2-nitrophenol

Dermal absorption

Administrative data

Endpoint:

dermal absorption

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

other: Scientifically acceptable publication.

Data source

Reference

Reference 1

Reference Type:

publication

Title:

Permeation of Water Contaminative Phenols Through Hairless Mouse Skin

Author:

Huq AS, Ho NFH, Husari N, Flynn GL, Jetzer WE, Condie Jr. L

Year:

1986

Bibliographic source:

Arch. Environ. Contam. Toxicol. 15, 557-566

Reference 2

Reference Type:

secondary source

Title:

No information

Author:

Rhone-Poulnec Chimie Courbevoie Cedex

Year:

1977

Bibliographic source:

IUCLID Chemical Data Sheet, edited 2000-02-19

Materials and methods

Test guideline

Qualifier:

no guideline available

Principles of method if other than guideline:

The study was conducted with hairless mouse skin as a model for the assessment of permeation kinetics.

GLP compliance:

not specified

Test material

Test material information

Constituent 1

Reference substance name:

2-nitrophenol

EC Number:

201-857-5

EC Name:

2-nitrophenol

Cas Number:

88-75-5

Molecular formula:

C6H5NO3

IUPAC Name:

2-nitrophenol

Details on test material:

- Name of test material (as cited in study report): 2-Nitrophenol
No further details are given on the test material.

Radiolabelling:

no

Test animals

Species:

mouse

Strain:

SKH/HR1

Sex:

not specified

Details on test animals or test system and environmental conditions:

TEST ANIMALS
- Age at study initiation: 60-100 days old

Administration / exposure

Type of coverage:

other: not applicable

Vehicle:

other: not applicable

Duration of exposure:

no data

Doses:

0.05 g/100 ml

No. of animals per group:

no data

Details on in vitro test system (if applicable):

After sacrificing a mouse by spinal cord dislocation, its abdominal skin was wiped clean with a tissue moistened with normal saline. Rectangular sections of whole skin several cm in each dimension excised with surgical scissors. Adhering fat and other visceral debris were removed carefully from the undersurface with tweezers. The excised skin was trimmed into oversized circles and mounted carefully between the half-cells of the two-compartment diffusion cell and fastened with a spring clamp. The two-half cells were filled with normal saline, the whole system was immersed in a waterbath (37 °C) and the stirring motors were connected. After the cell compartments were rinsed with normal saline, the donor compartment were charged with appropiate concentrations of the test substance.
At predetermined times, samples were withdrawn from the receiver compartment and analysed by direct UV spectrophotometry. Each sample was replaced with fresh saline with correction of the receiver concentration for the dilution of sampling. Samples were taken from the donor compartment at the beginning and end of each experiment to check for mass balance.

Results and discussion

Absorption in different matrices:

The test substance was found to have an apparent permeability coefficient of 101.0 (± 6.9) x 10³ cm/hr at a pH 3.46 in the donor-compartment and pH 6.2 in the receiver-compartment.