2-methylpyridine

Short-term toxicity to aquatic invertebrates

Administrative data

Endpoint:

short-term toxicity to aquatic invertebrates

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

other: Published in peer reviewed literature, minor restrictions in design and/or reporting but otherwise adequate for assessment

Data source

Reference

Reference Type:

publication

Title:

Heterocyclic compounds: toxic effects using algae, daphnids, and the salmonella/microsome test taking methodical quantitative aspects into account

Author:

Eisentraeger et al.

Year:

2008

Bibliographic source:

Environmental Toxicology and Chemistry, Vol. 27, No. 7, pp. 1590–1596, 2008

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

ISO 6341 (Water quality - Determination of the Inhibition of the Mobility of Daphnia magna Straus (Cladocera, Crustacea))

GLP compliance:

not specified

Test material

Test material information

Constituent 1

Reference substance name:

2-methylpyridine

EC Number:

203-643-7

EC Name:

2-methylpyridine

Cas Number:

109-06-8

Molecular formula:

C6H7N

IUPAC Name:

2-methylpyridine

Details on test material:

- Name of test material (as cited in study report): 2-methylpyridine

Sampling and analysis

Analytical monitoring:

yes

Details on sampling:

- Sampling method: to determine the real concentrations in the test treatments, according to the Organization for Economic Cooperation and Development (OECD) 201 samples of the lowest, middle, and the highest concentrations were taken at the beginning and the end of the bioassays.

Test solutions

Vehicle:

yes

Details on test solutions:

The compound was mixed with 500 mL of 100% dimethylsulfoxide (DMSO, Merck) and eluted for 24 h at room temperature. The solution was stored in Duran glass bottles (Schott Rohrglas, Mitterteich, Germany) at 4°C for up to one month in the dark.

Test organisms

Test organisms (species):

Daphnia magna

Details on test organisms:

TEST ORGANISM
- Common name: Water flea
- Strain: Straus

Study design

Test type:

not specified

Water media type:

freshwater

Limit test:

no

Total exposure duration:

24 h

Test conditions

Nominal and measured concentrations:

- Nominal concentrations: 80, 200, and 500 mg/L
- Measured concentrations (t=0): 77.78, 224.38, and 553.53 mg/L
- Measured concentrations (t=24): 76.12, 194.02, and 68.48 mg/L

Details on test conditions:

- No. of vessels per concentration (replicates): 2

Reference substance (positive control):

no

Results and discussion

Effect concentrations

Effect concentrations 1

Duration:

24 h

Dose descriptor:

EC50

Effect conc.:

221.4 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mobility

Remarks on result:

other: 95% C.L: 119.8-411.3 mg/L

Effect concentrations 2

Duration:

24 h

Dose descriptor:

EC50

Effect conc.:

107.1 mg/L

Nominal / measured:

meas. (arithm. mean)

Conc. based on:

test mat.

Basis for effect:

mobility

Remarks on result:

other: 95% C.L: 58.0-199.3 mg/L

Details on results:

Only minor effects were observed when the daphnids were exposed.

Reported statistics and error estimates:

With the measured values, the median concentration during the assay was determined. A calibration curve was constructed with these values so that concentration values for the probit analysis were interpolated using the maximum likelihood solution. With these interpolated values, median EC50 values have been calculated. Confidence intervals were calculated using the equation from Finney and Weber.