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Ecotoxicological information

Short-term toxicity to aquatic invertebrates

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Administrative data

Endpoint:
short-term toxicity to aquatic invertebrates
Type of information:
experimental study
Adequacy of study:
key study
Study period:
1994
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
other: Test procedure according to National Standards without detailed documentation.

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
1994
Report date:
1994

Materials and methods

Test guideline
Qualifier:
according to guideline
Guideline:
other: AFNOR T90301
Deviations:
no
GLP compliance:
not specified

Test material

Constituent 1
Reference substance name:
Valproic Acid
IUPAC Name:
Valproic Acid
Constituent 2
Chemical structure
Reference substance name:
2-propylvaleric acid
EC Number:
202-777-3
EC Name:
2-propylvaleric acid
Cas Number:
99-66-1
Molecular formula:
C8H16O2
IUPAC Name:
2-propylpentanoic acid
Details on test material:
- Name of test material (as cited in study report): Acide valproique pur (pure valproic acid)
- Lot/batch No.: 4MXS017

Sampling and analysis

Analytical monitoring:
not specified

Test solutions

Vehicle:
no

Test organisms

Test organisms (species):
Daphnia magna
Details on test organisms:
Name: Daphnia magna Straus, 1820 (Cladocera crustacea), less than 24h-old.
Source: Laboratoire INERIS

Study design

Test type:
static
Water media type:
freshwater
Limit test:
no
Total exposure duration:
48 h
Remarks on exposure duration:
in darkness

Test conditions

Reference substance (positive control):
yes
Remarks:
Potassium dichromate

Results and discussion

Effect concentrations
Duration:
48 h
Dose descriptor:
EC50
Effect conc.:
122.8 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mobility
Remarks on result:
other: 72.7-207.0
Results with reference substance (positive control):
EC(I)50-24h for Potassium Dichromate
from 1994-07-18 to 1994-07-22 = 1.06mg/L,
from 1994-07-25 to 1994-07-29 = 0.96mg/L,
from 1994-08-01 to 1994-08-05 = 1.34mg/L,
from 1994-08-08 to 1994-08-12 = 1.14mg/L

Any other information on results incl. tables

Valproïc acid:

Concentrations (mg/L)

Number of immobilized daphnia after 48 h

% immobilisation

(total: 20)

pH

O2 (mg/L)

A

B

C

D

Total

0

0

0

0

0

0

0

 

 

65

2

2

0

1

5

25

 

 

91

2

1

1

0

4

20

 

 

128

1

1

1

2

5

25

 

 

179

4

4

5

4

17

85

 

 

250

5

5

5

5

20

100

5.71

7.2

 

Applicant's summary and conclusion

Validity criteria fulfilled:
yes
Conclusions:
With the 48h-EC 50 for Daphnia magna > 100 mg/l the substance is not considered harmful to aquatic invertebrates