C14-16 (even numbered) and C16 (branched) saturated and unsaturated aliphatic hydrocarbons

Sediment toxicity

Administrative data

Endpoint:

sediment toxicity: short-term

Type of information:

experimental study

Adequacy of study:

key study

Study period:

11/03/2005 - 21/03/2005

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

other: GLP not known, guideline study, acceptable with restrictions

Justification for type of information:

A discussion and report on the read across strategy is given as an attachment in Section 13.

Cross-reference

Reason / purpose for cross-reference:

read-across: supporting information

Reference

Endpoint:

sediment toxicity: short-term

Type of information:

read-across from supporting substance (structural analogue or surrogate)

Adequacy of study:

key study

Study period:

11/03/2005 - 21/03/2005

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

other: GLP not known, guideline study, acceptable with restrictions

Justification for type of information:

A discussion and report on the read across strategy is given as an attachment in Section 13.

Reason / purpose for cross-reference:

read-across source

Qualifier:

according to guideline

Guideline:

ASTM E1367 (10-day Static Sediment Toxicity Test (Media: Sediment-marine))

Version / remarks:

92

Deviations:

not specified

GLP compliance:

not specified

Specific details on test material used for the study:

Details on properties of test surrogate or analogue material (migrated information):
not applicable

Analytical monitoring:

no

Details on sampling:

not applicable

Vehicle:

no

Details on sediment and application:

not reported

Test organisms (species):

Leptocheirus plumulosus

Details on test organisms:

Source: cultured in natural sediment
Holding Conditions Prior to test: cultured and maintained in natural sediment acclimated to 20 ± 1 degrees C prior to test intitiation. Cultures fed during this time but not when test was initiated.

Study type:

laboratory study

Test type:

static

Water media type:

saltwater

Type of sediment:

artificial sediment

Limit test:

no

Duration:

10 d

Exposure phase:

total exposure duration

Post exposure observation period:

none

Hardness:

data not reported

Test temperature:

20 ± 1°C

pH:

data not reported

Dissolved oxygen:

data not reported

Salinity:

data not reported

Ammonia:

data not reported

Nominal and measured concentrations:

Replicates exposed to artificial sediment spiked with test substance at 5 different concentrations (150, 300, 600, 1200 and 2400 mg/kg dw) and negative controls. All treatments prepared and dispensed 18 to 24 hrs prior to intitiating test. Treatments kept in dedicated environmental chamber.

Details on test conditions:

aeration: yes
no of organisms per vessel: 20
no of replicates per concentration: 5 replicates
photoperiod: 14:10

Reference substance (positive control):

no

Duration:

10 d

Dose descriptor:

LC50

Effect conc.:

182 mg/kg sediment dw

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality

Details on results:

concurrent 10 day control survival was 96%

Reported statistics and error estimates:

Probit analysis

Validity criteria fulfilled:

not specified

Conclusions:

The 10 day LC50 was 182 mg/kg (dry weight sediment).

Executive summary:

This study followed a standard guideline. No detail on the composition of the sediment is reported, and analytical monitoring was not included. This result gives an indication of the levels of the test substance that may cause sediment toxicity.

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

2005

Report date:

2005

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

ASTM E1367 (10-day Static Sediment Toxicity Test (Media: Sediment-marine))

Version / remarks:

92

Deviations:

not specified

GLP compliance:

not specified

Test material

Test material information

Constituent 1

Reference substance name:

Reaction mass of Alkenes, C16 alpha-isomerized and Alkenes, C18

IUPAC Name:

Reaction mass of Alkenes, C16 alpha-isomerized and Alkenes, C18

Constituent 2

Reference substance name:

SBF CP Chem C16/18 1460-66

IUPAC Name:

SBF CP Chem C16/18 1460-66

Details on test material:

sample 25889-83 D

Specific details on test material used for the study:

Details on properties of test surrogate or analogue material (migrated information):
not applicable

Sampling and analysis

Analytical monitoring:

no

Details on sampling:

not applicable

Test substrate

Vehicle:

no

Details on sediment and application:

not reported

Test organisms

Test organisms (species):

Leptocheirus plumulosus

Details on test organisms:

Source: cultured in natural sediment
Holding Conditions Prior to test: cultured and maintained in natural sediment acclimated to 20 ± 1 degrees C prior to test intitiation. Cultures fed during this time but not when test was initiated.

Study design

Study type:

laboratory study

Test type:

static

Water media type:

saltwater

Type of sediment:

artificial sediment

Limit test:

no

Exposure duration

Duration:

10 d

Exposure phase:

total exposure duration

Post exposure observation period:

none

Test conditions

Hardness:

data not reported

Test temperature:

20 ± 1°C

pH:

data not reported

Dissolved oxygen:

data not reported

Salinity:

data not reported

Ammonia:

data not reported

Nominal and measured concentrations:

Replicates exposed to artificial sediment spiked with test substance at 5 different concentrations (150, 300, 600, 1200 and 2400 mg/kg dw) and negative controls. All treatments prepared and dispensed 18 to 24 hrs prior to intitiating test. Treatments kept in dedicated environmental chamber.

Details on test conditions:

aeration: yes
no of organisms per vessel: 20
no of replicates per concentration: 5 replicates
photoperiod: 14:10

Reference substance (positive control):

no

Results and discussion

Effect concentrations

Duration:

10 d

Dose descriptor:

LC50

Effect conc.:

182 mg/kg sediment dw

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality

Details on results:

concurrent 10 day control survival was 96%

Reported statistics and error estimates:

Probit analysis

Applicant's summary and conclusion

Validity criteria fulfilled:

not specified

Conclusions:

The 10 day LC50 was 182 mg/kg (dry weight sediment).

Executive summary:

This study followed a standard guideline. No detail on the composition of the sediment is reported, and analytical monitoring was not included. This result gives an indication of the levels of the test substance that may cause sediment toxicity.