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EC number: 203-643-7 | CAS number: 109-06-8
Genetic toxicity: in vitro
Administrative data
- Endpoint:
- in vitro gene mutation study in bacteria
- Remarks:
- Type of genotoxicity: gene mutation
- Type of information:
- experimental study
- Adequacy of study:
- supporting study
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- other: Non guideline study. GLP-status unknown. Published in peer reviewed literature. Limitations in design and reporting, but adequate for assessment.
Data source
Referenceopen allclose all
- Reference Type:
- publication
- Title:
- Contribution of coffee aroma constituents to the mutagenicity of coffee
- Author:
- Aeschbacher H.U., Wolleb U., Loliger J., Spadone J.C., Liardon R.
- Year:
- 1 989
- Bibliographic source:
- Food Chem. Toxicol. 27(4), 227-232
- Reference Type:
- review article or handbook
- Title:
- SCIENTIFIC OPINION Scientific opinion on Flavouring Group Evaluation 24, Revision 2 (FGE.24Rev2): Pyridine, pyrrole, indole and quinoline derivatives from chemical group 28
- Author:
- European Food Safety Authority (EFSA)
- Year:
- 2 013
- Bibliographic source:
- EFSA Journal 2013;11(11):3453
Materials and methods
- Principles of method if other than guideline:
- Over 40 coffee aroma constituents, among which the test substance, were systematically subjected to the three most sensitive Ames tester strains, TA98, TA100 and TA102 with and without metabolic activation.
- GLP compliance:
- not specified
- Type of assay:
- bacterial reverse mutation assay
Test material
- Reference substance name:
- 2-methylpyridine
- EC Number:
- 203-643-7
- EC Name:
- 2-methylpyridine
- Cas Number:
- 109-06-8
- Molecular formula:
- C6H7N
- IUPAC Name:
- 2-methylpyridine
- Details on test material:
- - Name of test material (as cited in study report): 2-Methylpyridine
Constituent 1
Method
- Target gene:
- His-locus
Species / strain
- Species / strain / cell type:
- S. typhimurium, other: TA98, TA100, TA102
- Metabolic activation:
- with and without
- Metabolic activation system:
- Aroclor 1254-induced rat livers
- Test concentrations with justification for top dose:
- 10 nmol - 1 mmol/plate (6 dose levels)
- Vehicle / solvent:
- - Vehicle(s)/solvent(s) used: Water
Controlsopen allclose all
- Untreated negative controls:
- no
- Negative solvent / vehicle controls:
- yes
- True negative controls:
- no
- Positive controls:
- yes
- Positive control substance:
- 2-nitrofluorene
- sodium azide
- mitomycin C
- Remarks:
- Without metabolic activation
- Untreated negative controls:
- no
- Negative solvent / vehicle controls:
- yes
- True negative controls:
- no
- Positive controls:
- yes
- Positive control substance:
- other: 2-aminofluorene
- Remarks:
- With metabolic activation
- Details on test system and experimental conditions:
- METHOD OF APPLICATION: preincubation
DURATION
- Exposure duration: 72 hour
NUMBER OF REPLICATIONS: 4 plates per test concentration
DETERMINATION OF CYTOTOXICITY
- Method: pre-assay using TA100 was carried out to determine the highest non-bactericidal dose level.
OTHER:
- Mutation factors (MF) (induced/spontaneous revertants) were calculated at the dose levels that give the greatest effect.
Results and discussion
Test results
- Species / strain:
- S. typhimurium, other: TA98, TA100, TA102
- Metabolic activation:
- with and without
- Genotoxicity:
- negative
- Cytotoxicity / choice of top concentrations:
- not specified
- Vehicle controls validity:
- not specified
- Untreated negative controls validity:
- not specified
- Positive controls validity:
- not specified
- Remarks on result:
- other: all strains/cell types tested
- Remarks:
- Migrated from field 'Test system'.
Applicant's summary and conclusion
- Conclusions:
- Interpretation of results (migrated information):
negative
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