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Ecotoxicological information

Toxicity to soil macroorganisms except arthropods

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Description of key information

Effects or the test item on mortality, growth and the reproductive potential of the earthworm species Eisenia fetida were determined according to OECD Guideline 222 (2004) from May 15th to July 11th, 2015 at Dr. U.Noack-Laboratorien in Sarstedt, Germany. The study was conducted over 8 weeks with test item concentrations of 62.5, 125, 250, 500 and 1000 mg test item/kg dw, which were mixed into artificial soil. A control containing untreated artificial soil was also set up. Sexually mature earthworms were inserted into 8 control replicates and into 4 replicates per test item concentration (10 earthworms per replicate).
After 28 days of exposure in soil, no earthworm mortalities, pathological symptoms or changes in the behaviour of adult earthworms were observed in any treatment. No statistically significant differences in growth (earthworm body weight gain) were observed at any test item concentration.
After a further four weeks, the reproduction rate (mean number of juveniles produced per replicate) was 118 in the control and ranged from 118 to 142 in the test item treatment replicates. Compared to the control, no statistically significant reduction of reproduction was observed in any test item concentration. Consequently, the NOEC of the test item concerning growth and reproduction was determined to be ≥ 1000 mg/kg dw.

Key value for chemical safety assessment

Long-term EC10, LC10 or NOEC for soil macroorganisms:
1 000 mg/kg soil dw

Additional information