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Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.

The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.

Diss Factsheets

Ecotoxicological information

Toxicity to microorganisms

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Administrative data

Endpoint:
activated sludge respiration inhibition testing
Type of information:
experimental study
Adequacy of study:
supporting study
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
guideline study with acceptable restrictions
Remarks:
OECD guideline study with some deviations. GLP compliant

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
1990
Report date:
1990

Materials and methods

Test guideline
Qualifier:
according to guideline
Guideline:
OECD Guideline 209 (Activated Sludge, Respiration Inhibition Test
Deviations:
yes
Remarks:
Deviating from the OECD Guideline 209 without 3,5-Dichlorophenol as reference substance and one used potassium dichromate. A concentration was used, which causes 50% inhibition of the Respiration.
GLP compliance:
yes

Test material

Constituent 1
Chemical structure
Reference substance name:
o-phenylenediamine
EC Number:
202-430-6
EC Name:
o-phenylenediamine
Cas Number:
95-54-5
Molecular formula:
C6H8N2
IUPAC Name:
benzene-1,2-diamine

Sampling and analysis

Analytical monitoring:
yes

Test organisms

Test organisms (species):
activated sludge of a predominantly domestic sewage

Study design

Test type:
not specified
Water media type:
not specified
Total exposure duration:
3 h

Results and discussion

Effect concentrationsopen allclose all
Duration:
3 h
Dose descriptor:
EC10
Effect conc.:
100 mg/L
Duration:
3 h
Dose descriptor:
other: EC20
Effect conc.:
100 mg/L
Duration:
3 h
Dose descriptor:
EC50
Effect conc.:
580 mg/L
Duration:
3 h
Dose descriptor:
other: EC80
Effect conc.:
> 1 000 mg/L

Any other information on results incl. tables

Remarks: No repetition of the analysis, since those exhibits test substance a weak increase in the process of the concentration ratio effect.

Criteria for the validity of the test:

1. Difference of the control respiration rates:< 15%

2. Inhibitor effect of the reference substance concentration: 40 - 60%

Reference substance: Potassium dichromate p.a. Merck

Test concentration: 60 [mg/L test medium]

Reference inhibition: 51 [%]

Validity criterion: Inhibition between 40 and 60%

Applicant's summary and conclusion

Conclusions:
EC10=100 mg/L, EC20=100 mg/L, EC50= 580 mg/L, EC80 = >1000 mg/L
Executive summary:

An activated sludge respiration inhibition test was conducted in accordance with OECD Guideline 209. The 3-hour EC50 was 580 mg/L.