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EC number: 203-113-5 | CAS number: 103-45-7
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data

Repeated dose toxicity: oral
Administrative data
- Endpoint:
- short-term repeated dose toxicity: oral
- Type of information:
- read-across from supporting substance (structural analogue or surrogate)
- Adequacy of study:
- weight of evidence
- Study period:
- August 1986
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- other: Published, high quality NTP range-finding study
- Justification for type of information:
- Based on structural, physicochemical and toxicity data, similarities between benzyl acetate is considered appropriate for use of the information by read across to phenyl ethyl acetate - see review attached in IUCLID section 13.2.
Cross-reference
- Reason / purpose for cross-reference:
- read-across source
Reference
- Endpoint:
- short-term repeated dose toxicity: oral
- Type of information:
- read-across from supporting substance (structural analogue or surrogate)
- Adequacy of study:
- weight of evidence
- Study period:
- August 1986
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- other: Published, high quality NTP range-finding study
- Justification for type of information:
- Based on structural, physicochemical and toxicity data, similarities between benzyl acetate is considered appropriate for use of the information by read across to phenyl ethyl acetate - see review attached in IUCLID section 13.2.
- Reason / purpose for cross-reference:
- read-across source
- Qualifier:
- no guideline followed
- Principles of method if other than guideline:
- Male and female B6C3F1 mice were obtained from Harlan Industries and observed for 12 days. Animals were approximately 6 weeks old when placed on study. Groups of five mice of each sex were administered 0, 125, 250, 500, 1,000, or 2,000 mg/ kg benzyl acetate in corn oil by gavage for 14 consecutive days. Animals were housed five per cage and received water and feed ad libitum. Mice were observed daily for mortality and were weighed weekly. On day 16, surviving animals were killed and necropsies were performed on all animals.
- GLP compliance:
- not specified
- Limit test:
- no
- Species:
- mouse
- Strain:
- B6C3F1
- Sex:
- male/female
- Details on test animals or test system and environmental conditions:
- TEST ANIMALS
- Source:Harlan Industries Indianapolis. I N
- Age at study initiation: 6 weeks
- Weight at study initiation: 19.2-28.5g
- Housing: Bedding: Beta-Chips heat treated hardwood chips
Cages: Polycarbonate
Cage filters: Spun-bonded polyester filters
- Diet (e.g. ad libitum): Wayne Lab-Blox Allied Mills. ad libitum
- Water (e.g. ad libitum): Tap water supplied through automatic watering system. ad libitum
- Acclimation period: 2 weeks
ENVIRONMENTAL CONDITIONS
- Temperature (°C): 21-23
- Humidity (%): 40-60
- Air changes (per hr): 15 - Route of administration:
- oral: gavage
- Vehicle:
- corn oil
- Details on oral exposure:
- PREPARATION OF DOSING SOLUTIONS: Doses were prepared on a weight-to-volume basis by pipetting the appropriate amount of benzyl acetate into a vessel and adding enough corn oil to give the desired concentration. Solutions were mixed until they were visually homogeneous. Mice received 10 ml/kg. Benzyl acetate/corn oil mixtures were analyzed at Midwest Research Institute and found to be stable at room temperature for at least 7 days. Once prepared, benzyl acetate/corn oil mixtures were stored at 5OC for no longer than 11 days.
VEHICLE
- Justification for use and choice of vehicle (if other than water): No data
- Concentration in vehicle: Male and female mice: 0, 125, 250, 500, 1,000 or 2,000 mg/kg,body weight in corn oil
- Amount of vehicle (if gavage): 10 ml/kg for mouse - Analytical verification of doses or concentrations:
- yes
- Details on analytical verification of doses or concentrations:
- Samples of benzyl acetate in corn oil were selected at random and analyzed periodically at Southern Research Institute. Results of these analyses and of referee analyses at Midwest Research Institute indicated that benzyl acetate/ corn oil mixtures were properly formulated.
