2-nitrophenol

Specific investigations: other studies

Administrative data

Endpoint:

methaemoglobinaemia

Type of information:

experimental study

Adequacy of study:

supporting study

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

other: Scientifically acceptable data

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1970

Report date:

1970

Materials and methods

Test guideline

Qualifier:

no guideline followed

Principles of method if other than guideline:

Acute toxicity study with cats. Single application: p.o.; Observation: 14 days, Additionally MetHb-Analysis was done.

GLP compliance:

no

Type of method:

in vivo

Endpoint addressed:

acute toxicity: oral

Test material

Test material information

Constituent 1

Reference substance name:

2-nitrophenol

EC Number:

201-857-5

EC Name:

2-nitrophenol

Cas Number:

88-75-5

Molecular formula:

C6H5NO3

IUPAC Name:

2-nitrophenol

Details on test material:

- Name of test material (as cited in study report): o-Nitrophenol
- Physical state: solid
no further data provided

Test animals

Species:

cat

Strain:

not specified

Sex:

male/female

Details on test animals or test system and environmental conditions:

TEST ANIMALS
- Source: Gewerbehyg.-Pharmakol. Institute of BASF
- Weight at study initiation: mean: males: 2.1kg; females: 2.0 kg

Administration / exposure

Route of administration:

oral: gavage

Vehicle:

other: 2 and 10% aqueous suspension with traganth

Details on exposure:

VEHICLE
- Concentration in vehicle: 2.5 and 0.5%

Analytical verification of doses or concentrations:

not specified

Duration of treatment / exposure:

single treatment

Frequency of treatment:

once

Post exposure period:

7 days

Doses / concentrations

Remarks:

Doses / Concentrations:
50, 100, 250 mg/kg bw
Basis:
actual ingested

No. of animals per sex per dose:

2 cats each dose level (high and low dose group one male and one female cat, mid dose group two male animlas)

Control animals:

no

Examinations

Examinations:

- Determination of haemoglobin in blood with spectrophotometric method of Evelyn and Malloy.
- Toxic symptoms
- Necropsy of dead cats

Results and discussion

Details on results:

Clinical symptoms: 250 mg/kg bw: cyanosis, lateral position, atony, convulsions, increased respiration, yellow staining of the iris and ear tips (from the substance), one cat died after 2 days;
100 mg/kg bw: cyanosis
50 mg/kg bw: one animal showed increased respiration
Met-Hb:
250 mg/kg bw: up to 57% (4 h after exposure)
100 mg/kg bw: up to 44% (2 h after exposure)
50 mg/kg bw: up to 6% (2 h after exposure)

Met-Hb amounts were measured every two hours. After 24 hours all Met-Hb levels normalised again.

Necropsy of dead animal: urinary bladder was tightly filled with yellow-green-brownish urine (colour of substance). Necropsy of the other animals was not done.

Applicant's summary and conclusion

Conclusions:

Animals treated with 250 and 100 mg/kg bw via gavage showed an induction of Methaemoglobin after 2 hours of treatment. This substance induces Met-Hb production.