Calcium dodecylbenzenesulphonate

Biodegradation in water and sediment: simulation tests

Link to relevant study record(s)

Reference

Reference 1

Endpoint:

biodegradation in water: sewage treatment simulation testing

Type of information:

read-across from supporting substance (structural analogue or surrogate)

Adequacy of study:

weight of evidence

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

study well documented, meets generally accepted scientific principles, acceptable for assessment

Justification for type of information:

C12 LAS; Linear Alkylbenzene Sulfonate (LAS)) is a very close analogue of Calcium dodecylbenzenesulfonate (CAS No 26264-06-2, EC Number; 247-557-8) ) and read-across is valid.

Reason / purpose for cross-reference:

read-across source

Qualifier:

no guideline followed

Principles of method if other than guideline:

Radiolabeled LAS was dosed at an environmentally relevant concentration into biotic and abiotic activated sludge. Activated sludge mixed liquor was used from two STP (Polk Run and Sycamore) near Cincinnati. Standard methods were used and the test flasks (reactors) were maintained at 20+/-2oC.

GLP compliance:

not specified

Specific details on test material used for the study:

14C-[uniform ring] C12 LAS; radiochemical purity exceeded 98% based upon TLC.14C-[uniform ring] C12 LAS was obtained from New England Nuclear(Boston, MA) an had a specific activity of 68 mCi/mg.

Radiolabelling:

yes

Oxygen conditions:

aerobic

Inoculum or test system:

activated sludge, non-adapted

Details on source and properties of sediment:

Test medium :Activated sludge mixed liquor was collected from Pol Run Sewage Treatment (STP) and Sycamore Sewage Treatmnet (STP) which are located near on 1995. Both treatment plants receive mainly domestic wastewater and are located near Cincinnati, OH.

Duration of test (contact time):

52 h

Initial conc.:

1 mg/L

Based on:

test mat.

Details on study design:

14C-ring C12 LAS homologs is dosed at an environmentally relevant concentration into biotic and abiotic activated sludge.Activated sludge mixed liquor was collected from Pol Run Sewage Treatment(STP) and Sycamore Sewage Treatmnet(STP)Periodically, samples are removed, acidified, and analyzed by LSC(Liquid Scintillation Counting) to determine 14 CO2 evolution. And then the 14C-ring C12 LAS homologs are flahfrozen, lyophilized, and extracted to recover the parent and metabolites, which are analyzed by Rad-TLC.

Compartment:

other: water / sediment, material (mass) balance

Remarks on result:

other: PolK Run Sewage Treatment(STP) and Sycamore Sewage Treatmnet(STP)

% Degr.:

> 96

Parameter:

test mat. analysis

Sampling time:

6 h

Compartment:

entire system

DT50:

1.3 h

Type:

(pseudo-)first order (= half-life)

Remarks on result:

other: Polk Run STP

Compartment:

entire system

DT50:

1.38 h

Type:

(pseudo-)first order (= half-life)

Remarks on result:

other: Sycamore STP

Transformation products:

yes

Details on results:

-Within 8h, 41-44% was evolved as 14CO2 , 1-2% remained as parent, 24-33% was incorporated into biomass, and 10-15% was present as intermediates, primarily sulfophenylcarboxylates.- Primary and complete biodegradation were described by a first-order model,with rate constants of 0.96-1.10/h (t 1/2 = 0.63–0.72 h) for primaryloss and 0.50-0.53/h (t 1/2 = 1.30–1.38 h) for complete degradation.

Validity criteria fulfilled:

yes

Conclusions:

Primary and complete biodegradation were described by a first-order model, with rate constants of 0.96-1.10/h (t½ = 0.63–0.72 h) for primary loss and 0.50-0.53/h (t½ = 1.30–1.38 h) for complete degradation.

Reference 2

Endpoint:

biodegradation in water: sewage treatment simulation testing

Type of information:

read-across from supporting substance (structural analogue or surrogate)

Adequacy of study:

weight of evidence

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

study well documented, meets generally accepted scientific principles, acceptable for assessment

Justification for type of information:

Linear Alkylbenzene Sulfonate (LAS) is a very close analogue of Calcium dodecylbenzenesulfonate (CAS No 26264-06-2, EC Number; 247-557-8) and read-across is valid.

Reason / purpose for cross-reference:

read-across source

Qualifier:

equivalent or similar to guideline

Guideline:

OECD Guideline 303 A (Simulation Test - Aerobic Sewage Treatment. A: Activated Sludge Units)

GLP compliance:

not specified

Specific details on test material used for the study:

Dodecylbenzenesulfonate 14C(DBS-14C)

Radiolabelling:

yes

Oxygen conditions:

anaerobic

Inoculum or test system:

activated sludge (adaptation not specified)

Duration of test (contact time):

120 h

Initial conc.:

76 other: ppm

Based on:

test mat.

