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Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.

The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.

Diss Factsheets

Toxicological information

Genetic toxicity: in vitro

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Administrative data

Endpoint:
in vitro gene mutation study in bacteria
Remarks:
Type of genotoxicity: gene mutation
Type of information:
experimental study
Adequacy of study:
supporting study
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
other: Non guideline study. GLP-status unknown. Published in peer reviewed literature. Limitations in design and reporting, but adequate for assessment.

Data source

Referenceopen allclose all

Reference Type:
publication
Title:
Contribution of coffee aroma constituents to the mutagenicity of coffee
Author:
Aeschbacher H.U., Wolleb U., Loliger J., Spadone J.C., Liardon R.
Year:
1989
Bibliographic source:
Food Chem. Toxicol. 27(4), 227-232
Reference Type:
review article or handbook
Title:
SCIENTIFIC OPINION Scientific opinion on Flavouring Group Evaluation 24, Revision 2 (FGE.24Rev2): Pyridine, pyrrole, indole and quinoline derivatives from chemical group 28
Author:
European Food Safety Authority (EFSA)
Year:
2013
Bibliographic source:
EFSA Journal 2013;11(11):3453

Materials and methods

Principles of method if other than guideline:
Over 40 coffee aroma constituents, among which the test substance, were systematically subjected to the three most sensitive Ames tester strains, TA98, TA100 and TA102 with and without metabolic activation.
GLP compliance:
not specified
Type of assay:
bacterial reverse mutation assay

Test material

Constituent 1
Chemical structure
Reference substance name:
2-methylpyridine
EC Number:
203-643-7
EC Name:
2-methylpyridine
Cas Number:
109-06-8
Molecular formula:
C6H7N
IUPAC Name:
2-methylpyridine
Details on test material:
- Name of test material (as cited in study report): 2-Methylpyridine

Method

Target gene:
His-locus
Species / strain
Species / strain / cell type:
S. typhimurium, other: TA98, TA100, TA102
Metabolic activation:
with and without
Metabolic activation system:
Aroclor 1254-induced rat livers
Test concentrations with justification for top dose:
10 nmol - 1 mmol/plate (6 dose levels)
Vehicle / solvent:
- Vehicle(s)/solvent(s) used: Water
Controlsopen allclose all
Untreated negative controls:
no
Negative solvent / vehicle controls:
yes
True negative controls:
no
Positive controls:
yes
Positive control substance:
2-nitrofluorene
sodium azide
mitomycin C
Remarks:
Without metabolic activation
Untreated negative controls:
no
Negative solvent / vehicle controls:
yes
True negative controls:
no
Positive controls:
yes
Positive control substance:
other: 2-aminofluorene
Remarks:
With metabolic activation
Details on test system and experimental conditions:
METHOD OF APPLICATION: preincubation

DURATION
- Exposure duration: 72 hour

NUMBER OF REPLICATIONS: 4 plates per test concentration

DETERMINATION OF CYTOTOXICITY
- Method: pre-assay using TA100 was carried out to determine the highest non-bactericidal dose level.

OTHER:
- Mutation factors (MF) (induced/spontaneous revertants) were calculated at the dose levels that give the greatest effect.

Results and discussion

Test results
Species / strain:
S. typhimurium, other: TA98, TA100, TA102
Metabolic activation:
with and without
Genotoxicity:
negative
Cytotoxicity / choice of top concentrations:
not specified
Vehicle controls validity:
not specified
Untreated negative controls validity:
not specified
Positive controls validity:
not specified
Remarks on result:
other: all strains/cell types tested
Remarks:
Migrated from field 'Test system'.

Applicant's summary and conclusion

Conclusions:
Interpretation of results (migrated information):
negative