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Short-term toxicity to aquatic invertebrates

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Reference
Endpoint:
short-term toxicity to aquatic invertebrates
Type of information:
experimental study
Adequacy of study:
key study
Study period:
1988-06-15 to 1988-06-17
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
other: Test procedure in accordance with national standard methods with acceptable restrictions: Exposure period only 24 hours, incomplete documentation
Qualifier:
according to guideline
Guideline:
other: DIN 38412 part 11
Deviations:
not specified
GLP compliance:
no
Analytical monitoring:
no
Vehicle:
no
Details on test solutions:
STOCK AND TEST SOLUTION AND THEIR PREPARATION
- Concentration: 10 g/l, no solvent or vehicle
Test organisms (species):
Daphnia magna
Details on test organisms:
TEST ORGANISMS
- Strain: Daphnia magna, Hüls
- Source/supplier: Hüls AG (inhouse)
- Breeding method: in 1 l jars with dechlorinated drinking water, water  renewal each 2-3 days, isolation of juveniles for further breeding ca every
4 weeks
- Age: < 24 hours
- Feeding: Chlorella vulgaris, daily as much as consumed
- Pretreatment: Removal of adults 24 h prior to testing
- Feeding during test: no
Test type:
static
Water media type:
freshwater
Limit test:
no
Total exposure duration:
24 h
Test temperature:
20 +/- 1 degree C
Nominal and measured concentrations:
12; 18; 25; 35; 50; 70; 100 mg/l (nominal)
Details on test conditions:
TEST SYSTEM
- Exposure vessel type: 25 ml graduated cylinder
- Number of replicates, individuals per replicate: 4 replicates with 5 individuals each
- Control group: 2 reference substance controls, one blank
- Photoperiod: dark

DILUTION WATER
- Source: Synthetic: CaCl2 x 2 H2O: 294 mg/l; MgSO4 x 7 H2O: 123 mg/l; NaHCO3: 63 mg/l; KCl: 5.5 mg/l
- Ca/Mg ratio: 4:1
- Na/K ratio: 10:1
- Aeration: no

TEST PARAMETER: immobilization
Reference substance (positive control):
yes
Remarks:
potassium dichromate, CAS RN 7778-50-9
Duration:
24 h
Dose descriptor:
EC0
Effect conc.:
25 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mobility
Duration:
24 h
Dose descriptor:
EC50
Effect conc.:
31.5 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mobility
Remarks on result:
other: 28.9-34.2
Duration:
24 h
Dose descriptor:
EC100
Effect conc.:
70 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mobility
Details on results:
- EC50 values were calculated according to Cavalli-Sforza (1972)
- the mortality data are presented below (remarks on results)
Results with reference substance (positive control):
- Concentrations: 0.9 mg/l / 1.9 mg/l
- Results: 30 % immobile / 85 % immobile (24 hours)

RESULTS: 
- Effect data (Immobilisation): No. of immobile daphnia
  Control:   0 (24 h)
   12 mg/l:  0 (24 h) 
   18 mg/l:  0 (24 h)
   25 mg/l:  2 (24 h)
   35 mg/l: 16 (24 h)
   50 mg/l: 19 (24 h) 
   70 mg/l: 20 (24 h) 
  100 mg/l: 20 (24 h) 
  EC50, 95 % confidence interval: 28.9 - 34.2 mg/l (24 hours)

Validity criteria fulfilled:
not specified
Conclusions:
The EC50 (24 hours) was determined as 31.5 mg/L indicating that the test substance may be harmful to aquatic invertebrates.
Executive summary:

The acute toxicity of the test item on Daphnia magna was investigated by Hüls AG (1996) in a 24 -h test according to national guidance documents (DIN 38412, part II). The following endpoint was derived:

EC50 (24 hours): 31.5 mg/L; CL: 28.9 mg/L - 34.2 mg/L.

The study was assessed as "reliable with restrictions".

Description of key information

The acute toxicity of the test item on Daphnia magna was investigated by Hüls AG (1996) in a 24 -h test according to national guidance documents (DIN 38412, part II). The EC50 (24 hours) was determined as 31.5 mg/L (CL: 38.9 - 34.2 mg/L) indicating that the test substance may be harmful to 
aquatic invertebrates.

Key value for chemical safety assessment

Fresh water invertebrates

Fresh water invertebrates
Dose descriptor:
EC50
Effect concentration:
31.5 mg/L

Additional information