- Duration of treatment / exposure:
- 14 consecutive days
- Frequency of treatment:
- Daily
- Remarks:
- Doses / Concentrations:
125mg/kg bw/day
Basis:
actual ingested - Remarks:
- Doses / Concentrations:
250mg/kg bw/day
Basis:
actual ingested - Remarks:
- Doses / Concentrations:
500mg/kg bw/day
Basis:
actual ingested - Remarks:
- Doses / Concentrations:
1000mg/kg bw/day
Basis:
actual ingested - Remarks:
- Doses / Concentrations:
2000mg/kg bw/day
Basis:
actual ingested - No. of animals per sex per dose:
- 5 animals
- Control animals:
- yes, concurrent vehicle
- Details on study design:
- - Dose selection rationale: based on an acute study
- Positive control:
- No
- Observations and examinations performed and frequency:
- CAGE SIDE OBSERVATIONS: Yes
- Time schedule: Observed daily for mortality and clinical signs.
DETAILED CLINICAL OBSERVATIONS: Yes
- Time schedule: Obserted daily for mortality and clinical signs.
BODY WEIGHT: Yes
- Time schedule for examinations: at the start and end of dosing
FOOD EFFICIENCY:
- Body weight gain in kg/food consumption in kg per unit time X 100 calculated as time-weighted averages from the consumption and body weight gain data: No
OPHTHALMOSCOPIC EXAMINATION: No
HAEMATOLOGY: No
CLINICAL CHEMISTRY: No
URINALYSIS: No
NEUROBEHAVIOURAL EXAMINATION: No
OTHER: Necropsies performed on all animals - Sacrifice and pathology:
- GROSS PATHOLOGY: Yes
HISTOPATHOLOGY: No - Other examinations:
- No further data
- Statistics:
- No data
- Clinical signs:
- effects observed, treatment-related
- Mortality:
- mortality observed, treatment-related
- Body weight and weight changes:
- no effects observed
- Food consumption and compound intake (if feeding study):
- not examined
- Food efficiency:
- not examined
- Water consumption and compound intake (if drinking water study):
- not examined
- Ophthalmological findings:
- not examined
- Haematological findings:
- not examined
- Clinical biochemistry findings:
- not examined
- Urinalysis findings:
- not examined
- Behaviour (functional findings):
- not examined
- Organ weight findings including organ / body weight ratios:
- not examined
- Gross pathological findings:
- effects observed, treatment-related
- Histopathological findings: non-neoplastic:
- not examined
- Histopathological findings: neoplastic:
- not examined
- Details on results:
- CLINICAL SIGNS AND MORTALITY All male mice that received 2,000 mg/ kg were dead by the afternoon of day 3. No other compound-related deaths occurred. Compound-related clinical signs were observed in high-dose males (ruffled fur, ataxia) and in high-dose females (labored breathing, hyperactivity).
BODY WEIGHT AND WEIGHT GAIN Weight changes were not dose related
GROSS PATHOLOGY Mucosa in the cardiac region of the stomach was roughened in 2/ 5 males and 5/ 5 females that received 2,000 mg/kg and in l / 5 females that received 1,000 mg/ kg. - Dose descriptor:
- NOAEL
- Effect level:
- ca. 1 000 mg/kg bw/day (nominal)
- Based on:
- test mat.
- Sex:
- male/female
- Basis for effect level:
- other: overall effects clinical signs; mortality in high dose group
- Critical effects observed:
- not specified
- Conclusions:
- The NOAEL was found to be 1000 mg/kg body weight after dosing mice with benzyl acetate in corn oil for 14 days.
- Executive summary:
Male and female B6C3F1 mice were obtained from Harlan Industries and observed for 12 days. Animals were approximately 6 weeks old when placed on study. Groups of five mice of each sex were administered 0, 125, 250, 500, 1,000, or 2,000 mg/ kg benzyl acetate in corn oil by gavage for 14 consecutive days. Animals were housed five per cage and received water and feed ad libitum. Mice were observed daily for mortality and were weighed weekly. On day 16, surviving animals were killed and necropsies were performed on all animals. The NOAEL was found to be 1000 mg/kg body weight after dosing mice with benzyl acetate in corn oil for 14 days.
