Calcium dodecylbenzenesulphonate

Toxicity to soil macroorganisms except arthropods

Link to relevant study record(s)

Reference

Endpoint:

toxicity to soil macroorganisms except arthropods: long-term

Type of information:

read-across from supporting substance (structural analogue or surrogate)

Adequacy of study:

weight of evidence

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

study well documented, meets generally accepted scientific principles, acceptable for assessment

Justification for type of information:

CAS: 42615-29-2, Benzenesulfonic acid, linear alkyl is a very close analogue of Calcium dodecylbenzenesulfonate (CAS No 26264-06-2, EC Number; 247-557-8) ) and read-across is valid.

Reason / purpose for cross-reference:

read-across source

Qualifier:

according to guideline

Guideline:

OECD Guideline 207 (Earthworm, Acute Toxicity Tests)

Principles of method if other than guideline:

OECD Guideline 207, 1984. Ten adult worms (mean wt. 0.66 g/animal) were placed into each of four glass jars per concentration with soil comprised of 70% 5010 grade silica sand, 20% kaolinite clay, and 10% finely ground sphagnum peat. Nominal test concentrations in the soil were 1000, 500, 250, 125, 63 and 0 mg/kg dry weight. Temperature was maintained at 20+/-2oCwith 24-hour continuous lighting at 600 lux. Earthworms were assessed for mortality, general health, body weight, and behavior after 7 and 14 days.

GLP compliance:

not specified

Analytical monitoring:

yes

Vehicle:

yes

Test organisms (species):

Eisenia fetida

Animal group:

annelids

Details on test organisms:

Species :Earthworm (Eisenia foetida), mean weight 660 mg
No. of worms: 10 worms/jar, 4 jars/treatment.

Study type:

laboratory study

Substrate type:

artificial soil

Limit test:

no

Total exposure duration:

14 d

Post exposure observation period:

Earthworms were assessed for mortality, general health, body weight and behavior after 7 and 14 days.

Test temperature:

20±2℃

Moisture:

35±1%.

Details on test conditions:

- Soil : 70% silica sand, 20% kaolinite clay and 10% sphagnum peat- Soil Temperature: 20 +/- 2 deg C- Soil moisture : 35%- Lamp: cool white fluorescence lamps, 700-750 lux (24 hours)

Nominal and measured concentrations:

norminally 1000, 500, 250, 125, 63, and 1 microgram/gram dry weight

Duration:

14 d

Dose descriptor:

LC50

Effect conc.:

> 1 000 mg/kg soil dw

Nominal / measured:

meas. (initial)

Conc. based on:

test mat.

Basis for effect:

mortality

Remarks:

Mortality, Weight

Remarks on result:

other: Eisenia foetida / LAS : 14days-LC50 > 1mg/g. (nominal).

Duration:

14 d

Dose descriptor:

NOEC

Effect conc.:

250 mg/kg soil dw

Nominal / measured:

meas. (initial)

Conc. based on:

test mat.

Basis for effect:

mortality

Remarks:

Mortality, Weight

Details on results:

All group had 100% wurvival except the 1000 mg/kg which had 95% survival. A 33% and 23 % reduction in body weight was pbserved at 100 and 500 mg/kg. Based on statistical analysis of the weight data, the no effect concentration was nominal 250 mg/kg dose, which was confirmed by HPLC to be 235 mg/kg.

Measured concentration was 94% of nominal.

Parameter	Time (d)	Nominal concentration [mg/kg soil]
		0	63	125	250	500	1000
Mortality [%]	14	0	0	0	0	0	5

 

Validity criteria fulfilled:

yes

Conclusions:

All group had 100% wurvival except the 1000 mg/kg which had 95% survival. A 33% and 23 % reduction in body weight was pbserved at 100 and 500 mg/kg. Based on statistical analysis of the weight data, the no effect concentration was nominal 250 mg/kg dose, which was confirmed by HPLC to be 235 mg/kg.
The 14d-LC50for Eisenia foetida was more than 1000 mg/kg.

Executive summary:

An earthworm acute toxicity test (OECD TG 207) of commercial LAS (average carbon chain length of 11.36) was performed with Eisenia foetida on artificial soil. The 14d-LC₅₀for Eisenia foetida was more than 1000 mg/kg.

Description of key information

An earthworm acute toxicity test (OECD TG 207) of commercial LAS (average carbon chain length of 11.36) was performed with Eisenia foetida on artificial soil. The 14d-LC50 for Eisenia foetida was more than 1000 mg/kg. The No effect concentration was nominal NOEC=250 mg/kg soil.
In another study used Lumbricus terrestris for 14days and 14d-LC50 value was more than 1333 mg/kg. The no effect concentration was nominal NOEC=667 mg/kg soil.

Key value for chemical safety assessment

Short-term EC50 or LC50 for soil macroorganisms:

1 000 mg/kg soil dw

Long-term EC10, LC10 or NOEC for soil macroorganisms:

250 mg/kg soil dw

Additional information

An earthworm acute toxicity test (OECD TG 207) of commercial LAS (average carbon chain length of 11.36) was performed with Eisenia foetida on artificial soil. The 14d-LC50 for Eisenia foetida was more than 1000 mg/kg.

The No effect concentration was nominal NOEC=250 mg/kg soil.

In another study used Lumbricus terrestris for 14 days and 14d-LC50 value was more than 1333 mg/kg. The no effect concentration was nominal NOEC=667 mg/kg soil.