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Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.

The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.

Diss Factsheets

Administrative data

Endpoint:
dermal absorption in vitro / ex vivo
Type of information:
experimental study
Adequacy of study:
supporting study
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
other: Comparable to Guideline study with acceptable restrictions (one dose measured [screening of numerous substances]).

Data source

Reference
Reference Type:
publication
Title:
Percutaneous absorption of explosives and related compounds: an empirical model of bioavailability of organic nitro compounds from soil
Author:
Reifenrath WG, Kammen HO, Palmer WG, Major MM, Leach GJ
Year:
2002
Bibliographic source:
Toxicol Appl Pharmacol 182: 160–168

Materials and methods

Test guideline
Qualifier:
equivalent or similar to guideline
Guideline:
OECD Guideline 428 (Skin Absorption: In Vitro Method)
Deviations:
no
GLP compliance:
not specified

Test material

Constituent 1
Chemical structure
Reference substance name:
Thiodiglycol
EC Number:
203-874-3
EC Name:
Thiodiglycol
Cas Number:
111-48-8
Molecular formula:
C4H10O2S
IUPAC Name:
2-[(2-hydroxyethyl)sulfanyl]ethan-1-ol
Details on test material:
[14C(U)]-thiodiglycol, specific activity of 12.33 mCi/mmol in ethanol solution (9.94 mg/ml) (prepared by Wizard Laboratories); radiochemical purity was 96.6–99.2% by thin-layer chromatography.
Radiolabelling:
yes
Remarks:
14C

Test animals

Details on test animals or test system and environmental conditions:
in vitro

Administration / exposure

Duration of exposure:
24 h
Doses:
10.2+-0.5 µg/cm²
Details on study design:
Acetone solutions of radiolabeled compound prepared so that a 5-µl volume would contain approximately 8 µg of compound.
Details on in vitro test system (if applicable):
SKIN PREPARATION
- Source of skin: female Yorkshire pigs, weighing 30 to 50 kg
- Type of skin: upper back
- Preparative technique: whole skin was harvested
- Thickness of skin (in mm): 0.5-0.9
- Membrane integrity check: no data

- Diffusion cell: low volume flow cell
- Solubility od test substance in receptor fluid: yes

Results and discussion

Percutaneous absorption
Dose:
10.2 µg/cm²
Parameter:
percentage
Absorption:
ca. 20 %
Remarks on result:
other: 24 h

Any other information on results incl. tables

Percent applied radioactive dose (mean of 5 replicates)

Evaporation: 30 +-11%

Skin decontamination: 17 +-6%

Epidermis: 24 +-11%

Dermis: 12 +-9%

Receptor fluid: 7 +-3%

Total recovery: 90 +-6%

Percutaneous absorption of 14C-radiolabeled thiodiglycol in acetone solution: 20 +-9% of applied dose (n=5 replicates; 10 µg/cm² compound).

Applicant's summary and conclusion

Conclusions:
In vitro experiments revealed a percutaneous absorption of 20%.
Executive summary:

Comparable to Guideline study with acceptable restrictions (one dose measured [screening of numerous substances]).

Percutaneous absorption was studied in diffusion cells using harvested pig skin of the back. After application of 10.2 µg/cm²

14C-labelled thiodiglycol in acetone solution radioactivity was measured in dermis, epidermis and receptor fluid after 24 h, but also in skin decontamination and evaporation. The total recovery was 90% (n=5 replicates). The following amounts of radioactivity were measured: evaporation 30 +-11%, skin decontamination 17 +-6%, epidermis: 24 +-11%, dermis: 12 +-9%, receptor fluid: 7 +-3%.

Conclusion: In vitro experiments revealed a percutaneous absorption of 20%.