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Ecotoxicological information

Toxicity to aquatic algae and cyanobacteria

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Administrative data

Endpoint:
toxicity to aquatic algae and cyanobacteria
Type of information:
experimental study
Adequacy of study:
key study
Reliability:
1 (reliable without restriction)
Rationale for reliability incl. deficiencies:
guideline study

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
2007
Report date:
2007

Materials and methods

Test guideline
Qualifier:
according to guideline
Guideline:
OECD Guideline 201 (Alga, Growth Inhibition Test)
Deviations:
no
GLP compliance:
yes (incl. QA statement)

Test material

Constituent 1
Chemical structure
Reference substance name:
Sodium valproate
EC Number:
213-961-8
EC Name:
Sodium valproate
Cas Number:
1069-66-5
Molecular formula:
C8H16O2.Na
IUPAC Name:
sodium 2-propylpentanoate

Sampling and analysis

Analytical monitoring:
yes

Test solutions

Vehicle:
yes

Test organisms

Test organisms (species):
Pseudokirchneriella subcapitata (previous names: Raphidocelis subcapitata, Selenastrum capricornutum)
Details on test organisms:
Algae
Species: Pseudokirchneriella subcapitata.
Strain No.: CCAP 278/4.
Reason for this choice: species commonly used in Europe for aquatic toxicity testing and
recommended in OECD and EEC guidelines.
Cultured at: CIT.
Origin: Culture Collection of Algae and Protozoa (CCAP), SAMS Research

Study design

Test type:
static
Water media type:
freshwater
Limit test:
no
Total exposure duration:
72 h

Test conditions

Hardness:
34 ± 17 mg/L as CaCO3
Test temperature:
Between 23.4°C and 25°C
pH:
8.10 to 9.24
Nominal and measured concentrations:
0.01, 0.1, 1, 10, 100 mg/L (nominal concentration)

Results and discussion

Effect concentrationsopen allclose all
Key result
Duration:
72 h
Dose descriptor:
other: ErC50
Effect conc.:
> 100 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
growth rate
Duration:
72 h
Dose descriptor:
other: EbC50
Effect conc.:
> 100 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
other: growth
Key result
Duration:
72 h
Dose descriptor:
NOEC
Effect conc.:
ca. 100 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
growth rate

Any other information on results incl. tables

The minimum and maximum parameters measured during the test were:

• pH: 8.10 and 9.24 (8.11 to 9.15 for the control),

• temperature: 23.4°C and 25.0°C,

• lighting: 7.020 lux and 7580 lux.

All validity criteria of the study were respected for each test.

No significant (p = 0.05) inhibition of the algal growth was observed at 100 mg/L and the measured concentrations of the test item were within ± 20% of this nominal value throughout the test.

Applicant's summary and conclusion

Validity criteria fulfilled:
yes
Conclusions:
In a 72-hour algal growth inhibition test with Pseudokirchneriella subcapitata, the EC50 of Sodium Valproate was > 100 mg/L based on growth rate (ErC50) and growth (EbC50).
The NOEC was ≥ 100 mg/L.