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Toxicological information

Genetic toxicity: in vitro

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Administrative data

Endpoint:
genetic toxicity in vitro
Type of information:
experimental study
Adequacy of study:
supporting study
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
other: Non guideline study. GLP-status unknown. Published in peer reviewed literature. Restrictions in design and reporting but adequate for assessment.

Data source

Referenceopen allclose all

Reference Type:
publication
Title:
Genetic and anti-tubulin effects induced by pyridine derivatives
Author:
Zimmermann F.K., Henning J.H., Scheel I., Oehler, M.
Year:
1986
Bibliographic source:
Mutation Research, 163, 23-31
Reference Type:
review article or handbook
Title:
SCIENTIFIC OPINION Scientific opinion on Flavouring Group Evaluation 24, Revision 2 (FGE.24Rev2): Pyridine, pyrrole, indole and quinoline derivatives from chemical group 28
Author:
European Food Safety Authority (EFSA)
Year:
2013
Bibliographic source:
EFSA Journal 2013;11(11):3453

Materials and methods

Principles of method if other than guideline:
The test substance was evaluated for its potential to induce mitotic aneuploidy, mitotic recombination and anti-tubulin effects in yeast strain S. cerevisiae D61.M.
GLP compliance:
not specified

Test material

Constituent 1
Chemical structure
Reference substance name:
2-methylpyridine
EC Number:
203-643-7
EC Name:
2-methylpyridine
Cas Number:
109-06-8
Molecular formula:
C6H7N
IUPAC Name:
2-methylpyridine

Method

Species / strain
Species / strain / cell type:
Saccharomyces cerevisiae
Metabolic activation:
without
Test concentrations with justification for top dose:
0.50, 0.55, 0.60, 0.65, 0.70, 074 % (Highest dose tested 6988 μg/mL)
Controls
Untreated negative controls:
no
Negative solvent / vehicle controls:
yes
True negative controls:
no
Positive controls:
no
Details on test system and experimental conditions:
- The test substance was tested in a liquid yeast-peptone medium. The cell titers at the time of addition were between 1 and 2E7 cells per mL.
- Incubation in the presence of the test substance was carried out at 28°C for 4 h. The cultures were then placed into an ice bath and kept overnight for about 16-17 h. A final growth period of another 4 h at 28°C followed before the cells were plated.

Results and discussion

Test results
Species / strain:
Saccharomyces cerevisiae
Metabolic activation:
without
Genotoxicity:
positive
Cytotoxicity / choice of top concentrations:
not specified
Vehicle controls validity:
valid
Untreated negative controls validity:
not examined
Positive controls validity:
not examined
Additional information on results:
Induction of chromosome loss and other genetic changes: weakly active. This effect was only observed at very high doses. The effect is however considered thresholded (EFSA Journal 2013;11(11):3453).
Induction of anti-tubulin effects: not induced
Remarks on result:
other: all strains/cell types tested
Remarks:
Migrated from field 'Test system'.

Applicant's summary and conclusion

Conclusions:
Interpretation of results (migrated information):
positive At high concentrations