cas Number:
- 95-54-5
- Molecular formula:
- C6H8N2
- IUPAC Name:
- benzene-1,2-diamine
- Details on test material:
- - Name of test material (as cited in study report): o-phenylenediamine
- Physical state: Solid
Constituent 1
Sampling and analysis
- Analytical monitoring:
- no
Test substrate
- Vehicle:
- yes
Test organisms
- Species:
- other: Tradescantia clone 4430
- Details on test organisms:
- a hybrid of T. subacaulis X T. hirsutiflora cultivated in a greenhouse at the Institute of Experimental Botany, Prague
Study design
- Test type:
- other: Mutagenicity assay
- Study type:
- laboratory study
- Total exposure duration:
- 15 d
Test conditions
- Test temperature:
- 18-22°C
- Nominal and measured concentrations:
- 25, 50, 75, 100, 150, and 200 mM o-phenylenediamine dissolved in 0.2 M citrate-phosphate buffer, plus control (citrate-phosphate buffer only).
Results and discussion
Any other information on results incl. tables
A concentration dependent increase in pink stamen hair mutations was seen in the concentration range of 50-150 mM o-phenylenediamine.
o-Phenylenediamine was found to be toxic at concentrations above 150 mM, and repressed flowering.
At 150 mM, o-phenylenediamine induced approximately a 28-fold increase in the mutant frequency over the negative control.
Applicant's summary and conclusion
- Conclusions:
- Under the conditions employed in this study, o-phenylenediamine is mutagenic to Tradescantia clone 4430 relative to the negative control. The mutagenic potency of o-phenylenediamine in Tradescantia was 5.60 stamen hair mutants/µmole.
- Executive summary:
A mutagenicity assay in plants was conducted. Under the conditions employed in this study, o-phenylenediamine is mutagenic to Tradescantia clone 4430 relative to the negative control. The mutagenic potency of o-phenylenediamine in Tradescantia was 5.60 stamen hair mutants/µmole.
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.
