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Long-term toxicity to aquatic invertebrates

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Description of key information

The Daphnia magna Reproduction Test (semi-static, 21 d) was conducted according to OECD 211 (2012). Test species was Daphnia magna STRAUS (Clone 5). Ten daphnids, individually held, were used per concentration level, and control. The study was carried out under semi-static conditions with concentrations ranging from 1.02 to 40.0 mg/l. The measured test item concentrations were all within ± 20 % of the nominal concentrations throughout the exposure period indicating that the test item concentrations were successfully maintained for the duration of the test.
Aim of the Daphnia Reproduction Test over 21 days was to assess effects on the reproduction capacity and other test item-related effects or parameters such as intrinsic rate of natural increase, number of broods with living juveniles, first appearance of living juveniles, occurrence of aborted eggs and stillborn juveniles, adult mortality and body length of the parental daphnids.The adult mortality was the most sensitive endpoint in this study. Based on the significant adult mortality at the concentration levels of 2.56 to 40.0 mg/L, the No Observed Effect Concentration (NOEC) after 21 days was assessed as 1.02 mg/L and theLowest Observed Effect Concentration (LOEC)was assessed as 2.56 mg/L. Based on the nominal test item concentrations, the EC10was determined as 1.02 mg/L. The EC50was calculated to be 5.26 mg/L (95% confidence limits: 2.23 – 11.8 mg/L). Due to the lack of significant effects, no EC-values for the reproduction could be calculated.

Key value for chemical safety assessment

Fresh water invertebrates

Fresh water invertebrates
Effect concentration:
1.02 mg/L

Additional information