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EC number: 700-762-0 | CAS number: -
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data

Genetic toxicity: in vitro
Administrative data
- Endpoint:
- in vitro gene mutation study in bacteria
- Remarks:
- Type of genotoxicity: gene mutation
- Type of information:
- migrated information: read-across from supporting substance (structural analogue or surrogate)
- Adequacy of study:
- key study
- Study period:
- 1995-09-22 to 1995-10-17
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- other: This study is classified as reliable without restriction because it was conducted in accordance with OECD 471 guideline for reversion assays and was GLP compliant.
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 1 996
- Report date:
- 1996
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 471 (Bacterial Reverse Mutation Assay)
- Deviations:
- yes
- Remarks:
- The identity, strength, purity, and stability of the test chemical were not provided in the study report.
- GLP compliance:
- yes
- Type of assay:
- bacterial reverse mutation assay
Test material
- Reference substance name:
- C14-16 (even numbered) and C16 (branched) saturated and unsaturated aliphatic hydrocarbons
- EC Number:
- 700-762-0
- Molecular formula:
- All molecules present in the mixture have the general molecular formula CnH2n for olefins or CnH2n+2 for the paraffin’s with n being an even number
- IUPAC Name:
- C14-16 (even numbered) and C16 (branched) saturated and unsaturated aliphatic hydrocarbons
- Test material form:
- liquid: viscous
Constituent 1
Method
- Target gene:
- Histidine
Species / strain
- Species / strain / cell type:
- S. typhimurium TA 1535, TA 1537, TA 98 and TA 100
- Metabolic activation:
- with and without
- Metabolic activation system:
- S-9
- Test concentrations with justification for top dose:
- 50, 158, 500, 1580, and 5000 micrograms per plate
- Vehicle / solvent:
- - Vehicle(s)/solvent(s) used: ethanol
- Justification for choice of solvent/vehicle: None provided
Controls
- Untreated negative controls:
- no
- Negative solvent / vehicle controls:
- yes
- True negative controls:
- no
- Positive controls:
- yes
- Positive control substance:
- benzo(a)pyrene
- Remarks:
- 2-nitrofluorene was also used as a positive control
- Details on test system and experimental conditions:
- METHOD OF APPLICATION: In agar (plate incorporation)
DURATION
- Preincubation period: None
- Exposure duration: 2 days
NUMBER OF REPLICATIONS: Three
DETERMINATION OF CYTOTOXICITY
- Method: other: background lawn - Evaluation criteria:
- Not reported.
- Statistics:
- None reported.
Results and discussion
Test results
- Species / strain:
- S. typhimurium TA 1535, TA 1537, TA 98 and TA 100
- Metabolic activation:
- with and without
- Genotoxicity:
- negative
- Cytotoxicity / choice of top concentrations:
- not specified
- Vehicle controls validity:
- valid
- Untreated negative controls validity:
- not examined
- Positive controls validity:
- valid
- Additional information on results:
- RANGE-FINDING/SCREENING STUDIES: An appropriate preliminary toxicity assay was performed to select an adequate dose range for testing in the mutagenicity assay.
- Remarks on result:
- other: all strains/cell types tested
- Remarks:
- Migrated from field 'Test system'.
Applicant's summary and conclusion
- Conclusions:
- Interpretation of results (migrated information):
negative
Based on these results, the study authors concluded that SHOP internal olefins C134 was not cytogenic to the four Salmonella typhimurium strains treated with concentrations up to 5000 µg/plate either in the presence or absence of S9. - Executive summary:
Justification for Read Across
Several criteria justify the use of the read across approach to fill data gaps for Category C substances using Category D substance analogues. Category C is comprised of isomerised olefins. Category D is comprised of isomerised olefins with a range of carbon numbers. Studies indicate that changing the carbon number or the location of the double bond, or adding branching does not measurably alter the mammalian health endpoints. There appears to be no critical difference across Category D isomerised olefins with a range of carbon numbers with regard to health endpoints. Toxicity concerns are low for acute exposure to Category D substances. Genotoxicity studies indicate that these materials are not mutagenic. There was no adverse systemic toxicity observed in a 90-day repeated oral dose study, in which rats were exposed to C20-24 branched and linear alkenes. As a result, all of the above tested mammalian toxicity endpoints indicate a low hazard potential for human health. There do not appear to be any toxicological differences between Category D isomerised olefins with a range of carbon numbers and Category C is comprised of isomerised olefins. Therefore, read across between these two categories can be justified.
In a reverse gene mutation assay in bacteria, four strains of Salmonella typhimurium (TA 98, 100, 1535, and 1537) were exposed to SHOP internal olefins C134 dissolved in ethanol at concentrations of 50, 158, 500, 1580, and 5000 µg/plate in the presence and absence of mammalian S-9 metabolic activation using a plate incorporation assay and incubated for 2 days. An appropriate preliminary toxicity assay was performed to select an adequate dose range for testing in the mutagenicity assay. The test conditions appear to comply with OECD 471 guideline requirements. Positive and negative controls indicated that the test system was working appropriately. None of the test chemical treated bacterial strains showed increases in reversions. Based on these results, the study authors concluded that SHOP internal olefins C134 was not cytogenic to the four Salmonella typhimurium strains treated with concentrations up to 5000 µg/plate either in the presence or absence of S9.
This study received a Klimisch score of 1 and is classified as reliable without restrictions because it was conducted in accordance with OECD 471 guideline for reversion assays and was GLP compliant. This study will influence the DNEL.
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