Male/Female |
Dose |
Survival |
Final weight relative to control % |
Male |
0 |
4/5* |
- |
125 |
5/5 |
103.5 |
|
250 |
4/5* |
101.2 |
|
500 |
5/5 |
98.8 |
|
1000 |
4/5* |
120.8 |
|
2000 |
0/5 |
- |
|
Female |
0 |
5/5 |
- |
125 |
5/5 |
90.0 |
|
250 |
5/5 |
96.4 |
|
500 |
5/5 |
91.8 |
|
1000 |
0/5 |
90.9 |
|
2000 |
5/5* |
98.6 |
* deaths occured by gavage error
Data source
Reference
- Reference Type:
- publication
- Title:
- Toxicology and carcinogenesis of benzyl acetate (CAS NO. 140-11-4) in F344/N rats and B6C3F1 mice (gavage studies)
- Author:
- Abdo, K. et al
- Year:
- 1 986
- Bibliographic source:
- National Toxicology Program, Publication No. 86-2506, USA, 1986
Materials and methods
Test guideline
- Qualifier:
- no guideline followed
- Principles of method if other than guideline:
- Male and female B6C3F1 mice were obtained from Harlan Industries and observed for 12 days. Animals were approximately 6 weeks old when placed on study. Groups of five mice of each sex were administered 0, 125, 250, 500, 1,000, or 2,000 mg/ kg benzyl acetate in corn oil by gavage for 14 consecutive days. Animals were housed five per cage and received water and feed ad libitum. Mice were observed daily for mortality and were weighed weekly. On day 16, surviving animals were killed and necropsies were performed on all animals.
- GLP compliance:
- not specified
- Limit test:
- no
Test material
- Reference substance name:
- Benzyl acetate
- EC Number:
- 205-399-7
- EC Name:
- Benzyl acetate
- Cas Number:
- 140-11-4
- Molecular formula:
- C9H10O2
- IUPAC Name:
- benzyl acetate
Constituent 1
Test animals
- Species:
- mouse
- Strain:
- B6C3F1
- Sex:
- male/female
- Details on test animals or test system and environmental conditions:
- TEST ANIMALS
- Source:Harlan Industries Indianapolis. I N
- Age at study initiation: 6 weeks
- Weight at study initiation: 19.2-28.5g
- Housing: Bedding: Beta-Chips heat treated hardwood chips
Cages: Polycarbonate
Cage filters: Spun-bonded polyester filters
- Diet (e.g. ad libitum): Wayne Lab-Blox Allied Mills. ad libitum
- Water (e.g. ad libitum): Tap water supplied through automatic watering system. ad libitum
- Acclimation period: 2 weeks
ENVIRONMENTAL CONDITIONS
- Temperature (°C): 21-23
- Humidity (%): 40-60
- Air changes (per hr): 15
Administration / exposure
- Route of administration:
- oral: gavage
- Vehicle:
- corn oil
- Details on oral exposure:
- PREPARATION OF DOSING SOLUTIONS: Doses were prepared on a weight-to-volume basis by pipetting the appropriate amount of benzyl acetate into a vessel and adding enough corn oil to give the desired concentration. Solutions were mixed until they were visually homogeneous. Mice received 10 ml/kg. Benzyl acetate/corn oil mixtures were analyzed at Midwest Research Institute and found to be stable at room temperature for at least 7 days. Once prepared, benzyl acetate/corn oil mixtures were stored at 5OC for no longer than 11 days.
VEHICLE
- Justification for use and choice of vehicle (if other than water): No data
- Concentration in vehicle: Male and female mice: 0, 125, 250, 500, 1,000 or 2,000 mg/kg,body weight in corn oil
- Amount of vehicle (if gavage): 10 ml/kg for mouse - Analytical verification of doses or concentrations:
- yes
- Details on analytical verification of doses or concentrations:
- Samples of benzyl acetate in corn oil were selected at random and analyzed periodically at Southern Research Institute. Results of these analyses and of referee analyses at Midwest Research Institute indicated that benzyl acetate/ corn oil mixtures were properly formulated.
- Duration of treatment / exposure:
- 14 consecutive days
- Frequency of treatment:
- Daily
Doses / concentrationsopen allclose all
- Remarks:
- Doses / Concentrations:
125mg/kg bw/day
Basis:
actual ingested
- Remarks:
- Doses / Concentrations:
250mg/kg bw/day
Basis:
actual ingested
- Remarks:
- Doses / Concentrations:
500mg/kg bw/day
Basis:
actual ingested
- Remarks:
- Doses / Concentrations:
1000mg/kg bw/day
Basis:
actual ingested
- Remarks:
- Doses / Concentrations:
2000mg/kg bw/day
Basis:
actual ingested
- No. of animals per sex per dose:
- 5 animals
- Control animals:
- yes, concurrent vehicle
- Details on study design:
- - Dose selection rationale: based on an acute study
- Positive control:
- No
Examinations
- Observations and examinations performed and frequency:
- CAGE SIDE OBSERVATIONS: Yes
- Time schedule: Observed daily for mortality and clinical signs.
