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Toxicological information

Acute Toxicity: oral

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Administrative data

Endpoint:
acute toxicity: oral
Type of information:
experimental study
Adequacy of study:
key study
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
other: Non GLP, non guideline study. Published in peer reviewed literature. Minor restrictions in design and reporting but adequate for assessment.

Data source

Reference
Reference Type:
publication
Title:
Comparative acute toxicity and primary irritancy of various classes of amines
Author:
Myers, R.C., and Ballantyne, B.
Year:
1997
Bibliographic source:
Toxic Substance Mechanisms 16, 151-193

Materials and methods

Principles of method if other than guideline:
Male Wistar albino rats were orally exposed to the test substance by gavage followed by a 14 day observation period. Besides mortality, animal weight was recorded. Necropsy was performed on all animals that died and on sacrificed survivors to examine for gross pathology.
GLP compliance:
not specified
Test type:
standard acute method
Limit test:
no

Test material

Constituent 1
Chemical structure
Reference substance name:
2-methylpyridine
EC Number:
203-643-7
EC Name:
2-methylpyridine
Cas Number:
109-06-8
Molecular formula:
C6H7N
IUPAC Name:
2-methylpyridine
Details on test material:
- Name of test material (as cited in study report): a-Picoline (2-methyl-pyridine)
- Physical state: Liquid

Test animals

Species:
rat
Strain:
Wistar
Sex:
male
Details on test animals or test system and environmental conditions:
TEST ANIMALS
- Age at study initiation: 3-4 wk
- Weight at study initiation: 90-120 g
- Fasting period before study: No fasting took place
- Diet: Commercial rodent feed, ad libitum
- Water: Municipal water, ad libitum

Administration / exposure

Route of administration:
oral: gavage
Vehicle:
unchanged (no vehicle)
No. of animals per sex per dose:
5
Control animals:
not specified
Details on study design:
- Duration of observation period following administration: 14 days
- Frequency of observations and weighing: On the day of dosing, and 14 d post dosing.
- Necropsy of survivors performed: yes
- Other examinations performed: Gross pathology
Statistics:
LD50 values and their 95% confidence limits were calculated by the moving average method of Thompson (1947) and the tables of Weil (1983).

Results and discussion

Effect levels
Sex:
male
Dose descriptor:
LD50
Effect level:
885 mg/kg bw
95% CL:
600 - 1 314
Remarks on result:
other: Value reported 0.93 (0.63-1.38) mL/kg
Mortality:
Animals died within 4 hours after dosing.
Clinical signs:
- Rapid progression of lethargy, laboured breathing, ataxia, and prostration, all occurring within a few minutes.
- Convulsions at day 1.
Body weight:
No effects observed
Gross pathology:
- Animals that died: lung haemorrhage, congested kidneys, and hyperaemic stomachs and/or intestines.
- Animals that survived: most survivors showed no gross pathology, a few had lung haemorrhages.

Applicant's summary and conclusion

Interpretation of results:
harmful
Remarks:
Migrated information