Details on study design:

This study showed the biodegradation of LAS (DBS-14C) and the decomposition products of DBS-14C. The composition products of DBS-14C were identified by GLC and GC-MS spectrometry.(GLC column ; 0.3 cm×1.7 m, stationary phase; PEG-20M, temp; 200 deg C). And The biodegradation values of DBS-14C was obtained by methlene blue method.

% Degr.:

> 90

Parameter:

test mat. analysis

Sampling time:

90 h

Transformation products:

yes

No.:

#1

No.:

#2

No.:

#3

No.:

#4

Details on transformation products:

1-Tetralone, 1-indanone, 4-methyl-1-tetralone, naphthalene were the decomposition products.

Details on results:

DBS decreased from 76.0 ppm to less than 0.6 ppmat 90h(Readily degradation).The biodegradation of DBS-14C has three periods of rapid adsorption period, acclimation period, and degradation process. 1-Tetralone, 1-indanone, 4-methyl-1-tetralone, naphthalene were the decomposition products

Validity criteria fulfilled:

yes

Conclusions:

DBS decreased from 76.0 ppm to less than 0.6 ppmat 90h(Readily degradation).The biodegradation of DBS-14C has three periods of rapid adsorption period, acclimation period, and degradation process. 1-Tetralone, 1-indanone, 4-methyl-1-tetralone, naphthalene were the decomposition products

Reference 3

Endpoint:

biodegradation in water: sediment simulation testing

Data waiving:

study scientifically not necessary / other information available

Justification for data waiving:

the study does not need to be conducted because the substance is readily biodegradable

Description of key information

Biodegradation in water and sediment: simulation tests
In the study of Federle TW and Itrich NR 1997, Linear alkylbenzene sulfonate (14C-ring C12LAS) was tested as model compounds in two sludges.
-	Within 8 h, 41−44% was evolved as 14CO2, 
-	1−2% remained as parent, 
-	24−33% was incorporated into biomass, 
-	and 10−15% was present as intermediates, primarily sulfophenylcarboxylates.
 Primary and complete biodegradation were best described by a first-order model. First-order rate constants for LAS were 0.96−1.10 h-1 for primary loss and 0.50−0.53 h-1 for complete degradation. This approach provides an accurate and comprehensive kinetic picture of biodegradation under realistic conditions as well as information on the mechanism of biodegradation.
Biodegradation of Linear alkylbenzene sulfonate (14C-ring C12LAS), at 1 mg/l was measured >96% after 6 hours and test temperature was maintained at 20 deg C.
In the study of Kubodera T, Muto T and Yamamoto T 1978 Dodecylbenzenesulfonate 14C (DBS-14C) was tested as model compound.
Biodegradation of Dodecylbenzenesulfonate 14C(DBS-14C) at 76 ppm was measured >90% after 90 hours and test temperature was maintained at 24 deg C.
DBS decreased from 76.0 ppm to less than 0.6 ppm at 90h (Readily degradation).The biodegradation of DBS-14C has three periods of rapid adsorption period, acclimation period, and degradation process. 1-Tetralone, 1-indanone, 4-methyl-1-tetralone, naphthalene were the decomposition products

Additional information

Biodegradation in water and sediment: simulation tests

 

In the study of Federle TW and Itrich NR 1997, Linear alkylbenzene sulfonate (14C-ring C12LAS) was tested as model compounds in two sludges.

-        Within 8 h, 41−44% was evolved as 14CO2,

-        1−2% remained as parent,

-        24−33% was incorporated into biomass,

-        and 10−15% was present as intermediates, primarily sulfophenylcarboxylates.

 Primary and complete biodegradation were best described by a first-order model. First-order rate constants for LAS were 0.96−1.10 h-1 for primary loss and 0.50−0.53 h-1 for complete degradation. This approach provides an accurate and comprehensive kinetic picture of biodegradation under realistic conditions as well as information on the mechanism of biodegradation.

 

Biodegradation ofLinear alkylbenzene sulfonate (14C-ring C12LAS), at 1mg/lwas measured>96% after6hoursand test temperature was maintained at 20 deg C.

 

In the study of Kubodera T, Muto T and Yamamoto T 1978 Dodecylbenzenesulfonate 14C (DBS-14C) was tested as model compound.

Biodegradation of Dodecylbenzenesulfonate 14C(DBS-14C)at76ppmwas measured>90% after90hoursand test temperature was maintained at 24deg C.

DBS decreased from 76.0 ppm to less than 0.6 ppm at 90h (Readily degradation).The biodegradation of DBS-14C has three periods of rapid adsorption period, acclimation period, and degradation process. 1-Tetralone, 1-indanone, 4-methyl-1-tetralone, naphthalene were the decomposition products