DETAILED CLINICAL OBSERVATIONS: Yes
- Time schedule: Obserted daily for mortality and clinical signs.
BODY WEIGHT: Yes
- Time schedule for examinations: at the start and end of dosing
FOOD EFFICIENCY:
- Body weight gain in kg/food consumption in kg per unit time X 100 calculated as time-weighted averages from the consumption and body weight gain data: No
OPHTHALMOSCOPIC EXAMINATION: No
HAEMATOLOGY: No
CLINICAL CHEMISTRY: No
URINALYSIS: No
NEUROBEHAVIOURAL EXAMINATION: No
OTHER: Necropsies performed on all animals - Sacrifice and pathology:
- GROSS PATHOLOGY: Yes
HISTOPATHOLOGY: No - Other examinations:
- No further data
- Statistics:
- No data
Results and discussion
Results of examinations
- Clinical signs:
- effects observed, treatment-related
- Mortality:
- mortality observed, treatment-related
- Body weight and weight changes:
- no effects observed
- Food consumption and compound intake (if feeding study):
- not examined
- Food efficiency:
- not examined
- Water consumption and compound intake (if drinking water study):
- not examined
- Ophthalmological findings:
- not examined
- Haematological findings:
- not examined
- Clinical biochemistry findings:
- not examined
- Urinalysis findings:
- not examined
- Behaviour (functional findings):
- not examined
- Organ weight findings including organ / body weight ratios:
- not examined
- Gross pathological findings:
- effects observed, treatment-related
- Histopathological findings: non-neoplastic:
- not examined
- Histopathological findings: neoplastic:
- not examined
- Details on results:
- CLINICAL SIGNS AND MORTALITY All male mice that received 2,000 mg/ kg were dead by the afternoon of day 3. No other compound-related deaths occurred. Compound-related clinical signs were observed in high-dose males (ruffled fur, ataxia) and in high-dose females (labored breathing, hyperactivity).
BODY WEIGHT AND WEIGHT GAIN Weight changes were not dose related
GROSS PATHOLOGY Mucosa in the cardiac region of the stomach was roughened in 2/ 5 males and 5/ 5 females that received 2,000 mg/kg and in l / 5 females that received 1,000 mg/ kg.
Effect levels
- Dose descriptor:
- NOAEL
- Effect level:
- ca. 1 000 mg/kg bw/day (nominal)
- Based on:
- test mat.
- Sex:
- male/female
- Basis for effect level:
- other: overall effects clinical signs; mortality in high dose group
Target system / organ toxicity
- Critical effects observed:
- not specified
Any other information on results incl. tables
Male/Female |
Dose |
Survival |
Final weight relative to control % |
Male |
0 |
4/5* |
- |
125 |
5/5 |
103.5 |
|
250 |
4/5* |
101.2 |
|
500 |
5/5 |
98.8 |
|
1000 |
4/5* |
120.8 |
|
2000 |
0/5 |
- |
|
Female |
0 |
5/5 |
- |
125 |
5/5 |
90.0 |
|
250 |
5/5 |
96.4 |
|
500 |
5/5 |
91.8 |
|
1000 |
0/5 |
90.9 |
|
2000 |
5/5* |
98.6 |
* deaths occured by gavage error
Applicant's summary and conclusion
- Conclusions:
- The NOAEL was found to be 1000 mg/kg body weight after dosing mice with benzyl acetate in corn oil for 14 days.
- Executive summary:
Male and female B6C3F1 mice were obtained from Harlan Industries and observed for 12 days. Animals were approximately 6 weeks old when placed on study. Groups of five mice of each sex were administered 0, 125, 250, 500, 1,000, or 2,000 mg/ kg benzyl acetate in corn oil by gavage for 14 consecutive days. Animals were housed five per cage and received water and feed ad libitum. Mice were observed daily for mortality and were weighed weekly. On day 16, surviving animals were killed and necropsies were performed on all animals. The NOAEL was found to be 1000 mg/kg body weight after dosing mice with benzyl acetate in corn oil for 14 days.
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.

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