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Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.

The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.

Diss Factsheets

Toxicological information

Dermal absorption

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Administrative data

Endpoint:
dermal absorption
Type of information:
experimental study
Adequacy of study:
key study
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
other: Scientifically acceptable publication.

Data source

Referenceopen allclose all

Reference Type:
publication
Title:
Permeation of Water Contaminative Phenols Through Hairless Mouse Skin
Author:
Huq AS, Ho NFH, Husari N, Flynn GL, Jetzer WE, Condie Jr. L
Year:
1986
Bibliographic source:
Arch. Environ. Contam. Toxicol. 15, 557-566
Reference Type:
secondary source
Title:
No information
Author:
Rhone-Poulnec Chimie Courbevoie Cedex
Year:
1977
Bibliographic source:
IUCLID Chemical Data Sheet, edited 2000-02-19

Materials and methods

Test guideline
Qualifier:
no guideline available
Principles of method if other than guideline:
The study was conducted with hairless mouse skin as a model for the assessment of permeation kinetics.
GLP compliance:
not specified

Test material

Constituent 1
Chemical structure
Reference substance name:
2-nitrophenol
EC Number:
201-857-5
EC Name:
2-nitrophenol
Cas Number:
88-75-5
Molecular formula:
C6H5NO3
IUPAC Name:
2-nitrophenol
Details on test material:
- Name of test material (as cited in study report): 2-Nitrophenol
No further details are given on the test material.
Radiolabelling:
no

Test animals

Species:
mouse
Strain:
SKH/HR1
Sex:
not specified
Details on test animals or test system and environmental conditions:
TEST ANIMALS
- Age at study initiation: 60-100 days old

Administration / exposure

Type of coverage:
other: not applicable
Vehicle:
other: not applicable
Duration of exposure:
no data
Doses:
0.05 g/100 ml
No. of animals per group:
no data
Details on in vitro test system (if applicable):
After sacrificing a mouse by spinal cord dislocation, its abdominal skin was wiped clean with a tissue moistened with normal saline. Rectangular sections of whole skin several cm in each dimension excised with surgical scissors. Adhering fat and other visceral debris were removed carefully from the undersurface with tweezers. The excised skin was trimmed into oversized circles and mounted carefully between the half-cells of the two-compartment diffusion cell and fastened with a spring clamp. The two-half cells were filled with normal saline, the whole system was immersed in a waterbath (37 °C) and the stirring motors were connected. After the cell compartments were rinsed with normal saline, the donor compartment were charged with appropiate concentrations of the test substance.
At predetermined times, samples were withdrawn from the receiver compartment and analysed by direct UV spectrophotometry. Each sample was replaced with fresh saline with correction of the receiver concentration for the dilution of sampling. Samples were taken from the donor compartment at the beginning and end of each experiment to check for mass balance.

Results and discussion

Absorption in different matrices:
The test substance was found to have an apparent permeability coefficient of 101.0 (± 6.9) x 10³ cm/hr at a pH 3.46 in the donor-compartment and pH 6.2 in the receiver-compartment.

Applicant's summary and conclusion