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Ecotoxicological information

Toxicity to soil microorganisms

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Administrative data

Endpoint:
toxicity to soil microorganisms
Type of information:
experimental study
Adequacy of study:
other information
Reliability:
4 (not assignable)
Rationale for reliability incl. deficiencies:
documentation insufficient for assessment

Data source

Reference
Reference Type:
publication
Title:
The Effect of Both Powdered and Liquid Rubber Additives on the Growth of Soil Microorganisms
Author:
Williams GR
Year:
1984
Bibliographic source:
International Biodetoriation Vol. 20 No. 3

Materials and methods

Principles of method if other than guideline:
Step No. 1: Soil burial experiment with three different burial techniques to examine whether or not the test substance support microbial growth.
Step No. 2: The substance which did not support microbial growth was incorporated into nutrient agar, at varying concentrations, approximating the levels of incorporation in rubber formulations, and inoculated with soil extract (3.0 E+07 organisms per ml) at 25°C for 14 days.
GLP compliance:
not specified

Test material

Reference
Name:
Unnamed
Type:
Constituent
Details on test material:
no data on purity

Sampling and analysis

Analytical monitoring:
not specified

Test organisms

Test organisms (inoculum):
soil

Results and discussion

Effect concentrationsopen allclose all
Duration:
4 d
Dose descriptor:
other: LD50
Effect conc.:
0.25 other: %
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
other: growth inhibition
Duration:
14 d
Dose descriptor:
other: LD50
Effect conc.:
0.25 other: %
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
other: growth inhibition

Any other information on results incl. tables

In step No.1 , CBS did not support the soil microbial growth after three months soil burial.

In step No. 2, the substance was classified as moderately toxic.

Applicant's summary and conclusion

Executive summary:

After three monthes soil burial experiment, CBS did not support the growth of soil microbes (Williams, 1984). In further toxicity experiment soil extract was incubated at 25°C for 14 days in nutrient agar with CBS incorporated. A LD50 of 0.25% was reported and thus CBS was classified as moderately toxic to soil microbes (Williams, 1984).